A5024239119- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Protein Degradation and Inhibitors
- Exercise and Physiological Responses
- Sports Performance and Training
- Genetics and Physical Performance
- Chromatin Remodeling and Cancer
- Knee injuries and reconstruction techniques
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Radiation Dose and Imaging
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Bone health and osteoporosis research
- Protein Tyrosine Phosphatases
- Nuclear Structure and Function
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Muscle activation and electromyography studies
- Prostate Cancer Treatment and Research
- Nutrition and Health in Aging
- Melanoma and MAPK Pathways
- ATP Synthase and ATPases Research
Foghorn Therapeutics (United States)
2023-2025
Dana-Farber Cancer Institute
2017-2023
Broad Institute
2020-2023
Tufts University
2016-2023
Harvard University
2020-2022
Tufts Medical Center
2017-2022
University of Alabama at Birmingham
2004-2018
Geriatric Research Education and Clinical Center
2009-2011
National Student Clearinghouse Research Center
2007-2011
Birmingham VA Medical Center
2011
A present debate in muscle biology is whether myonuclear addition required during skeletal hypertrophy. We utilized K-means cluster analysis to classify 66 humans after 16 wk of knee extensor resistance training as extreme (Xtr, n = 17), modest (Mod, 32), or nonresponders (Non, 17) based on myofiber hypertrophy, which averaged 58, 28, and 0%, respectively (Bamman MM, Petrella JK, Kim JS, Mayhew DL, Cross JM. J Appl Physiol 102: 2232-2239, 2007). hypothesized that robust hypertrophy seen Xtr...
This article traces the history of peer review scientific publications, plotting development process from its inception to present-day application. We discuss merits and weaknesses, both perceived real, as well practicalities several major proposed changes system. It is our hope that readers will gain a better appreciation complexities and, when serving reviewers themselves, do so in manner enhance utility exercise. also propose an international on-line training program for accreditation...
While skeletal muscle protein accretion during resistance training (RT)-mediated myofiber hypertrophy is thought to result from upregulated translation initiation signaling, this concept based on responses a single bout of unaccustomed exercise (RE) with no measure across RT. Further, aging appears affect acute RE, but whether age differences in responsiveness persist RT leading impaired adaptation unclear. We therefore tested fractional synthesis rate (FSR) and Akt/mammalian target...
We applied K-means cluster analysis to test the hypothesis that muscle-specific factors known modulate protein synthesis and satellite cell activity would be differentially expressed during progressive resistance training (PRT, 16 wk) in 66 human subjects experiencing extreme, modest, failed myofiber hypertrophy. Muscle mRNA expression of IGF-I isoform Ea (IGF-IEa), mechanogrowth factor (MGF, IGF-IEc), myogenin, MyoD were assessed muscle biopsies collected at baseline (T1) 24 h after first...
The ability to control the timing and mode of host cell death plays a pivotal role in microbial infections. Many bacteria use toxins kill cells evade immune responses. Such are unknown Mycobacterium tuberculosis. Virulent M. tuberculosis strains induce necrotic macrophages by an obscure molecular mechanism. Here we show that protein Rv3903c (channel with necrosis-inducing toxin, CpnT) consists N-terminal channel domain is used for uptake nutrients across outer membrane secreted toxic...
The hypertrophic response to resistance training is generally attenuated with aging; yet the mechanisms regulating this phenomenon are largely unknown. Several studies date have shown blunted translational efficiency following acute exercise in older adults; however, effects on capacity (i.e., ribosome biogenesis) not been examined. Thus purpose of study was examine changes markers biogenesis an bout loading (RL; 9 sets × 10 repetitions knee extensions) younger (Y; n = 14; 39.2 ± 4.1 yr) and...
The purpose of this study was to identify signalling components known control mRNA translation initiation in skeletal muscle that are responsive mechanical load and may be partly responsible for myofibre hypertrophy. To accomplish this, we first utilized a human cluster model which skeletalmuscle samples fromsubjects with widely divergent hypertrophic responses resistance training were used the identification proteins associated degree We found 11 translational molecules examined, response...
Collateral lethality occurs when loss of a gene/protein renders cancer cells dependent on its remaining paralog. Combining genome-scale CRISPR/Cas9 loss-of-function screens with RNA sequencing in over 900 cell lines, we found that cancers nervous system lineage, including adult and pediatric gliomas neuroblastomas, required the nuclear kinase vaccinia-related 1 (VRK1) for their survival vivo. VRK1 dependency was inversely correlated expression paralog VRK2. VRK2 knockout sensitized to loss,...
We sought to determine the effect of varying rest intervals on leukocyte levels during heavy resistance exercise. Nine college men completed 2 exercise bouts 10 sets repetitions at 65% 1 repetition maximum (1RM) leg press with 1- (1MIN) or 3-minute (3MIN) intervals, respectively. Blood collected (PRE), immediately postexercise (POST), and 1.5 hours (1.5H) was analyzed for levels. Data were using a x 3 repeated measures analysis variance. A greater PRE-POST lymphocytosis (+83% vs. +16%, p =...
Purpose To determine if computed tomographic (CT) metrics of bone mineral density and muscle mass can improve the prediction noncancer death in men with localized prostate cancer. Materials Methods Institutional review board approval was obtained, waiver informed consent. All patients who underwent radiation therapy for cancer between 2001 2012 height, weight, past medical history documented CT that included L4-5 vertebral interspace were included. On a single axial section obtained at...
Abstract The paralog lysine acetyltransferases CREB binding protein (CBP) and E1A P300 (EP300) function as transcriptional coactivators that regulate diverse cellular programs. Mutations in CBP EP300 have been implicated the biology of various cancer types. bidirectional synthetic lethal relationship between these paralogs tumor cells highlights a therapeutic opportunity targeting selectively EP300-mutant cancers. We previously demonstrated our selective degraders potent antiproliferative...
The purpose of this study was to determine the effect long-term Cr supplementation on blood parameters reflecting liver and kidney function. Twenty-three members an NCAA Division II American football team (ages = 19–24 years) with at least 2 years strength training experience were divided into a monohydrate group (CrM, n 10) in which they voluntarily spontaneously ingested creatine, control ( 13) took no supplements. Individuals CrM averaged regular daily consumption 5 20 g (mean ± SD 13.9...
Sarcomere and cytoskeleton genes, or actomyosin regulate cell biology including mechanical stress, motility, division. While genes are recurrently dysregulated in cancers, their oncogenic roles have not been examined a lineage-specific fashion. In this report, we investigated dysregulation of nine sarcomeric cytoskeletal across 20 cancer lineages. We found that uterine cancers harbored the highest frequencies amplification overexpression gamma actin gene, ACTG1. Each four subtypes mixed...
Bladder cancer is a genetically heterogeneous disease, and novel therapeutic strategies are needed to expand treatment options improve clinical outcomes. Here, we identified unique subset of urothelial tumors with focal amplification the RAF1 (CRAF) kinase gene. RAF1-amplified had activation RAF/MEK/ERK signaling pathway exhibited luminal gene expression pattern. Genetic studies demonstrated that were dependent upon activity for survival, RAF1-activated cell lines patient-derived models...
Abstract E1A binding protein (EP300) and CREB (CBP) are paralog histone acetyltransferases involved in many cellular processes via their activity as transcriptional co-activators. Dysregulation of one or both proteins has been implicated various cancers, functional genomic screens have demonstrated a bidirectional synthetic lethal relationship between the two genes tumor cells. Due to high homology EP300 CBP, identifying chemical matter that selectively targets CBP proven challenging. Here,...
The purpose of this study was to evaluate the effectiveness repetitions fatigue (RTF) for estimating one-repetition maximum (1-RM) bench press performance in male high school athletes. Members athletic teams ( N = 213, age 16.3 ± 1.1 yrs, weight 79.9 16.7 kg) from four states were tested 1-RM and RTF after completing 4–6 weeks resistance training. A new equation use with athletes developed a random sample 180 participants; it appears have excellent predictive potential r 0.96, SEE 4.5...
Abstract Acute myeloid leukemia (AML) is a complex disease with multiple sub-types, each characterized by unique clinical and molecular features, driving the need to develop targeted therapies which exploit specific vulnerabilities. Bromodomain-containing protein 9 (BRD9) component of ncBAF chromatin remodeling complex, has been recently indicated as strong dependency in AML (Weisberg et al 2022). It shown that inhibitors BRD9 induce growth inhibition expression apoptotic makers cell lines...
We sought to determine the effect of varying rest intervals on leukocyte levels during heavy resistance exercise. Nine college men completed 2 exercise bouts 10 sets repetitions at 65% 1 repetition maximum (1RM) leg press with 1-(1MIN) or 3-minute (3MIN) intervals, respectively. Blood collected (PRE), immediately postexercise (POST), and 1.5 hours (1.5H) was analyzed for levels. Data were using a X 3 repeated measures analysis variance. A greater PRE-POST lymphocytosis (183% vs. 116%, p =...
The number of US fourth-year medical students applying to radiation oncology has decreased during the past few years. We conducted a survey examine factors that may be influencing decision pursue oncology.
Abstract CREB binding protein (CBP) and E1A (EP300) are paralog histone acetyltransferases involved in many cellular processes via their activity as transcription factor co-activators. Dysregulation of one or both proteins has been implicated various cancer types, functional genomic screens have revealed a bidirectional synthetic lethal relationship between these two paralogs tumor cells. Due to the high homology CBP EP300, identifying selective chemical matter that selectively disrupts...