A5108003957- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Animal Genetics and Reproduction
- SARS-CoV-2 detection and testing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Extracellular vesicles in disease
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Alzheimer's disease research and treatments
- Genetic Mapping and Diversity in Plants and Animals
- Epigenetics and DNA Methylation
- Nanoplatforms for cancer theranostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Advanced Nanomaterials in Catalysis
- Neurogenetic and Muscular Disorders Research
- MicroRNA in disease regulation
National University of Singapore
2018-2024
Institute of Molecular and Cell Biology
2018-2021
Agency for Science, Technology and Research
2018-2021
Washington University in St. Louis
2015-2016
Abstract Despite intense interests in developing blood measurements of Alzheimer’s disease (AD), the progress has been confounded by limited sensitivity and poor correlation to brain pathology. Here, we present a dedicated analytical platform for measuring different populations circulating amyloid β (Aβ) proteins – exosome-bound vs. unbound directly from blood. The technology, termed mplified p lasmonic ex osome (APEX), leverages situ enzymatic conversion localized optical deposits...
Abstract Rapid, visual detection of pathogen nucleic acids has broad applications in infection management. Here we present a modular platform, termed enzyme-assisted nanocomplexes for identification (enVision). The system consists an integrated circuit enzyme–DNA nanostructures, which function as independent recognition and signaling elements, direct versatile from infected cells. built-in enzymatic cascades produce rapid color readout the naked eye; assay is thus fast (<2 h), sensitive...
Exosomes are nanoscale vesicles distinguished by characteristic biophysical and biomolecular features; current analytical approaches, however, remain univariate. Here, we develop a dedicated platform for multiparametric exosome analysis-through simultaneous evaluation of the same vesicles-directly in clinical biofluids. Termed templated plasmonics exosomes, technology leverages situ growth gold nanoshells on to achieve multiselectivity. For selectivity, nanoshell formation is tuned...
Despite the importance of nucleic acid testing in managing COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct sensitive viral RNA targets native clinical samples. The technology, termed catalytic amplification by transition-state switch (CATCH), leverages DNA-enzyme hybrid complexes form switch. By ratiometric tuning its constituents, multicomponent is prepared...
Motor neuron diversification and regionalization are important hallmarks of spinal cord development rely on fine spatiotemporal release molecular cues. Here, we present a dedicated platform to engineer complex profiles for directed neuronal differentiation. Methods: The technology, termed microhexagon interlace generation versatile gradients (microHIVE), leverages an interlocking honeycomb lattice microstructures dynamically pattern at high spatial resolution. By packing the microhexagons as...
Accessible and adaptable nucleic acid diagnostics remains a critical challenge in managing the evolving COVID-19 pandemic. Here, an integrated molecular nanotechnology that enables direct programmable detection of SARS-CoV-2 RNA targets native patient specimens is reported. Termed synergistic coupling responsive equilibrium enzymatic network (SCREEN), technology leverages tunable, catalytic nanostructures to establish interconnected, collaborative architecture. SCREEN mimics extraordinary...
Summary Despite its great potential, high‐throughput functional genomic data are rarely integrated and applied to characterizing the basis of fertility. We obtained reprocessed over 30 genomics datasets from human mouse germ cells perform genome‐wide prediction genes underlying various reproductive phenotypes in both species. Genes involved male fertility easier predict than their female analogs. Of multiple types examined, protein–protein interactions by far most informative for gene...
Spermatozoa are one of the few mammalian cell types that cannot be fully derived in vitro, severely limiting application modern genomic techniques to study germ biology. The current gold standard approach characterizing single-gene knockout mice is slow as generation each mutant line can take 6–9 months. Here, we describe an vivo rapid functional screening germline genes based on a new nonsurgical, nonviral transfection method deliver nucleic acids into testicular cells. By coupling...
Programmable Detection of SarS-coV-2In article number 2101155, Huilin Shao and co-workers report the collaborative coupling enzyme-DNA nanostructures to establish multi-enzyme catalysis.Through programmable favorable equilibrium architecture, technology achieves direct sensitive detection SARS-CoV-2 RNA in native patient samples, within 30 minutes at room temperature.
Abstract Spermatozoa are one of the few mammalian cells types that cannot be fully derived in vitro , severely limiting application modern genomic techniques to study germ cell biology. The current gold standard approach characterizing single gene knockout mice is slow as generation each mutant line can take 6-9 months. Here, we describe an vivo rapid functional screening germline genes based on a new non-surgical, non-viral transfection method deliver nucleic acids into testicular cells. By...