A5006033448- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
- Nanoparticle-Based Drug Delivery
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Chromatography
- Nitric Oxide and Endothelin Effects
- Cancer therapeutics and mechanisms
- Virus-based gene therapy research
- Click Chemistry and Applications
- Carbohydrate Chemistry and Synthesis
- Antimicrobial Peptides and Activities
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Plant-based Medicinal Research
- Chemical synthesis and alkaloids
- Asymmetric Synthesis and Catalysis
- Biopolymer Synthesis and Applications
- Analytical Methods in Pharmaceuticals
- Protein Hydrolysis and Bioactive Peptides
Southeast University
2021-2024
State Key Laboratory of Digital Medical Engineering
2021-2024
Hebei University
2023
Zhejiang University
2022
State Key Laboratory of Rice Biology
2022
Institute of Pharmacology
2004-2019
Shenyang Pharmaceutical University
2010-2019
Ningxia Medical University
2019
Dalian University
2014
Dalian University of Technology
2014
The orderly arrangement of nanomaterials' tiny units at the nanometer-scale accounts for a substantial part their remarkable properties. Maintaining this orderness and meanwhile endowing nanomaterials with highly precise free-designed 3D micro architectures will open an exciting prospect various novel applications. In paper, we developed sacrificial-scaffold-mediated two-photon lithography (TPL) strategy that enables fabrication complex colloidal crystal microstructures orderly-arranged...
A multifunctional nanocarrier with multilayer core–shell architecture was prepared by alkaline coprecipitation of ferric and ferrous ions in the presence a triblock copolymer, methoxy poly(ethylene glycol)-block-poly(methacrylic acid)-block-poly(glycerol monomethacrylate) (denoted MPEG-b-PMAA-b-PGMA), aqueous solution. The core is superparamagnetic Fe3O4 nanoparticle, on which PGMA block copolymer attached. PMAA forms inner shell MPEG outermost shell. anticancer agent adriamycin (ADR), as...
Peptide amphiphiles are capable of self-assembly into a diverse array nanostructures including ribbons, tubes, and vesicles. However, the ability to select morphology resulting structure is not well developed. We examined influence systematic changes in number type hydrophobic hydrophilic amino acids on amphiphilic peptides. Variations self-assembled peptides form X6Kn (X = alanine, valine, or leucine; K lysine; n 1–5) investigated using combination transmission electron microscopy dynamic...
Peptide vectors offer a promising gene delivery approach because of their biocompatibility and ease functionalization. This article describes the design evaluation series multifunctional peptides abilities. The were composed cell-penetrating segment, stearyl moiety, cationic amphiphilic α-helical cysteine histidine residues. proton sponge effect residues at low pH conformation should improve endosomal escape. Inclusion d-type amino acids proteolytic stability. conformation, particle size...
Liquid surface curvature variations in microplate wells due to different liquid tension cause significant signal change spectroscopic measurement using a plate reader with vertical detecting light beam. The signals have been quantitated and used develop method for facile surfactant critical micelle concentration determination.
The hexameric α-helical coiled-coil formed between the C-terminal and N-terminal heptad repeat (CHR NHR) regions of class I viral fusion proteins plays an important role in mediating cellular membranes provides a clear starting point for molecular mimicry that drives inhibitor design. Unfortunately, such peptide short region CHR Middle East respiratory syndrome coronavirus (MERS-CoV) spike protein has been thwarted by loss peptide's native conformation when taken out parent structure. Here,...
Class I enveloped viruses share similarities in their apparent use of a hexameric coiled-coil assembly to drive the merging virus and host cell membranes. Inhibition coiled coil-mediated interactions using bioactive peptides that replicate an α-helical chain from viral fusion machinery has significant antiviral potential. Here, we present construction series lipopeptides composed de novo heptad repeat sequence-based peptide plus hydrocarbon tail. Promisingly, constructs adopted stable...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA novel DNA duplex. A parallel-stranded helix with Hoogsteen base pairingKeliang Liu, H. Todd Miles, Joe Frazier, and V. SasisekharanCite this: Biochemistry 1993, 32, 44, 11802–11809Publication Date (Print):November 9, 1993Publication History Published online1 May 2002Published inissue 9 November 1993https://pubs.acs.org/doi/10.1021/bi00095a008https://doi.org/10.1021/bi00095a008research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of d(T)n.cntdot.d(A)n.cntdot.d(T)n: the DNA triple helix has B-form geometry with C2'-endo sugar puckerF. B. Howard, H. Todd Miles, Keliang Liu, J. Frazier, G. Raghunathan, and V. SasisekharanCite this: Biochemistry 1992, 31, 44, 10671–10677Publication Date (Print):November 1, 1992Publication History Published online1 May 2002Published inissue 1 November...
Nanocarriers with multilayer core-shell architecture were prepared by coating a superparamagnetic Fe(3)O(4) core triblock copolymer. The first block of the copolymer formed biocompatible outermost shell nanocarrier. second that contains amino groups and hydrophobic moiety inner shell. third bound tightly onto core. Chlorambucil (an anticancer agent) indomethacin anti-inflammation agent), each containing carboxyl group moiety, loaded into amino-group-containing combination ionic interactions....
Triterpene saponins are a major group of active components in natural products with nonspecific antiviral activities, while T20 peptide (enfuvirtide), which contains helix zone-binding domain (HBD), is gp41-specific HIV-1 fusion inhibitor. In this paper, we report the design, synthesis, and structure–activity relationship (SAR) hybrid molecules bioactive triterpene sapogenins were covalently attached to HBD-containing peptides via click chemistry. We found that either triterpenes or part...
Abstract Photonic crystal hydrogels (PCHs), with smart stimulus‐responsive abilities, have been widely exploited as colorimetric sensors for years. However, the current fabrication technologies are mostly applicable to produce PCHs simple geometries at sub‐millimeter scale, limiting introduction of structural design into PCH well accompanied advanced applications. This paper reports microfabrication three‐dimensional (3D) help supramolecular agarose a sacrificial scaffold by two‐photon...
Extended purine sequences on a DNA strand can lead to the formation of triplex in which third runs parallel strand. Triplex structures have been proposed play role gene expression and recombination also potential application as antisense inhibitors expression. studied solution by NMR, but hitherto resisted attempts at crystallization. Here, we report novel design sequences, allows first crystallographic study segment containing triplexes its junction with duplex. In 1.8 Å resolution...
Abstract Methoxypoly(ethylene glycol)–oligo(aspartic acid) (MPEG–Asp n ‐NH 2 , = 2–5) hybrid block copolymers were synthesized and used as stabilizers to prepare superparamagnetic Fe 3 O 4 nanoparticles with magnetite the inner core poly(ethylene glycol) hydrophilic outer shell. The aqueous dispersions of stable at pH 2–11 in 1 M NaCl solution, when repeat number, was or more. Transmission electron microscopy showed that nanoparticles, stabilized MPEG–Asp about 14 nm diameter. Magnetic...
Abstract Enfuvirtide (T20), is the first HIV fusion inhibitor approved for treatment of HIV/AIDS patients who fail to respond current antiretroviral drugs. However, its clinical application limited because short half-life, drug resistance and cross-reactivity with preexisting antibodies in HIV-infected patients. Using an artificial peptide strategy, we designed a non-native protein sequence, AP3, which exhibited potent antiviral activity against broad spectrum HIV-1 strains, including those...
To overcome barriers associated with gene delivery, a series of peptides consisting multifunctional fragments, including cationic amphiphilic α-helical antimicrobial peptide (AMP), cell penetrating (CPP), TAT, stearyl moiety, and cysteine residues, were designed synthesized for evaluation as non-viral vectors. TAT AMP segments utilized to mediate cellular uptake endosomal escape, respectively. Stearyl moieties provide an intramolecular hydrophobic environment promote AMPs form conformation...
Folate-functionalized magnetic fluids were prepared by direct chemisorption of a folate-tetra(ethylene glycol)-poly(glycerol monoacrylate) (FA-TEG-PGA) conjugate on Fe3O4nanoparticles. In the fluids, PGA block FA-TEG-PGA was chemisorbed onto Fe3O4nanoparticle surface through its 1,2-diol groups, while TEG chain conjugated with FA extended into water matrix. Characterization transmission electron microscopy, X-ray diffraction and vibrating sample magnetometer indicated that pure Fe3O4...