A5103273535- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Rare Diseases
- Viral Infections and Vectors
- Zebrafish Biomedical Research Applications
- Cell death mechanisms and regulation
- RNA and protein synthesis mechanisms
- Air Quality and Health Impacts
- Chromosomal and Genetic Variations
- Cancer-related gene regulation
- Genomics, phytochemicals, and oxidative stress
- Peroxisome Proliferator-Activated Receptors
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Ocean Waves and Remote Sensing
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
Ningxia Medical University
2012-2025
Jilin Agricultural University
2020-2023
Liaocheng University
2020-2023
Army Medical University
2022-2023
Shandong Normal University
2023
Dalian Medical University
2022
Chinese Academy of Sciences
2022
Nanjing Medical University
2022
Yangzhou University
2014-2022
Jiangsu Cancer Hospital
2022
Abstract Background Study on long non-coding RNAs (lncRNAs) has been promoted by high-throughput RNA sequencing (RNA-Seq). However, it is still not trivial to identify lncRNAs from the RNA-Seq data and remains a challenge uncover their functions. Results We present computational pipeline for detecting novel data. First, genome-guided transcriptome reconstruction used generate initially assembled transcripts. The possible partial transcripts artefacts are filtered according quantified...
Functional long non-coding RNAs (lncRNAs) have been bringing novel insight into biological study, however it is still not trivial to accurately distinguish the lncRNA transcripts (LNCTs) from protein coding ones (PCTs). As various information and data about lncRNAs are preserved by previous studies, appealing develop methods identify more accurately. Our method lncRScan-SVM aims at classifying PCTs LNCTs using support vector machine (SVM). The gold-standard datasets for model training,...
We investigated the potential ability of quercetin to protect against lipopolysaccharide (LPS)-induced intestinal oxidative stress in broiler chickens and role Nrf2 (nuclear factor erythroid 2-related 2) signaling pathway. One-day-old (n = 240) were randomized into four groups: saline-challenged fed a basal diet (Con), LPS-challenged on (LPS), LPS-treated containing either 200 or 500 mg/kg (Que200+LPS Que500+LPS). Quercetin (200 mg/kg) significantly alleviated LPS-induced decreased duodenal,...
KLF1 (formerly known as EKLF) regulates the development of erythroid cells from bi-potent progenitor via transcriptional activation a diverse set genes. Mice lacking Klf1 die in utero prior to E15 severe anemia due inadequate expression genes controlling hemoglobin production, cell membrane and cytoskeletal integrity, cycle. We have recently described full repertoire binding sites vivo by performing ChIP-seq primary tissue (E14.5 fetal liver). Here we describe KLF1-dependent transcriptome...
Highlights•COMA, COMB, and COMC complexes are required for ETC CIII assembly•COMB comprises of microproteins BRAWNIN SMIM4 promotes nascent stability•COMB tunes levels according to nutrient nuclear subunit availability•Early assembly is a node cells sense respond mitochondrial stressSummaryElectron transport chain (ETC) biogenesis tightly coupled energy availability subunits. Complex III (CIII), controlling ubiquinol:ubiquinone ratio in ETC, an attractive modulating during metabolic stress....
Lipid droplets (LDs) are phylogenetically conserved cytoplasmic organelles that store neutral lipids within a phospholipid monolayer. LDs compartmentalize and may help to prevent cellular damage caused by their excess or bioactive forms. FIT2 is ubiquitously expressed transmembrane endoplasmic reticulum (ER) membrane protein has previously been implicated in LD formation mammalian cells tissue. Recent data indicate plays an essential role fat storage vivo constitutive adipose knock-out mouse...
Congenital heart disease (CHD) is the leading non-infectious cause of death in infants. Monozygotic (MZ) twins share nearly all their genetic variants before and after birth. Nevertheless, MZ are sometimes discordant for common complex diseases. The goal this study to identify genomic epigenomic differences between a pair form congenital disease, double outlet right ventricle (DORV). A monoamniotic monozygotic twin DORV were subjected genome-wide sequencing methylation analysis. We...
Cordycepin, an important active substance in Cordyceps militaris, possesses antiviral and other beneficial activities. In addition, it has been reported to effectively promote the comprehensive treatment of COVID-19 thus become a research hotspot. The addition naphthalene acetic acid (NAA) is known significantly improve yield cordycepin; however, its related molecular mechanism remains unclear. We conducted preliminary study on C. militaris with different concentrations NAA. found that NAA...
Cylindromatosis (CYLD), a deubiquitinase involved in inflammation and tumorigenesis via the modulation of cell signaling, has recently been identified as critical regulator microtubule dynamics. CYLD also shown to stimulate migration thereby contribute normal physiological processes. However, it remains elusive how regulation dynamic properties by is connected its role mediating migration. In this study, we performed yeast 2-hybrid screening with bait 7 CYLD-interacting proteins, including...
Abstract Background GenoLab M is a recently established next-generation sequencing platform from GeneMind Biosciences. Presently, Illumina sequencers are the globally leading in market. Here, we present first report to compare transcriptome and LncRNA data of sequencer NovaSeq 6000 various types analysis. Results We tested 16 libraries three species using library kits different companies. compared quality, genes expression, alternatively spliced (AS) events, single nucleotide polymorphism...
The effects of ambient fine particles on male reproductive health have raised widespread concern. particular underlying mechanisms the damage remain largely unclear and demand more research in new directions. Previous has revealed that DNA methylation plays an important role development is also vulnerable to environmental influences. However, there hasn't been enough investigation into involvement PM2.5-induced toxicity. Here, we establish a real-time PM2.5 exposure model could lead...
Targeted genomic sequencing (TS) greatly benefits precision oncology by rapidly detecting genetic variations with better accuracy and sensitivity owing to its high depth. Multiple platforms variant calling tools are available for TS, making it excruciating researchers choose. Therefore, benchmarking study across different pipelines TS is imperative. In this study, we performed a of Reference OncoSpan FFPE (HD832) sample enriched TSO500 panel using four commercially sequencers, analyzed the...
It is difficult to diagnose brucellar spondylitis because of its nonspecific clinical, radiological, and histological characteristics. This study aimed determine whether real-time polymerase chain reaction (PCR) using formalin-fixed paraffin-embedded (FFPE) tissues was superior conventional serum-based methods for diagnosing spondylitis. retrospective included 31 patients with brucellosis a control group 20 people no history or exposure Brucella spp. Samples from all were evaluated Giemsa...
Whole genome bisulfite sequencing (WGBS), possesses the aptitude to dissect methylation status at nucleotide-level resolution of 5-methylcytosine (5-mC) on a genome-wide scale. It is powerful technique for epigenome in various cell types, and tissues. As recently established next-generation (NGS) platform, GenoLab M promising alternative platform. However, its comprehensive evaluation WGBS has not been reported. We sequenced two bisulfite-converted mammal DNA this research using our NovaSeq...
Citrullus ecirrhosus, rehmii, and naudinianus are three important related wild species of watermelon in the genus Citrullus, their morphological differences clear, however, chloroplast genome remain unknown. This study is first to assemble, analyze, publish complete genomes C. naudinianus. A comparative analysis was then conducted among seven extant species, results demonstrated that average sizes 157,005 bp, a total 130-133 annotated genes were identified, including 8 rRNA, 37 tRNA 85-88...