A5050090359- Glioma Diagnosis and Treatment
- Fibroblast Growth Factor Research
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Bladder and Urothelial Cancer Treatments
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Angiogenesis and VEGF in Cancer
- Cancer Research and Treatments
- Microbial Natural Products and Biosynthesis
- Neuroblastoma Research and Treatments
- Phagocytosis and Immune Regulation
- RNA Interference and Gene Delivery
- Lung Cancer Research Studies
- interferon and immune responses
- Protein Degradation and Inhibitors
- Cancer, Lipids, and Metabolism
- Virus-based gene therapy research
- Renal and related cancers
- Nanoplatforms for cancer theranostics
- DNA and Nucleic Acid Chemistry
- Cancer-related molecular mechanisms research
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2020-2025
Broad Institute
2020-2025
Medical University of Vienna
2016-2025
Center for Cancer Research
2025
Comprehensive Cancer Center Vienna
2015-2024
Dana-Farber Cancer Institute
2020-2024
Harvard University
2020-2024
Comprehensive Blood & Cancer Center
2024
U-M Rogel Cancer Center
2024
Boston University
2023
Abstract Histone 3 lysine27-to-methionine (H3-K27M) mutations most frequently occur in diffuse midline gliomas (DMGs) of the childhood pons but are also increasingly recognized adults. Their potential heterogeneity at different ages and locations is vastly understudied. Here, through dissecting single-cell transcriptomic, epigenomic spatial architectures a comprehensive cohort patient H3-K27M DMGs, we delineate how age anatomical location shape glioma cell-intrinsic -extrinsic features light...
Diffuse hemispheric gliomas, H3G34R/V-mutant (DHG-H3G34), are lethal brain tumors lacking targeted therapies. They originate from interneuronal precursors; however, leveraging this origin for therapeutic insights remains unexplored. Here, we delineate a cellular hierarchy along the interneuron lineage development continuum, revealing that DHG-H3G34 mirror spatial patterns of progenitor streams surrounding nests, as seen during human development. Integrating these findings with genome-wide...
Abstract Globally decreased histone 3, lysine 27 tri-methylation (H3K27me3) is a hallmark of H3K27-altered diffuse midline gliomas (DMGs) and group-A posterior fossa ependymomas (PFAs). DMGs are largely characterized by lysine-to-methionine mutations in 3 at position (H3K27M). Most PFAs overexpress EZH inhibitory protein (EZHIP), which possesses region similarity to the mutant H3K27M. Both H3K27M EZHIP inhibit function polycomb repressive complex 2 (PRC2) responsible for H3K27me3 deposition....
Studying the intracellular distribution of pharmacological agents, including anticancer compounds, is central importance in biomedical research. It constitutes a prerequisite for better understanding molecular mechanisms underlying drug action and resistance development. Hyperactivated fibroblast growth factor receptors (FGFRs) constitute promising therapy target several types malignancies lung cancer. The clinically approved small-molecule FGFR inhibitor nintedanib exerts strong...
Metal-driven self-assembly afforded a multitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts, host-guest, and stimuli-responsive systems. However, the interest in biological SCCs is only starting to emerge thorough characterization their behavior milieus still lacking. Herein, we report on synthesis detailed in-cell tracking Pt
Collateral lethality occurs when loss of a gene/protein renders cancer cells dependent on its remaining paralog. Combining genome-scale CRISPR/Cas9 loss-of-function screens with RNA sequencing in over 900 cell lines, we found that cancers nervous system lineage, including adult and pediatric gliomas neuroblastomas, required the nuclear kinase vaccinia-related 1 (VRK1) for their survival vivo. VRK1 dependency was inversely correlated expression paralog VRK2. VRK2 knockout sensitized to loss,...
Abstract We have recently demonstrated that riboflavin ( Rf ) functions as unconventional bioorthogonal photocatalyst for the activation of Pt IV prodrugs. In this study, we show how combination light and with two prodrugs is a feasible strategy light-mediated pancreatic cancer cell death induction. Capan-1 cells, which high tolerance against photodynamic therapy, -mediated cisplatin carboplatin cis , trans -[Pt(NH 3 2 (Cl) (O CCH CH CO H) ] 1 (CBDCA)(O where CBDCA = cyclobutane...
Treatment with small-molecule inhibitors, guided by precision medicine has improved patient outcomes in multiple cancer types. However, these compounds are often not effective against central nervous system (CNS) tumors. The failure of approaches for CNS tumors is frequently attributed to the inability cross blood-brain barrier (BBB), which impedes intratumoral target engagement. This complicated fact that information on penetration CNS-tumor patients still very limited. Herein, we evaluated...
Pediatric high-grade gliomas (pHGGs) are among the most lethal childhood tumors. While therapeutic approaches were largely adapted from adult treatment regime, significant biological differences between pediatric and exist, which influence immune microenvironment may contribute to limited response current pHGG strategies. We provide a comprehensive transcriptomic analysis of landscape using single-cell RNA sequencing spatial transcriptomics. analyze matched malignant, myeloid, T cells...
Recently, in vitro anti-cancer properties of beauvericin, a fungal metabolite were shown various cancer cell lines. In this study, we assessed the specificity effect by comparing beauvericin cytotoxicity malignant versus non-malignant cells. Moreover, tested vivo anticancer effects treating BALB/c and CB-17/SCID mice bearing murine CT-26 or human KB-3-1-grafted tumors, respectively. Tumor size weight measured histological sections evaluated Ki-67 H/E staining as well...
Ependymomas (EPN) are central nervous system tumors comprising both aggressive and more benign molecular subtypes. However, therapy of the high-risk subtypes posterior fossa group A (PF-A) supratentorial RELA-fusion positive (ST-RELA) is limited to gross total resection radiotherapy, as effective systemic treatment concepts still lacking. We have recently described fibroblast growth factor receptors 1 3 (FGFR1/FGFR3) oncogenic drivers EPN. underlying mechanisms their potential therapeutic...
Abstract Posterior fossa type A (PF-EPN-A, PFA) ependymoma are aggressive tumors that mainly affect children and have a poor prognosis. Histopathology shows significant intratumoral heterogeneity, ranging from loose tissue to often sharply demarcated, extremely cell-dense tumor areas. To determine molecular differences in morphologically different areas understand their clinical significance, we analyzed 113 PF-EPN-A samples, including 40 corresponding relapse samples. Cell-dense ranged 0...
Glioblastoma (GBM) is characterized by a particularly invasive phenotype, supported oncogenic signals from the fibroblast growth factor (FGF)/ FGF receptor (FGFR) network. However, possible role of FGFR4 remained elusive so far. Several transcriptomic glioma datasets were analyzed. An extended panel primary surgical specimen-derived and immortalized GBM (stem)cell models original tumor tissues screened for expression. engineered wild-type dominant-negative overexpression investigated...
// Lisa Mayr 1 , Christine Pirker Daniela Lötsch Sushilla Van Schoonhoven Reinhard Windhager 2 Bernhard Englinger Walter Berger and Bernd Kubista Institute of Cancer Research Comprehensive Center, Department Medicine I, Medical University Vienna, 1090 Austria Orthopaedics, Correspondence to: Kubista, email: bernd.kubista@meduniwien.ac.at Keywords: CD44; osteosarcoma; metastasis; metastatic model; cilengitide Received: May 05, 2017 Accepted: November 28,...
// Bernhard Englinger 1 , Daniela Lötsch Christine Pirker Thomas Mohr Sushilla van Schoonhoven Bernd Boidol 2 Charles-Hugues Lardeau Melanie Spitzwieser 3 Pál Szabó 4 Petra Heffeter Irene Lang 5 Margit Cichna-Markl Bettina Grasl-Kraupp Brigitte Marian Michael Grusch Stefan Kubicek Gergely Szakács 1, 6 Walter Berger Institute of Cancer Research, Department Medicine I, Medical University Vienna, Austria CeMM Research Center for Molecular The Austrian Academy Sciences, Analytical Chemistry,...
Ponatinib is a small molecule multi‐tyrosine kinase inhibitor clinically approved for anticancer therapy. Molecular mechanisms by which cancer cells develop resistance against ponatinib are currently poorly understood. Likewise, intracellular drug dynamics, as well potential microenvironmental factors affecting the activity of this compound unknown. Cell/molecular biological and analytical chemistry methods were applied to investigate uptake kinetics/subcellular distribution, role lipid...
Purpose of review Bladder cancer incidence is on the rise, and until recently, there has been little to no change in treatment regimens over last 40 years. Hence, it imperative work strategies approaches untangle complexity intra- inter-tumour heterogeneity bladder with aim improving patient-specific care outcomes. The focus this therefore highlight novel targets, advances, therapy for patients. Recent findings success combining an antibody-drug conjugate (ADC) immunotherapy recently hailed...
Current risk stratification and treatment decision-making for bladder cancer informed by histopathology as well molecular diagnostics face limitations. This review summarizes recent advancements in single-cell spatial omics methodologies understanding biology their potential impact on development of novel therapeutic strategies.
Colorectal carcinoma (CRC) is the third most common cancer worldwide. Platinum-based anticancer compounds still constitute one mainstay of systemic CRC treatment despite limitations due to adverse effects and resistance development. Trabectedin has shown promising antitumor in CRC, however, again development may occur. In this study, we aimed develop strategies circumvent or even exploit acquired trabectedin novel regimens.Human HCT116 cells were selected for vitro characterised by cell...
Destruxins, secondary metabolites of entomopathogenic fungi, exert a wide variety interesting characteristics ranging from antiviral to anticancer effects. Although their mode action was evaluated previously, the molecular mechanisms resistance development are unknown. Hence, we have established destruxin-resistant sublines HCT116 colon carcinoma cells by selection with most prevalent derivatives, destruxin (dtx)A, dtxB and dtxE. Various cell biological techniques were applied elucidate...