A5075950170- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Genetics and Physical Performance
- Obesity, Physical Activity, Diet
- Extracellular vesicles in disease
- Nutrition and Health in Aging
- Cardiovascular and exercise physiology
- Physical Activity and Health
- Sports Performance and Training
- Thermoregulation and physiological responses
- Health and Lifestyle Studies
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Regulation of Appetite and Obesity
- Lipoproteins and Cardiovascular Health
- Body Composition Measurement Techniques
- Climate Change and Health Impacts
- GDF15 and Related Biomarkers
- Sirtuins and Resveratrol in Medicine
- Immune Response and Inflammation
- Cardiovascular Health and Disease Prevention
Pacific Northwest National Laboratory
2021-2025
Richland College
2024
Pacific Northwest University of Health Sciences
2022
Educational Service District 105
2021
University of Alabama at Birmingham
2016-2018
Central Washington University
2017
Brigham and Women's Hospital
2017
Children's National
2008-2016
George Washington University
2013-2016
University of Maryland, College Park
2010-2011
Regular exercise promotes whole-body health and prevents disease, but the underlying molecular mechanisms are incompletely understood
Abstract Subcutaneous white adipose tissue (scWAT) is a dynamic storage and secretory organ that regulates systemic homeostasis, yet the impact of endurance exercise training (ExT) sex on its molecular landscape not fully established. Utilizing an integrative multi-omics approach, leveraging data generated by Molecular Transducers Physical Activity Consortium (MoTrPAC), we show profound sexual dimorphism in scWAT sedentary rats response this to ExT. Specifically, females displays -omic...
Resistance exercise training (RT) is the most effective method for increasing skeletal muscle mass in older adults; however, amount of RT-induced growth highly variable between individuals. Recent evidence from our laboratory and others suggests ribosome biogenesis may be an important factor regulating hypertrophy, we hypothesized that extent hypertrophy at least partly regulated by biogenesis. To examine this, 42 adults underwent 4 wk RT aimed inducing knee extensors (e.g., 2 sets squat,...
Epigenomic regulation of the transcriptome by DNA methylation and posttranscriptional gene silencing miRNAs are potential environmental modulators skeletal muscle plasticity to chronic exercise in healthy diseased populations. We utilized networks connect exercise-induced differential miRNA with functional plasticity. Biopsies vastus lateralis were collected from middle-aged Polynesian men women morbid obesity (44 kg/m 2 ± 10) Type diabetes before following 16 wk resistance ( n = 9) or...
While regular physical activity is a cornerstone of health, wellness, and vitality, the impact endurance exercise training on molecular signaling within across tissues remains to be delineated. The Molecular Transducers Physical Activity Consortium (MoTrPAC) was established characterize networks underlying adaptive response exercise. Here, we describe studies undertaken by Preclinical Animal Sites Studies component MoTrPAC, in which sought develop implement standardized protocol large cohort...
Homozygosity for a premature stop codon (X) in the ACTN3 “sprinter” gene is common humans despite fact that it reduces muscle size, strength and power. Because of close relationship between skeletal function cardiometabolic health we examined influence R577X polymorphism over cardiovascular metabolic characteristics young adults (n = 98 males, n 102 females; 23 ± 4.2 years) from our Assessing Inherent Markers Metabolic syndrome Young (AIMMY) study. Both males females with RR vs XX genotype...
Lysine carbamylation is a non-enzymatic protein post-translational modification (PTM) that plays important roles in regulating enzymatic activity and the pathogenesis of diseases such as atherosclerosis, rheumatoid arthritis, uremia. The progress understanding biological systems has been delayed due to lack systematic assays study its functions. To aggravate this scenario, major artifact proteomics analysis given urea, which used during sample preparation, induces carbamylation. In addition,...
Abstract Despite the lungs being essential for ventilation and aerobic exercise capacity, conventionally are not thought to adapt training. Endurance is key pulmonary rehabilitation programs, which also displays sex-specific differences in therapeutic efficacy. Given molecular underpinnings of lung adaptations endurance uncharacterized, we used a multi-omics approach study sex 6-month-old Fischer 344 rats response an 8 week progressive treadmill training protocol. This was accomplished by...
The mechanisms by which exercise modulate liver metabolism, a central regulator of systemic are poorly understood. Leveraging data from MoTrPAC, we analyzed adaptations across 1, 2, 4, and 8 weeks in male female rats using multi-omic approaches. Female livers displayed progressive increase oxidative phosphorylation (OXPHOS) complexes (at the protein level), while showed an acetylation OXPHOS, TCA cycle, fatty acid oxidation enzymes. Exercise also enhanced cholesterol bile synthesis, reducing...
We initiated a pilot study to investigate the effects of 8 wks aerobic exercise training (ET) on insulin sensitivity and inflammatory markers in obese insulin-resistant minority adolescents. Eleven morbidly (BMI 41.4 ± 1.8 kg/m 2 ) adolescents were entered into supervised ET intervention (~180 min/wk at 40–55%VO 2Peak R [(VO − VO 2Rest )/VO ]). The (S I ), inflammation other metabolic syndrome features examined. Results: Insulin action improved response training, as indicated by ~37%...
Loss-of-function mutations in the dysferlin gene (DYSF) result a family of muscle disorders known collectively as dysferlinopathies. Dysferlin-deficient is characterized by inflammatory foci and macrophage infiltration with subsequent decline function. Whereas macrophages function to remove necrotic tissue acute injury, their prevalence chronic myopathy thought inhibit resolution regeneration. Two major classes macrophages, classical (M1) alternative (M2a), play distinct roles during injury...
What is the central question of this study? functional relevance OPN isoform expression in muscle pathology? main finding and its importance? The full-length human OPN-a most pro-inflammatory microenvironment, acting on macrophages myoblasts an RGD-integrin-dependent manner. upregulates tenascin-C (TNC), a known Toll-like receptor 4 (TLR4) agonist. Blocking TLR4 signalling inhibits effects OPN-a, suggesting that potential mechanism action by promoting TNC-TLR4 signalling. Although...
Abstract Regular exercise promotes whole-body health and prevents disease, yet the underlying molecular mechanisms throughout a whole organism are incompletely understood. Here, Molecular Transducers of Physical Activity Consortium (MoTrPAC) profiled temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome, immunome in blood, plasma, 18 solid tissues Rattus norvegicus over 8 weeks endurance training. The resulting data compendium...
<title>Abstract</title> The year of 2023 displayed the highest average global temperatures since it has been recorded—the duration and severity extreme heat are projected to increase. Rising represent a major public health threat, especially occupations exposed hot environments, such as construction agricultural workers, first responders. Despite efforts scientific community, there is still need characterize pathophysiological processes leading related illness develop biomarkers that can...
The year of 2023 displayed the highest average global temperatures since it has been recorded—the duration and severity extreme heat are projected to increase. Rising represent a major public health threat, especially occupations exposed hot environments, such as construction agricultural workers, first responders. Despite efforts scientific community, there is still need characterize pathophysiological processes leading related illness develop biomarkers that can predict its onset. Liquid...
ABSTRACT Introduction : Osteopontin (OPN) polymorphisms are associated with muscle size and modify disease progression in Duchenne muscular dystrophy (DMD). We hypothesized that OPN may share a molecular network myostatin (MSTN). Methods Studies were conducted the golden retriever (GRMD) mdx mouse models of DMD. Follow‐up in‐vitro studies employed myogenic cells treated recombinant (rm) or human (Hu) protein. Results: was increased MSTN decreased levels correlated inversely GRMD...
Abstract Extracellular vesicles (EVs) carry diverse biomolecules derived from their parental cells, making components excellent biomarker candidates. However, purifying EVs is a major hurdle in discovery since current methods require large amounts of samples, are time‐consuming and typically have poor reproducibility. Here we describe simple, fast, sensitive EV fractionation method using size exclusion chromatography (SEC) on fast protein liquid (FPLC) system. Our uses Superose 6 Increase...
The SORT1 locus was originally identified by genome-wide association studies of low-density lipoprotein cholesterol (LDL-C) in adults. Although the effect sizes this are relatively small, we hypothesized that a younger population would show greater genetic because fewer confounding variables. As such, investigated between and LDL-C group healthy young Subjects (n = 122, mean age 23.2 years) were recruited from University Calgary. Lipid measures genomic DNA collected peripheral blood after an...
Abstract Background The incidence of mucinous appendiceal adenocarcinomas (MAA) has increased over the past three decades. Advanced stage tumor diagnosis is likely attributable to non-specific findings. Here we describe advanced MAA presenting as inguinal ulcers, scrotal abscesses, and other nonspecific symptoms. To our knowledge, this first report pain with inflamed phlegmonous tissue abscess. Case presentation A 67-year-old male presented a rural facility complaining weight-loss, fatigue,...
Abstract Subcutaneous white adipose tissue (scWAT) is a dynamic storage and secretory organ that regulates systemic homeostasis, yet the impact of endurance exercise training sex on its molecular landscape has not been fully established. Utilizing an integrative multi-omics approach with data generated by Molecular Transducers Physical Activity Consortium (MoTrPAC), we identified profound sexual dimorphism in response rat scWAT to training. Despite similar cardiorespiratory improvements,...
Many, GM, Lutsch, A, Connors, KE, Shearer, J, Brown, HC, Ash, G, Pescatello, LS, Gordish-Dressman, H, Barfield, W, Dubis, Houmard, JA, Hoffman, EP, and Hittel, DS. Examination of lifestyle behaviors cardiometabolic risk factors in university students enrolled kinesiology degree programs. J Strength Cond Res 30(4): 1137–1146, 2016—Preventing physical inactivity weight gain during college is critical decreasing lifelong obesity associated disease risk. As such, we sought to compare between...