To determine the structure of triterpenoid isolated from avocado seeds and cytotoxic effect on MCF-7 HepG2 cells. The powder sample was macerated with ethanol, followed separation extract by column chromatography. target compound monitored thin layer chromatography plate reagent Lieberman–Buchard. characterized spectral analysis, mainly ultraviolet, infrared, liquid chromatography-mass spectroscopy their spectroscopic data those reported in literature were compared. In vitro activity...

Abstract Introduction The leaves of Annona muricata L., known as “soursop” or “ sirsak ” in Indonesia, are used traditionally for cancer treatment. However, the bioactive components remain largely unidentified. Objective This study untargeted liquid chromatography–tandem mass spectrometry (LC‐MS/MS)‐based metabolomics to identify potential cytotoxic compounds A. leaf extracts on MCF‐7 breast cells vitro. Methods were macerated with water, 99% ethanol, and aqueous mixtures containing 30%,...

To enhance our survey of Araucaria biflavonoid’s biological activity, cunninghamii Mudie dried leaf samples from West Java, Indonesia were extracted using acetone, then purified by Sephadex column and silica gel radial chromatography to give 3 known pure compounds: 7,4′,7″,4‴-tetra-O-methyl cupressuflavone, 7,4′,4‴-tri-O-methylrobustaflavone, 7,7″-di-O-methylamentoflavone. This is the first report 7,4′,4‴-tri-O-methylrobustaflavone 7,7″-di-O-methylamentoflavone isolation A. species. Among...

To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi). The leaf sample was powdered and macerated with methanol fractioned by liquid–liquid extraction using ethyl acetate. fraction chromatographed the isolates were identified for major component liquid chromatography-mass spectrometry antibacterial activity tested against S. Kirby–Bauer method. Subfraction 1 from acetate formed a yellowish solid m/z 272, as naringenin. naringenin-rich...

Uric acid, which causes gout, is the end product of purine catabolism, synthesized by xanthine oxidase, guanine deaminase, adenine nucleoside phosphorylase, and 5-nucleotidase II. Garlic contains bioactive compounds that have potential as antigout agents. fermentation to black garlic changes its components, may affect beneficial potential. This study aimed select types (Indonesian garlic) imported garlic, predict interaction between their five target proteins through an in silico approach a...

Indonesia is a tropical country with mega-biodiversity. Several medicinal plants locally have been recognized for their anti-inflammatory properties and are traditionally used to help treat respiratory diseases. Chronic obstructive pulmonary disease (COPD) one of the diseases known as high cause death globally, treatment efforts by using drugs. In developing alternative remedies COPD, this review summarizes potential Indonesian ingredients an activity develop COPD. Primarily, we focus on...

Hydroxychavicol is found in betel leaf at low concentration and reported to have antibacterial, anti-inflammatory, antioxidant, anticancer, antimutagenic activities. This study aimed synthesize hydroxychavicol from eugenol safrole. Isolation of clove oil by alkaline extraction method gave 71% yield, while the isolation safrole lawang method, followed purification using preparative thin layer chromatography, 7% yield. Eugenol demethylation demethylenation with AlCl3 reagent were successfully...

Senyawa asam laktat sangat dibutuhkan di dunia industri. Namun produksi dengan menggunakan mikrob masih bahan pangan sebagai substratnya. Alternatif substrat untuk pengganti penggunaan diperlukan Tetes tebu merupakan salah satu yang kaya akan sumber karbon dapat digunakan komponen media pertumbuhan bakteri. Ketersediaannya melimpah dan harganya murah. Tujuan penelitian ini adalah tetes alternatif bakteri Lactobacillus delbrueckii subsp. bulgaricus menghasilkan laktat. Langkah meliputi...

As a natural fiber and agricultural by-product, corn stalks ( Zea mays saccharata ) is considered as an alternative raw material to produce particleboard. Corn good source of lignocelluloses, renewable low cost. This research was aimed investigate the characteristics stalk particleboard with citric acid adhesive. study also evaluated bonding mechanism particle bonds between celluloses derived acid. The boards were manufactured under hot pressing temperature 200 o C for 10 min. concentration...

The traditional medicine has been used in Indonesia since the days of Ancient Mataram Kingdom (about 12 centuries ago). is rich medicinal plants. For this reason, it necessary to inform broader community regarding plants that have potential as antigout. prevalence gout range 1.6−13.6 per 100,000 people and will increase with age. There are 25 species Indonesian more than 50% xanthine oxidase (XO) enzyme inhibitory activity. XO responsible for catalyzing hypoxanthine then producing uric acid,...

Polylactic acid (PLA) is the most representative sustainable and bio-based polymer environmentally friendly that has a great potential to replace petroleum-based plastics. However, brittleness, low heat resistance, slow crystallization limit wide application of PLA. One strategies improve PLA properties by reinforcing with microfibrillated cellulose (MFC). Unfortunately, hydrophilic MFC make it difficult attain good dispersion in hydrophobic matrix. Therefore, modification was needed...

This study examined the supplementation effects of gliricidia leaves(GL, Gliricidia sepium), lerak fruit(LF, Sapindus rarak), or hibiscusleaves(HL, Hibiscusrosa-sinensis) on in vitro rumen fermentation and methanogenesis made a comparisonwith Napier grass (NG, Pennisetum purpureum) grass. In was designed according to randomized complete block design with four replications seven treatments: NG, GL, LF, HL, NG 70% + GL 30%, LF 30%and HL 30%. The generated data were subjected analysis variance...

Endophytic fungi have been known as potential source of bioactive compound, similar to their host. This study was conducted identify endophytic Diaporthe spp. isolated from Cinchona calisaya and explore in producing alkaloids, particularly quinine. A total 39 strains were identified using ITS1-5.8S-ITS2 EF1-α gene. Seventeen species name they represent eight i.e. D. cynaroidis, endophytica, ganjae, gardeniae, litchicola, phaseolorum, pseudomangiferae, rhoina. The re­maining 22 represents...

Liquid smoke has been used to extend the shelf life of food. However, its composition varies considerably depending on type raw materials and preparation precedure. derived from palm oil shell is potential due abundance byproduct sources in industry. This study thus aims prepare best fraction liquid that can beef meatballs at room temperature. The was redistilled 80, 90, 100°C as an ingredient production. gas chromatography-mass spectrometry identification showed there were no harmful...