A5044038946- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- COVID-19 Clinical Research Studies
- Diagnosis and treatment of tuberculosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Infections and Vaccines
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- SARS-CoV-2 detection and testing
- Immune cells in cancer
- SARS-CoV-2 and COVID-19 Research
- Embedded Systems and FPGA Design
- Immune responses and vaccinations
- Clinical practice guidelines implementation
- Antibiotics Pharmacokinetics and Efficacy
- Long-Term Effects of COVID-19
- COVID-19 epidemiological studies
- Health Systems, Economic Evaluations, Quality of Life
- Cardiac tumors and thrombi
- Peptidase Inhibition and Analysis
- Forest, Soil, and Plant Ecology in China
- Inflammatory Biomarkers in Disease Prognosis
National University of Singapore
2009-2025
National University Hospital
2008-2025
Chinese General Hospital and Medical Center
2025
National University Health System
2009-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2023
Istituti Clinici Scientifici Maugeri
2023
Başkent University Hospital
2023
Universitat de Barcelona
2023
Barcelona Institute for Global Health
2023
European Respiratory Society
2023
Tuberculosis (TB), a chronic infectious disease of global importance, is facing the emergence drug-resistant strains with few new drugs to treat infection. Pulmonary cavitation, hallmark established disease, associated very high bacillary burden. Cavitation may lead delayed sputum culture conversion, drug resistance, and transmission The host immunological reaction Mycobacterium tuberculosis implicated in driving development TB cavities. characterized by matrix-degrading phenotype which...
Pulmonary cavities, the hallmark of tuberculosis (TB), are characterized by high mycobacterial load and perpetuate spread M. tuberculosis. The mechanism matrix destruction resulting in cavitation is not well defined. Neutrophils emerging as key mediators TB immunopathology their influx associated with poor outcomes. We investigated neutrophil-dependent mechanisms involved TB-associated using a cellular model, cohort 108 patients, separate patient lung biopsies. Neutrophil-derived...
Abstract Coronavirus disease has disrupted tuberculosis services globally. Data from 33 centers in 16 countries on 5 continents showed that attendance at was lower during the first 4 months of pandemic 2020 than for same period 2019. Resources are needed to ensure care continuity pandemic.
BACKGROUND: Increasing evidence suggests that post-TB lung disease (PTLD) causes significant morbidity and mortality. The aim of these clinical standards is to provide guidance on the assessment management PTLD implementation pulmonary rehabilitation (PR).METHODS: A panel global experts in field TB care PR was identified; 62 participated a Delphi process. 5-point Likert scale used score initial ideas for after several rounds revision document approved (with 100% agreement).RESULTS: Five were...
Tuberculosis (TB) preventive therapy (TPT) decreases the risk of developing TB disease and its associated morbidity mortality. The aim these clinical standards is to guide assessment, management infection (TBI) implementation TPT.
BACKGROUND: Optimal drug dosing is important to ensure adequate response treatment, prevent development of resistance and reduce toxicity. The aim these clinical standards provide guidance on 'best practice´ for management TB drugs.METHODS: A panel 57 global experts in the fields microbiology, pharmacology care were identified; 51 participated a Delphi process. 5-point Likert scale was used score draft standards. final document represents broad consensus approved by all participants.RESULTS:...
ObjectivesAlthough evidence is growing on the overall impact of COVID-19 pandemic tuberculosis (TB) services, global studies based national data are needed to better quantify extent and countries' preparedness tackle two diseases. The aim this study was compare number people with new diagnoses or recurrence TB disease, drug-resistant (DR)-TB, deaths in 2020 vs 2019 11 countries Europe, Northern America, Australia.MethodsTB managers directors reference centers selected provided agreed-upon...
ABSTRACT Background and objective: Patients with community‐acquired Acinetobacter baumannii (AB) pneumonia have been reported from subtropical countries. We investigated the epidemiology, clinical microbiological characteristics of (CAP) due to AB in Singapore. Method: A retrospective case series was performed over a 21‐month period at two institutions. Results: From 1 January 2007 30 September 2008, eight patients were diagnosed CAP AB. Seven had bacteraemia five sputum culture‐positive....
Abstract The importance of neutrophils in the pathology tuberculosis (TB) has been recently established. We demonstrated that TB lesions man are hypoxic, but how hypoxia influence lung tissue damage is unknown. investigated effect on neutrophil-derived enzymes and destruction TB. Human were stimulated with M . (M tb) or conditioned media from tb -infected monocytes (CoMTB). Neutrophil matrix metalloproteinase-8/-9 elastase secretion analysed by luminex array gelatin zymography, gene...
BACKGROUND. Matrix metalloproteinases (MMPs) are key regulators of tissue destruction in tuberculosis (TB) and may be targets for host-directed therapy. We conducted a phase II double-blind, randomized, controlled trial investigating doxycycline, licensed broad-spectrum MMP inhibitor, patients with pulmonary TB.
The Western Pacific Region has one of the fastest-growing populations older adults (≥ 65 years) globally, among whom tuberculosis (TB) poses a particular concern. This study reports country case studies from China, Japan, Republic Korea, and Singapore reflecting on their experiences in managing TB adults.Across all four countries, notification incidence rates were highest adults, but clinical public health guidance focused this population was limited. Individual illustrated range practices...
Adverse effects (AE) to TB treatment cause morbidity, mortality and interruption. The aim of these clinical standards is encourage best practise for the diagnosis management AE.
Central nervous system tuberculosis (CNS TB) has a high mortality and morbidity associated with severe inflammation. The blood-brain barrier (BBB) protects the brain from inflammation but mechanisms causing BBB damage in CNS TB are uncharacterized. We demonstrate that Mycobacterium (Mtb) causes breakdown of type IV collagen decreases tight junction protein (TJP) expression co-culture model BBB. This increases permeability, surface endothelial adhesion molecules leukocyte transmigration. TJP...
Effective containment of the COVID-19 pandemic requires rapid and accurate detection pathogen. Polymerase chain reaction (PCR) remains gold standard for confirmation. In this article, we report performance a cost-effective modular microfluidic reverse transcription (RT)-PCR RT-loop mediated isothermal amplification (RT-LAMP) platform, Epidax®, point-of-care testing confirmation SARS-CoV-2. This platform is versatile can be reconfigured either screening using endpoint RT-PCR or RT-LAMP tests...
Central nervous system tuberculosis (CNS-TB) may be fatal even with treatment. Neutrophils are the key mediators of TB immunopathology, and raised CSF matrix metalloproteinase-9 (MMP-9) which correlates to neutrophil count in CNS-TB is associated neurological deficit death. The mechanisms by neutrophils drive TB-associated CNS destruction not clearly defined. Human brain biopsies histologically proven were stained for neutrophils, elastase, MMP-9. Neutrophil MMP-9 secretion gene expression...
The aim of these clinical standards is to provide guidance on 'best practice´ for diagnosis, treatment and management drug-susceptible pulmonary TB (PTB).