A5036803806- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Extracellular vesicles in disease
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Pancreatic function and diabetes
- Zebrafish Biomedical Research Applications
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Blood groups and transfusion
- Cell death mechanisms and regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Complement system in diseases
- Renal and related cancers
- Acute Myeloid Leukemia Research
- Hippo pathway signaling and YAP/TAZ
- Neurogenesis and neuroplasticity mechanisms
- Barrier Structure and Function Studies
- Traditional Chinese Medicine Analysis
- Polysaccharides Composition and Applications
- Flavonoids in Medical Research
University of Science and Technology of China
2020-2024
Johns Hopkins University
2016-2021
Johns Hopkins Medicine
2016-2021
University of Baltimore
2019
Zhejiang University
2010-2017
Chinese University of Hong Kong
2014-2015
First Affiliated Hospital Zhejiang University
2011-2014
University of North Carolina at Charlotte
2014
Prince of Wales Hospital
2014
Chinese University of Hong Kong, Shenzhen
2014
The newly developed transcription activator-like effector protein (TALE) and clustered regularly interspaced short palindromic repeats/Cas9 factors (TF) offered a powerful precise approach for modulating gene expression. In this article, we systematically investigated the potential of these new tools in activating stringently silenced pluripotency Oct4 (Pou5f1) mouse human somatic cells. First, with number TALEs sgRNAs targeting various regions promoters, found that most efficient TALE-VP64s...
Extracellular vesicles (EVs), including exosomes and microvesicles, mediate intercellular communications exert various biological activities via delivering unique cargos of functional molecules such as RNAs proteins to recipient cells. Previous studies showed that EVs produced secreted by human mesenchymal stem cells (MSCs) can substitute intact MSCs for tissue repair regeneration. In this study, we examined properties functions from induced pluripotent (iPSCs) be cultured infinitely under a...
Abstract Human stem‐cell‐derived extracellular vesicles (EVs) are currently being investigated for cell‐free therapy in regenerative medicine applications, but the lack of noninvasive imaging methods to track EV homing and uptake injured tissues has limited refinement optimization approach. Here, we developed a new labelling strategy prepare magnetic EVs (magneto‐EVs) allowing sensitive yet specific MRI tracking systemically injected therapeutic EVs. This relies on use ‘sticky’ particles,...
Abstract Developing an intracellular delivery system is of key importance in the expansion protein-based therapeutics acting on cytosolic or nuclear targets. Recently, extracellular vesicles (EVs) have been exploited as next-generation modalities due to their natural role intercellular communication and biocompatibility. However, fusion protein interest a scaffold represents widely used strategy for cargo enrichment EVs, which could compromise stability functionality cargo. Herein, we report...
Abstract Glioblastomas (GBM) are highly infiltrated by myeloid-derived innate immune cells that contribute to the immunosuppressive nature of brain tumor microenvironment (TME). CD47 has been shown mediate evasion, as CD47–SIRPα axis prevents phagocytosis macrophages and other myeloid cells. In this study, we established homozygous deletion (CD47−/−) in human mouse GBM investigated impact eliminating "don't eat me" signal on growth tumor–TME interactions. knockout (KO) did not significantly...
Abstract The generation of cultured red blood cells (cRBCs) ex vivo represents a potentially unlimited source for RBC transfusion and other cell therapies. Human cRBCs can be generated from the terminal differentiation proliferating erythroblasts derived hematopoietic stem/progenitor or erythroid precursors in peripheral mononuclear cells. Efficient maturation into highly depend on replenishing human plasma, which exhibits variable potency across donors batches complicates consistent cRBC...
Bone marrow mesenchymal stem cells (MSCs) are promising candidates for the treatment of various inflammatory disorders due to their profound immunomodulatory properties. However, immunosuppressive capacity MSCs needs activation by an microenvironment, which may negatively impact therapeutic effect because increased immunogenicity. Here we explore role mammalian target rapamycin (mTOR) signaling on MSCs, and its immunogenicity in microenvironment. Human bone were cocultured with activated...
Compromised regeneration resulting from the deactivation of Wnt/β-catenin signaling contributes to progression chronic obstructive pulmonary disease (COPD) with limited therapeutic options. Extracellular cytokine-induced Wnt-based provides an alternative option for COPD treatment. However, hydrophobic nature Wnt proteins limits their purification and use. This study devises a strategy deliver membrane-bound wingless-type MMTV integration site family, member 3A (Wnt3a) over long distance by...
The efficient commitment of a specialized cell type from induced pluripotent stem cells (iPSCs) without contamination unknown substances is crucial to their use in clinical applications. Here, we propose that CD34+ progenitor cells, which retain hematopoietic and endothelial potential, could be efficiently obtained iPSCs derived human bone marrow mesenchymal (hBMMSC-iPSCs) with defined factors. By treatment cocktail containing mesodermal, hematopoietic, inducers (BMP4, SCF, VEGF,...
Transfusion of red blood cells (RBCs) from ABO-matched but genetically unrelated donors is commonly used for treating anemia and acute loss. Increasing demand insufficient supply donor RBCs, especially those universal types or free known unknown pathogens, has called ex vivo generation functional RBCs by large-scale cell culture. However, generating physiological numbers transfusable cultured (cRBCs) remains challenging, due to our inability either extensively expand primary RBC precursors...
The efficient generation of hematopoietic stem cells (HSCs) from human-induced pluripotent (iPSCs) holds great promise in personalized transplantation therapies. However, the derivation functional and transplantable HSCs iPSCs has had very limited success thus far. We developed a synthetic 3D niche system comprising nanofibers seeded with bone marrow (BM)-derived stromal growth factors to induce human vitro. Approximately 70 % CD34+ accompanied CD43+ progenitor could be derived this...
Precise genome editing in human induced pluripotent stem cells (iPSCs) significantly enhances our capability to use iPSCs for disease modeling, drug testing and screening as well investigation of cell biology. In this study, we seek achieve conditional expression the CD55 gene order interrogate its functions. We used two iPSC lines that have unique genotypes, constructed an inducible Cas9 system is integrated at AAVS1 safe harbor site genome. Using paired guide RNAs, observed efficient...
Transfusion of red blood cells (RBCs) is a life-saving intervention for anemic patients. Human induced pluripotent stem (iPSC) have the capability to expand and differentiate into RBCs (iPSC-RBCs). Here we developed murine model investigate in vivo properties human iPSC-RBCs. iPSC lines were produced from peripheral mononuclear by transient expression plasmids containing OCT4, SOX2, MYC, KLF4, BCL-XL genes. iPSC-RBCs generated culture supplemented with platelet lysate, CD34- CD235a+ CD233+...
Alzheimer's disease (AD) is a progressive neurodegenerative that the major cause of dementia in older people. Here, we report derivation human induced pluripotent stem cells (iPSCs) from an AD patient at age 80 who has APOE ε4/ε4 genotype and resilient to cognitive decline for 10 years. The iPSCs reprogrammed blood this by transient expression pluripotency genes maintain genotype, are karyotypically normal display typical iPSC characteristics. Upon differentiation, able differentiate into...
Activating point mutations in the MPL gene encoding thrombopoietin receptor are found 3%-10% of essential thrombocythemia (ET) and myelofibrosis patients. Here, we report derivation induced pluripotent stem cells (iPSCs) from an ET patient with a heterozygous V501L mutation. Peripheral blood CD34+ progenitor were reprogrammed by transient plasmid expression OCT4, SOX2, KLF4, c-MYC plus BCL2L1 (BCL-xL) genes. The derived line M494 carries mutation, displays typical iPSC morphology...
The efficient commitment of a specialized cell type from induced pluripotent stem cells (iPSCs) without contamination unknown substances is crucial to their use in clinical applications.Here, we propose that CD34+ progenitor cells, which retain hematopoietic and endothelial potential, could be efficiently obtained iPSCs derived human bone marrow mesenchymal (hBMMSC-iPSCs) with defined factors.By treatment cocktail containing mesodermal, hematopoietic, inducers (BMP4, SCF, VEGF, respectively)...