A5038645850- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Protein Kinase Regulation and GTPase Signaling
- Signaling Pathways in Disease
- Cellular transport and secretion
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- Cardiomyopathy and Myosin Studies
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- COVID-19 Clinical Research Studies
- Cancer Cells and Metastasis
- Chemical Reactions and Isotopes
- Pluripotent Stem Cells Research
- Graphene and Nanomaterials Applications
- Sphingolipid Metabolism and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Endoplasmic Reticulum Stress and Disease
- COVID-19 and healthcare impacts
- RNA modifications and cancer
- Infectious Aortic and Vascular Conditions
- Periodontal Regeneration and Treatments
- Hospitality and Tourism Education
- Cancer-related molecular mechanisms research
- Tourism, Volunteerism, and Development
California Northstate University
2024
Oroville Hospital
2022
Yale University
2015-2020
Massachusetts College of Pharmacy and Health Sciences
2020
University of the Incarnate Word
2017
The Geneva Foundation
2017
UConn Health
2010-2015
Inserm
2012
Centre National de la Recherche Scientifique
2012
The human steroid receptor RNA activator (SRA) gene encodes both noncoding RNAs (ncRNAs) and protein-generating isoforms. In reporter assays, SRA ncRNA enhances nuclear myogenic differentiation 1 (MyoD)-mediated transcription but also participates in specific corepressor complexes, serving as a distinct scaffold. That levels might affect some biological functions, such proliferation, apoptosis, steroidogenesis, myogenesis, has been reported. However, the breadth of endogenous target genes...
Rationale: Cardiac myocyte hypertrophy is the main compensatory response to chronic stress on heart. p90 ribosomal S6 kinase (RSK) family members are effectors for extracellular signal-regulated kinases that induce growth. Although increased RSK activity has been observed in stressed myocytes, functions of individual have remained poorly defined, despite being potential therapeutic targets cardiac disease. Objective: To demonstrate type 3 (RSK3) required hypertrophy. Methods and Results:...
The development of an alternative source for donor lungs would change the paradigm lung transplantation. Recent studies have demonstrated potential feasibility using decellularized as scaffolds tissue regeneration and subsequent implantation. However, finding a reliable cell ability to scale up recellularization scaffold still remain significant challenges. To explore possibility human from stem cells in vitro, populations progenitor were generated iPSCs. producing engineered cells, we...
Adipose-derived mesenchymal cells (ACs) and bone marrow-derived (BMCs) have been widely used for regeneration can be seeded on a variety of rigid scaffolds. However, to date, direct comparison (MC) harvested from different tissues the same donor cultured in identical osteogenic conditions has not investigated. Indeed, it is unclear whether or fat-derived MC possess intrinsic differences bone-forming capabilities, since within-patient comparisons previously done. This study aims at comparing...
Here we report the creation of a novel tracheal construct in form an engineered, acellular tissue-stent biocomposite trachea (TSBT). Allogeneic or xenogeneic smooth muscle cells are cultured on polyglycolic acid polymer-metal stent scaffold leading to formation tissue comprising cells, their deposited collagenous matrix, and material. Thorough decellularization then produces final tubular construct. Engineered TSBTs were tested as end-to-end replacements 11 rats 3 nonhuman primates. Over...
To date, efforts to generate engineered lung tissue capable of long-term function have been limited by incomplete barrier formation between air and blood thrombosis the microvasculature upon exposure collagens within decellularized scaffold. Improved resistance both depend recapitulation a confluent monolayer functional endothelium throughout pulmonary vasculature. This manuscript describes novel strategies increase cell coverage vascular surface area, compared previous reports in our lab...
The ubiquitously expressed and highly promiscuous protein phosphatase 1 (PP1) regulates many cellular processes. Targeting PP1 to specific locations within the cell allows for regulation of by conferring substrate specificity. In present study, we identified AKAP79 as a novel regulatory subunit. Immunoprecipitaiton AKAP from rat brain extract found that catalytic subunit copurified with anchoring protein. This is direct interaction, demonstrated pulldown experiments using purified proteins....
In cellular, tissue-level or whole organ bioreactors, the level of dissolved oxygen is one most important factors requiring control. Hypoxic environments may lead to cellular apoptosis, while hyperoxic damage dedifferentiation, both resulting in loss overall tissue function. This manuscript describes creation, characterization and validation a bioreactor system that can control delivery based on real-time metabolic demand cultured lung tissue. A mathematical model describing predicting gas...
The regulation of kinases by scaffolding proteins greatly contributes to the fidelity signal transduction. In present study, we explored an interaction between ubiquitous enzyme PKC (protein kinase C) and protein AKAP7 (A-kinase-anchoring 7). Using biochemistry surface plasmon resonance approaches, demonstrate that both AKAP7γ AKAP7α are capable high-affinity interactions with multiple isoenzymes PKC. Furthermore, this is achieved via multi-site binding on proteins. FRET (fluorescence energy...
A-kinase anchoring proteins (AKAPs) constitute a family of scaffolding that contribute to spatiotemporal regulation PKA-mediated phosphorylation events. In particular, AKAP7 is alternatively spliced participates in cardiac calcium dynamics. Here, we demonstrate via pull-down from transfected cells and by direct protein-protein association AKAP7γ self-associates. Self-association appears be an isoform specific phenomenon, as AKAP7α did not associate with itself or AKAP7γ. However, AKAP7δ,...
Receptor Expressed in Lymphoid Tissues (RELT) is a TNFRSF member that has two paralogs, RELL1 and RELL2; the three proteins are collectively referred to as RELT family members (RELTfms). We sought evaluate expression cancerous cells by using real-time PCR, western blotting, flow cytometry, immunohistochemistry (IHC). The mechanism of RELT-induced cell death was assessed luciferase assays, morphology staining. localization detected through immunofluorescence co-immunoprecipitation used test...