Messenger RNA (mRNA) has emerged as a critical global technology that requires joint efforts from different entities to develop COVID-19 vaccine. However, the chemical properties of pose challenge in utilizing mRNA vaccine candidate. For instance, molecules are prone degradation, which negative impact on distribution among patients. In addition, little is known degradation individual bases molecule. Therefore, this study aims investigate whether hybrid deep learning can predict sequences....

To enhance our survey of Araucaria biflavonoid’s biological activity, cunninghamii Mudie dried leaf samples from West Java, Indonesia were extracted using acetone, then purified by Sephadex column and silica gel radial chromatography to give 3 known pure compounds: 7,4′,7″,4‴-tetra-O-methyl cupressuflavone, 7,4′,4‴-tri-O-methylrobustaflavone, 7,7″-di-O-methylamentoflavone. This is the first report 7,4′,4‴-tri-O-methylrobustaflavone 7,7″-di-O-methylamentoflavone isolation A. species. Among...

Objective: The immune system is a complex network of cells, tissues, and organs that work together to defend the body from attack by foreign organisms such as bacteria, viruses, parasites, fungi. Some natural medicines have been known activity immunomodulators. One them Sungkai leaf (Peronema canescens Jack). Methods: In this study, in silico testing was carried out between several active isolate compounds sungkai leaves proteins related system, namely Interleukin-6 (IL-6), Tumor Necrosis...

Snakebite envenomation remains a significant medical concern, particularly in tropical regions where venomous snakes such as Calloselasma rhodostoma and Trimeresurus insularis are prevalent. Both venoms known for their potent hemotoxic, myotoxic, inflammatory effects, yet differential impacts on systemic physiological pathways remain unclear. The aim of this study was to characterize the hematological, effects C. T. murine model explore influence factors insulin-like growth factor 1 (IGF-1),...

The Ti-6Al-4V metal alloy is an implantable material because of its strength, lightweightness, and corrosion resistance properties. Metal surfaces need to be modified improve resistance. Therefore, the study aims modify surface alloys form nanotube structures coat hydroxyapatite composite (HAp)-gelatin-PVA in alloys. Nanotubes were formed by anodizing methods ethylene glycol-H2O (9:1) solution containing 0.6% NH4F at 20V for 3 hours. result SEM showed that diameter hole about 30−40 nm it...

Lithium batteries development are triggered so many efforts in producing electronic devices due to its excellent performance as energy storage systems. One of the appealing points solid polymer electrolytes for developing solid-state lithium batteries. In this study, Solid with crosslinked treatment (SPE-C) were prepared from carboxymethyl cellulose-lithium perchlorate (CMC-LiClO4) and citric acid (CA) a crosslinker via solution casting method. All SPE-C membranes assembled into battery coin...

The aqueous sodium-ion battery is a promising alternative to the well-known lithium-ion owing large abundance of sodium ion resources. Although it safer than battery, voltage window narrower that thus limiting its practical implementation. Therefore, highly concentrated electrolyte required address this issue. In present work, effect salt concentration on transport properties water molecules investigated via theoretical analyses at quantum mechanical level. A molecular dynamics simulation...

Nanoparticles (NP) have been studied because of their unique physical and chemical properties compared with bulk counterparts. The ferrite colloids, magnetite (Fe3O4) are the main representatives magnetic nanoparticles (MNPs). These colloids characterized by a spinel crystal structure oxygen ions forming close-packed cubic lattice iron located at interstices. Fe3O4 has attracting in biomedical applications due to biocompatibility biodegradability. In this research, successfully synthetized...

Abstract Reuterin is a natural antifungal agent derived from certain strains of Limosilactobacillus reuteri . Our previous study revealed that 6 mM reuterin inhibited completely the conidial germination aflatoxigenic Aspergillus flavus This investigated potential molecular mechanism in inhibiting A. germination, which was pre‐assumed it correlated to inhibition some essential enzyme activity involved specifically 1,3‐β‐glucan synthase, chitin and catalases (catalase, bifunctional...

Objective: Andrographis paniculata has long been a traditional medicinal plant in Indonesia. This study is intended to evaluate the anti-pro-inflammatory cytokines of 98% ethanol extract A. by vitro and silico approaches. Inhibition pro-inflammatory also one therapies treating COVID-19. Methods: The molecular docking approach was utilized as first screening potential for suppression macrophage cell activation; an ADMET prediction test performed determine pharmacological, pharmacokinetic,...

Bioactive chemicals derived from Acacia auriculiformis and A. crassicarpa have the potential to be developed as sources of anti-cancer raw materials antioxidants, given that these plants are fast-growing species with medicinal capability. The in silico method was successful identifying bioactive for preliminary study. Using an approach, this work aimed identify most potent compounds inhibitors six cancer stress oxidative therapy-targeted proteins two distinct species. Seventeen out 37...

Uric acid, which causes gout, is the end product of purine catabolism, synthesized by xanthine oxidase, guanine deaminase, adenine nucleoside phosphorylase, and 5-nucleotidase II. Garlic contains bioactive compounds that have potential as antigout agents. fermentation to black garlic changes its components, may affect beneficial potential. This study aimed select types (Indonesian garlic) imported garlic, predict interaction between their five target proteins through an in silico approach a...

Horseshoe crab (Tachypleus gigas) amebocytes are useful biomedical components for endotoxin detection, and their growing needs purposes cause the horseshoe population to decline. Factor C synthesis via genetic engineering offers a solution replace natural crab's factor prevent its excessive harvest from nature. In response these concerns, this study aimed characterize amebocyte lysates protein modeling of T. gigas originated Banyuasin South Sumatra Estuary.

Acacia auriculiformis and A. crassicarpa can be used as non-timber forest products to produce therapeutic agents. To achieve this, it is essential identify the metabolite profile biological activity of these raw materials. In this study, a liquid chromatography high resolution mass spectrometry platform was metabolites in leaf, bark, heartwood extracts that were positively related antioxidant antiproliferative using vitro silico studies. extract showed significantly higher compared other...

Acid and Alkaline Hydrolysis Studies of Stevioside Rebaudioside AVenkata Sai Prakash Chaturvedula, Indra

Skin aging is a condition caused by reactive oxygen species (ROS) and advanced glycation end products (AGEs). Indonesian gingers (Zingiber officinale), which consists of Gajah (GG), Red (MM), Emprit (EE) ginger, are thought to produce anti-skin compounds through enzyme inhibition. The enzymes used in the molecular docking study were collagenase, hyaluronidase, elastase, tyrosinase. This aimed determine contained ginger rhizome ethanolic extracts using liquid chromatography–mass...

In silico designing of siRNA targeting PB 1 gene Influenza A virus and in vitro validation Bhawana Jain, Amita Om Prakash, Ajay Kr Singh, Tanushree Dangi, Mastan Kaleshwar P Singh

The escalating prevalence of diabetes mellitus (DM), a major global health threat and one the top 10 causes mortality, is worrying trend. Prolonged hyperglycemia in people with triggers oxidative stress, leading to accelerated tissue damage, increased levels free radicals, onset complications. Our initial exploration Physalis angulata L, using bioassay-guided isolation C2C12 insulin resistance monitoring, revealed compound’s ability increase cellular glucose uptake. Consequently, there an...

Hydroxyapatite (HA) was included mineral of apatite is a bioactive ceramic material and biocompatible, which has similarity with the nature bone chemical structure.The synthesis hydroxyapatite by using wet precipitation method consists calcination, hydration, HA synthesizes, thermal treatment.The solution 0.3 M (NH4)2.HPO4 dropped on suspension 0.5 Ca(OH)2 from tutut (Bellamya javanica) shells.While collagen extracted waste fish (Later calcarifer) scales following acid alkali extraction...