A5100392677- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Neuroblastoma Research and Treatments
- Metabolism and Genetic Disorders
- Spinal Hematomas and Complications
- Erythrocyte Function and Pathophysiology
- Spinal Fractures and Fixation Techniques
- Antimicrobial Peptides and Activities
- Immune Cell Function and Interaction
- Vascular Malformations Diagnosis and Treatment
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Influenza Virus Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Intracranial Aneurysms: Treatment and Complications
- Phagocytosis and Immune Regulation
- Neurofibromatosis and Schwannoma Cases
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Cell death mechanisms and regulation
- Endoplasmic Reticulum Stress and Disease
- Acupuncture Treatment Research Studies
- Invertebrate Immune Response Mechanisms
- Lipid metabolism and biosynthesis
- Nitric Oxide and Endothelin Effects
- Insect Utilization and Effects
Rutgers New Jersey Medical School
2007-2024
Rutgers, The State University of New Jersey
2007-2024
Karolinska Institutet
2009-2022
Tianjin Medical University
2011-2022
Guangdong Provincial Hospital of Traditional Chinese Medicine
2019-2022
Beijing Shijitan Hospital
2022
Karolinska University Hospital
2008-2022
Tianjin Medical University General Hospital
2016-2022
China University of Geosciences
2022
Capital University
2022
Mitochondrial dynamics is a fundamental cellular process and recruitment of Drp1 to mitochondria an essential step in mitochondrial fission. Mff MIEF1/2 (MiD51/49) serve as key receptors for mammals. However, if how these work together fission poorly understood. Here we show that MIEFs interact with both on the surface adaptors linking trimeric Drp1-MIEF-Mff complex. Thus, can regulate interaction between Mff, also Mff-induced accumulation mitochondria. It shown loss endogenous severely...
Recruitment of the GTPase dynamin-related protein 1 (Drp1) to mitochondria is a central step required for mitochondrial fission. Reversible Drp1 phosphorylation has been implicated in regulation this process, but whether at Ser-637 determines its subcellular localization and fission activity remains be fully elucidated. Here, using HEK 293T cells immunofluorescence, immunoblotting, RNAi, fractionation, co-immunoprecipitation assays, CRISPR/Cas9 genome editing, we show that phosphorylated...
NADPH oxidases (Noxes) produce ROS that regulate cell growth and death. NOX4 expression in cardiomyocytes (CMs) plays an important role cardiac remodeling injury, but the posttranslational mechanisms modulate this enzyme are poorly understood. Here, we determined FYN, a Src family tyrosine kinase, interacts with C-terminal domain of NOX4. FYN colocalized perinuclear mitochondria, ER, nuclear fractions CMs, negatively regulated NOX4-induced O2– production apoptosis CMs. Mechanistically, found...
Macrophages detect pathogen infection via the activation of their plasma membrane-bound Toll-like receptor proteins (TLRs). The heterotypic interaction between Toll/interleukin-1 (TIR) domains TLRs and adaptor proteins, like Myeloid differentiation primary response gene 88 (MyD88), is first intracellular step in signaling pathway mammalian innate immune response. hetero-oligomerization TIRs brings about transcription factor NF-κB, which regulates synthesis pro-inflammatory cytokines. Here,...
Although several proteins involved in mediating mitochondrial division have been reported mammals, the mechanism of fission machinery remains to be elucidated. Here, we identified a human nuclear gene (named MTGM) that encodes novel, small, integral inner-membrane protein and shows high expression both brain tumor cell lines tissues. The is evolutionarily highly conserved, its orthologs are 100% identical at amino acid level all analyzed mammalian species. product characterized by an unusual...
Several antimicrobial peptides (AMPs) belonging to the cecropin family have been identified from salivary glands of different black fly species, however, immunological functions for these molecules were poorly understood. A novel cecropin-like peptide (SibaCec) was purified using reverse phase high-performance liquid chromatography (RP-HPLC) Simulium bannaense. The amino acid sequence SibaCec determined by a combination method automated Edman degradation and cDNA sequencing. morphologic...
Abstract Background Mitochondrial dynamics is the result of a dynamic balance between fusion and fission events, which are driven via set mitochondria-shaping proteins. These proteins generally considered to be binary components either or machinery, but potential crosstalk machineries remains less explored. In present work, we analyzed roles mitochondrial elongation factors 1 2 (MIEF1/2), core machinery in mammals. Results We show that MIEFs besides their action regulate through direct...
Study Design. One hundred twenty-four patients with spinal dumbbell tumors were analyzed retrospectively using a new classification. Objective. To recommend novel classification of based on the characteristics surgical approach. Summary Background Data. Current classifications cervical tumor are excessively convoluted an impractical number variables or lack quantitative indicators for choice approach, and there few described in literature which related to thoracic lumbar tumors. An ideal...
Abstract BACKGROUND Amplification of the N‐ myc oncogene is associated with adverse outcomes in common childhood tumor, neuroblastoma. Because transforming properties Myc are related to its ability modulate gene expression, authors used cDNA microarrays identify potential target genes. METHODS Expression levels 4608 genes were analyzed a series neuroblastoma cell lines. Identical analyses performed panel medulloblastoma lines c‐Myc targets and determine extent which N‐Myc shared. Comparisons...
Four potent native human monoclonal antibodies (mAbs) targeting distinct epitopes on tetanus toxin (TeNT) are isolated with neutralization potency ranging from approximately 17 mg to 6 each that equivalent 250 IU of anti-TeNT immunoglobulin. TT0170 binds fragment B, and TT0069 TT0155 bind AB. mAb TT0067 C blocks the binding TeNT gangliosides. The co-crystal structure at a 2.0-Å resolution demonstrates directly occupies W pocket one receptor sites TeNT, resulting in blocking ganglioside...
This study investigated the surgical results of a single-stage posterolateral approach with arc incision, unilateral laminectomy, and costotransversectomy for management dumbbell tumors paraspinal thoracic spine. From January 2010 to March 2017, 14 patients or spine who underwent resection were followed up analyzed retrospectively. The operations performed using without any instrumentation. We reviewed scores clinical symptoms imaging results, including postoperative MRI reconstructed 3D-CT...
Neuroblastoma (NB), a childhood tumor derived from the sympathetic nervous system, presents with heterogeneous clinical behavior. While some tumors regress spontaneously without medical intervention, others are resistant to therapy, associated an aggressive phenotype. MYCN-amplification, frequently occurring in high-risk NB, is correlated undifferentiated phenotype and poor prognosis. Differentiation induction has been proposed as therapeutic approach for NB. We have previously shown that...
Colon cancer (CC) is one of the most common gastrointestinal malignancies. Effectiveness existing therapies limited. Immunotherapy a promising complementary treatment approach for CC. Major histocompatibility complex class I-related protein A and B (MICA/B) are ligands NK cells. Shedding MICA/B from surface tumor cells by cleavage at membrane proxial region in α3 structural domain immune evasion strategies leading to escape immunosurveillance. In this study, we generated panel monoclonal...
Long-chain free fatty acids (FFAs) accumulation and oxidative toxicity is a major cause for several pathological conditions. The mechanisms underlying FFA cytotoxicity remain elusive. Here we show that palmitic acid (PA), the most abundant in circulation, induces S403 phosphorylation of SQSTM1/p62 (sequestosome 1) its aggregation, which sequesters KEAP1 activates non-canonical SQSTM1-KEAP1-NFE2L2 antioxidant pathway. PA-induced SQSTM1 aggregation are dependent on K7-D69 hydrogen bond...
The Na+/Ca2+ exchanger (NCX) is a membrane antiporter that has been identified in the plasma membrane, inner of nuclear envelope and endoplasmic reticulum (ER). In humans, three genes have identified, encoding unique NCX proteins. Although extensively studied, NCX's sub-cellular localization mechanisms regulating activity different subtypes are still ambiguous. Here we investigated subcellular subtype 3 (NCX3) its impact on cell cycle. Two phenotypes, switching from one to other during...
Gentamicin is commonly used for effective treatment of severe Gram-negative bacterial infections. However, its use being increasingly restricted owing to the ototoxic effects attributed it. Gentamicin-induced ototoxicity thought be related with apoptosis induced by reactive oxygen species (ROS). In this study, we found a novel active peptide from Neptunea arthritica cumingii otoprotective and no significant embryotoxic effects. The combined application gentamicin helped sensory-hair cells...
The authors aimed to identify factors that influence neurological function after treatment in order facilitate clinician decision-making during of spinal cavernous malformation (SCM) and about when whether perform surgical intervention.
To explore the effect of electroacupuncture (EA) serum on expression myogenic differentiation antigen (Myod) and autophagy-related protein Beclin 1 in cultured muscle satellite cells rats under starvation conditions.The primary multifidus one male SD rat were isolated to obtain 3rd generation cells. The EA was got from received stimulation bilateral "Weizhong" (BL40, 2 Hz/10 Hz, mA, duration 20 min, once daily for 7 days). cell suspension (2×104/well) transferred each well a 96-well plate...
Single B cells-based antibody platforms offer an effective approach for the discovery of useful antibodies therapeutic or research purposes. Here we present a method screening equine immunoglobins F(ab)2, which offers potential advantage reacting with multiple epitopes on virus. Using influenza virus (EIV) as model, hemagglutinin (HA) trimer was constructed to bait cells in vaccinated horses. We screened 370 HA-specific from 1 × 10