A5052801713- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Occupational Health and Safety Research
- Atherosclerosis and Cardiovascular Diseases
- Gas Sensing Nanomaterials and Sensors
- COVID-19 Clinical Research Studies
- Amphibian and Reptile Biology
- Genetics, Aging, and Longevity in Model Organisms
- SARS-CoV-2 detection and testing
- Immune cells in cancer
- Optical Wireless Communication Technologies
- Quantum Information and Cryptography
- Traffic and Road Safety
- Aquaculture disease management and microbiota
- Advanced Algorithms and Applications
- Pluripotent Stem Cells Research
- Underwater Vehicles and Communication Systems
- Advanced Chemical Sensor Technologies
- Venomous Animal Envenomation and Studies
- Gene Regulatory Network Analysis
- Analytical Chemistry and Sensors
- Neuroscience and Neural Engineering
- Telomeres, Telomerase, and Senescence
- Transport and Economic Policies
- Innovation and Socioeconomic Development
University of Illinois Chicago
2021-2025
University of Illinois Urbana-Champaign
2021-2025
Illinois College
2021-2025
Chengdu University of Traditional Chinese Medicine
2024-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Guang’anmen Hospital
2025
Chinese Academy of Sciences
2020-2024
Air Force Medical University
2017-2024
Institute of Zoology
2020-2024
State Key Laboratory of Stem Cell and Reproductive Biology
2024
Abstract Senescent cells accumulate in most tissues with organismal aging, exposure to stressors, or disease progression. It is challenging identify senescent because cellular senescence signatures and phenotypes vary widely across distinct cell types tissues. Here we developed an analytical algorithm that defines cell-type-specific universal of a wide range We utilize 72 mouse 64 human weighted single-cell transcriptomic create the SenePy scoring platform. better recapitulate vivo than...
Abstract Human stem‐cell‐derived extracellular vesicles (EVs) are currently being investigated for cell‐free therapy in regenerative medicine applications, but the lack of noninvasive imaging methods to track EV homing and uptake injured tissues has limited refinement optimization approach. Here, we developed a new labelling strategy prepare magnetic EVs (magneto‐EVs) allowing sensitive yet specific MRI tracking systemically injected therapeutic EVs. This relies on use ‘sticky’ particles,...
Recent studies suggest that training of innate immune cells such as tissue-resident macrophages by repeated noxious stimuli can heighten host defense responses. However, it remains unclear whether trained immunity also enhances injury resolution to counterbalance the heightened inflammatory Here, we studied lung-resident alveolar (AMs) prechallenged with either bacterial endotoxin or Pseudomonas aeruginosa and observed these AMs showed greater resilience pathogen-induced cell death....
All-RNA-mediated targeted gene integration methods, rendering reduced immunogenicity, effective deliverability with non-viral vehicles, and a low risk of random mutagenesis, are urgently needed for next-generation addition technologies. Naturally occurring R2 retrotransposons hold promise in this context due to their site-specific profile. Here, we systematically analyzed the biodiversity elements screened several orthologs capable full-length insertion mammalian cells. Robust system...
The naturally occurring prokaryotic CRISPR-Cas systems provide valuable resources for the development of new genome-editing tools. However, majority Cas nucleases exhibit poor editing efficiency in mammalian cells, which significantly limits their utility. Here, we have developed a method termed Improving Editing Activity by Synergistic Engineering (MIDAS). This exerts synergistic effect to improve wide range enhancing interactions between nuclease with protospacer adjacent motif (PAM) and...
Abstract Blood–brain barrier (BBB) dysfunction is emerging as a key pathogenic factor in the progression of Alzheimer’s disease (AD), where increased microvascular endothelial permeability has been proposed to play an important role. However, molecular mechanisms leading brain AD are not fully understood. We studied female APPswe/PS1∆E9 (APP/PS1) mice which constitute transgenic mouse model amyloid-beta (Aβ) amyloidosis and found that increases with aging areas showing greatest amyloid...
Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) can model heritable arrhythmias to personalize therapies for individual patients. Although atrial fibrillation (AF) is a leading cause of cardiovascular morbidity and mortality, current platforms generate iPSC-atrial (a) CMs are inadequate modeling AF. We applied combinatorial engineering approach, which integrated multiple physiological cues, including metabolic conditioning electrical stimulation, mature iPSC-aCMs. Using...
Many signaling and other genes known as "hidden" drivers may not be genetically or epigenetically altered differentially expressed at the mRNA protein levels, but, rather, drive a phenotype such tumorigenesis via post-translational modification mechanisms. However, conventional approaches based on genomics differential expression are limited in exposing hidden drivers. Here, we present comprehensive algorithm toolkit NetBID2 (data-driven network-based Bayesian inference of drivers, version...
Abstract Traumatic brain injury (TBI)‐induced post‐traumatic epilepsy (PTE) is a serious disease that causes the loss of motor functions, cognitive impairments, and even death. Real‐time long‐term electrophysiological signal monitoring has significant implication for treatment patient with TBI. Brain–computer interfaces (BCIs) utilizing flexible electrodes enable accurate identification by electrocorticography (ECoG) monitoring. In this study, customized osteoid electrode developed, which...
With the fast pace of global urbanization, anthropogenic disturbances not only lead to frequent disasters, but also cause direct and indirect ecological economic losses. To reduce adverse effects as part sustainable ecosystem management, assessments habitat quality risk are necessary. The objectives this study analyze environmental conditions Beijing-Hangzhou Grand Canal (Suzhou section) for evaluating degradation, conduct ecological-risk early warning assessment in section. is longest first...
Abstract Topological materials carrying topological surface states (TSSs) have extraordinary carrier mobility and robustness, which provide a new platform for searching efficient hydrogen evolution reaction (HER) electrocatalysts. However, the majority of these TSSs originate from sp band quantum catalysts rather than d band. Here, based on density functional theory calculation, it is reported semimetal Pd 3 Sn mainly derived orbital proposed that optimizing state electrons by introduction...
Abstract The novel coronavirus (CoV) disease termed COVID-19 (Coronavirus Disease-19) caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) is causing a massive pandemic worldwide, threatening public health systems across the globe. During this ongoing outbreak, nucleic acid detection has played an important role in early diagnosis. Here we report protocol using CRISPR-based CRISPR diagnostic platform - CDetection (Cas12b-mediated DNA detection). By combining sample...
Studying temporal gene expression shifts during disease progression provides important insights into the biological mechanisms that distinguish adaptive and maladaptive responses. Existing tools for analysis of time course transcriptomic data are not designed to optimally identify distinct patterns when analyzing dynamic differentially expressed genes (DDEGs). Moreover, there enough methods assess visualize pathways mapped from sets. In this study, we developed an open-source R package...
The ongoing 2019 coronavirus disease pandemic (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants, is a global public health threat. Early diagnosis identification of SARS-CoV-2 variants plays critical role in COVID-19 prevention control. Currently, the most widely used technique to detect quantitative reverse transcription real-time PCR (RT-qPCR), which takes nearly 1 hour should be performed experienced personnel ensure accuracy results....
In order to calibrate the ductile fracture criterion in SUS304 stainless steel, four tensile samples were designed, their finite element models established, and uniaxial tests carried out. The simulation results compared with tests. Due limitations of software, only a few criteria can be solved MENTAT MSC.MARC 2013. make universal, HYPELA2 subroutine was used write criteria, three-point bending verify program. Finally, calibration different are test results. show that error between is small,...