A5076234599- Periodontal Regeneration and Treatments
- Dental Research and COVID-19
- Pluripotent Stem Cells Research
- Dental Implant Techniques and Outcomes
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Semiconductor materials and interfaces
- Oral microbiology and periodontitis research
- Innovations in Medical Education
- Dental Radiography and Imaging
- Reconstructive Surgery and Microvascular Techniques
- Cellular Mechanics and Interactions
- Problem and Project Based Learning
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Oral and gingival health research
- Epigenetics and DNA Methylation
- Salivary Gland Disorders and Functions
- CRISPR and Genetic Engineering
- Electrodeposition and Electroless Coatings
- Metallic Glasses and Amorphous Alloys
- Thin-Film Transistor Technologies
- Dental Education, Practice, Research
- Dental Health and Care Utilization
Harvard University
1989-2024
University of Michigan
2024
Lynn University
2010-2023
McGill University
2018-2021
University of Washington Medical Center
2016
University of Washington
2013
Taiwan Adventist Hospital
2008-2010
University of Southern California
2010
Massachusetts General Hospital
2009
Brigham and Women's Hospital
2008
Renewal of stem cells differs from cancer cell growth in self-controlled division. The mir-302 microRNA (miRNA) family (mir-302s) is expressed most abundantly slow-growing human embryonic (ES) cells, and quickly decreases after differentiation proliferation. Therefore, mir-302s was investigated as one the key factors essential for maintenance ES renewal pluripotency this study. Pol-II-based intronic miRNA expression system used to transgenically transfect into several lines....
Global demethylation is required for early zygote development to establish stem cell pluripotency, yet our findings reiterate this epigenetic reprogramming event in somatic cells through ectopic introduction of mir-302 function. Here, we report that induced expression beyond 1.3-fold the concentration human embryonic (hES) H1 and H9 led hair follicle (hHFCs) pluripotent (iPS) cells. This mechanism functioned mir-302-targeted co-suppression four regulators, AOF2 (also known as KDM1 or LSD1),...
miR-302 is the major microRNA found in human embryonic stem cells and induced pluripotent cells, but its function has been unclear. In mice, there evidence that may silence p21Cip1 (CDKN1A) to promote cell proliferation, whereas studies reprogrammed suggested elevated expression inhibited cycle transit. Here, we clarify this difference, reporting simultaneously suppressed both cyclin E-CDK2 D-CDK4/6 pathways block>70% of G1-S transition. Concurrent silencing BMI-1, a cancer marker targeted...
Abstract Poorly immunogenic tumors, including triple negative breast cancers (TNBCs), remain resistant to current immunotherapies, due in part the difficulty of reprogramming highly immunosuppressive tumor microenvironment (TME). Here we show that peritumorally injected, macroporous alginate gels loaded with granulocyte-macrophage colony-stimulating factor (GM-CSF) for concentrating dendritic cells (DCs), CpG oligonucleotides, and a doxorubicin-iRGD conjugate enhance death cells, increase...
Abstract During the COVID‐19 pandemic, dental education community faced unprecedented challenges. In this commentary, we share perspectives of faculty clinicians, residents and students in academic institutions United States Canada. We discuss COVID‐19’s impact on various aspects dentistry including patient care, education, research raise key concerns regarding future post‐pandemic.
The exchange of mobile genetic elements (MGEs) facilitates the spread functional traits including antimicrobial resistance within bacterial communities. Tools to spatially map MGEs and identify their hosts in complex microbial communities are currently lacking, limiting our understanding this process. Here we combined single-molecule DNA fluorescence situ hybridization (FISH) with multiplexed ribosomal RNA-FISH enable simultaneous visualization both taxa. We mapped bacteriophage (AMR)...
Abstract Multicellular spheroids made of stem cells can act as building blocks that fuse to capture complex aspects native in vivo environments, but the effect hydrogel viscoelasticity on cell migration from and their fusion remains largely unknown. Here, we investigated behavior mesenchymal (MSC) using hydrogels with a similar elasticity different stress relaxation profiles. Fast relaxing (FR) matrices were found be significantly more permissive consequent MSC spheroids. Mechanistically,...
Alzheimer's disease (AD) is one of the most prevalent progressive neurodegenerative disorders and among leading causes mortality globally. AD characterized by continued cognitive behavioral impairments associated with memory loss, difficulty reasoning, language impairment. In parallel, periodontal (PD) a chronic disease, affecting in two adults, associations to various systemic disease. Recently, pre-clinical clinical studies have elucidated between PD AD. The recent evidence suggests...
Abstract Diseases and disorders of dental, oral, craniofacial (DOC) tissues represent a significant global health burden have been found to the greatest age‐standardized prevalence incidence all reported diseases worldwide. While application novel therapies has suggested address different types oral diseases, only limited number interventional regenerative improve clinical therapeutic outcomes. The lack in DOC tissue regeneration may be part attributed highly resource‐intensive translational...
Oral lichen planus (OLP) and recurrent aphthous stomatitis (RAS) are common chronic inflammatory conditions, manifesting as painful oral lesions that negatively affect patients’ quality of life. Current treatment approaches mainly palliative often ineffective due to inadequate contact time the therapeutic agent with lesions. Here, we developed Dental Tough Adhesive (DenTAl), a bioinspired adhesive patch robust mechanical properties, capable strong adhesion against diverse wet dynamically...
Cell-instructive biomaterials are an essential component in tissue engineering and regenerative medicine. In the past three decades since term “Tissue Engineering” was coined, researchers have made significant progress towards regenerating disease or damage tissues organs by combining innovations biomaterials, signaling molecules cell therapies. However, challenges persist including limitations properties of cell-instructive lack advanced manufacturing technologies for precise spatiotemporal...
Interdiffusion kinetics were measured using x-ray diffraction methods in epitaxial (001) and (111) Co/Pt multilayers which the chemical modulation wavelength was range 2.5–4.0 nm. Multilayers prepared by e-beam evaporation subsequently annealed vacuum at temperatures between 275 375 °C. The activation enthalpy of interdiffusion process this temperature estimated a novel approach for scaling nonlinear diffusion data. Activation enthalpies determined to be 1.1±0.2 0.8±0.2 eV, respectively. low...
We have performed in situ, energy-dispersive x-ray reflectivity measurements of damage layer formation and surface roughness thin films SiO2 on Si clean during H ion bombardment. The was analyzed using an optical multilayer model where the variable parameters are number layers, thickness density roughness. Room temperature bombardment at doses <5×1017/cm2 results a buried between oxide substrate; 300 eV produces very thin, low-density layer, while 1000 thicker with amorphous silicon....
An electrodeposition technique for making multilayered thin films, based on controlled deposition from two different solutions, is described. Ni/NiPx and NiPx/NiPy films with repeat lengths as low 20 Å have been prepared. They show up to three low-angle x-ray peaks corresponding the electron density modulation.
With current global trends in postgraduate education, graduate programmes must make evidence-based improvements to offer the best programme that aligns with student needs and prepare them for their future career prospects. The aim of this cross-sectional study was investigate postgraduation pathways MSc PhD students who graduated within past 15 years from McGill University Postgraduate Dental Research Program.An online questionnaire, composed 10 closed-ended format items, used covered...
Abstract Aim The aim of this study is to assess early wound healing expression local angiogenic biomarkers following connective tissue graft (CTG) at dental implant sites. Methods Twenty‐eight subjects with single implants exhibiting a soft dehiscence were included and randomly treated CTG, either coronally advanced flap (CAF) or tunnel technique (TUN). Peri‐implant crevicular fluid (PICF) was collected the midfacial midlingual aspect sites baseline 3, 7, 14, 30, 90 days after surgical...