A5075256727- Liver physiology and pathology
- Pancreatic function and diabetes
- Organ Transplantation Techniques and Outcomes
- Pluripotent Stem Cells Research
- Xenotransplantation and immune response
- Tissue Engineering and Regenerative Medicine
- Galectins and Cancer Biology
- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Diabetes and associated disorders
- Organ and Tissue Transplantation Research
- Diabetes Management and Research
- CRISPR and Genetic Engineering
- Pediatric Hepatobiliary Diseases and Treatments
- Mesenchymal stem cell research
- Phagocytosis and Immune Regulation
- Toxin Mechanisms and Immunotoxins
- Genetics and Neurodevelopmental Disorders
- Immune Cell Function and Interaction
- 3D Printing in Biomedical Research
- COVID-19 Clinical Research Studies
- Gallbladder and Bile Duct Disorders
- Pancreatitis Pathology and Treatment
- Glycosylation and Glycoproteins Research
- Transplantation: Methods and Outcomes
Universidad Panamericana
2019-2024
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
2018-2024
University of Colorado Anschutz Medical Campus
2020-2024
University of Colorado Denver
2020-2024
Children's Hospital Colorado
2022
Columbia University Irving Medical Center
2011-2021
Harvard University
2010-2020
Massachusetts General Hospital
2010-2020
Creative Commons
2020
National Institute of Allergy and Infectious Diseases
2016
Excessive systemic inflammation following trauma, sepsis, or burn could lead to distant organ damage. The transplantation of bone marrow stromal cells mesenchymal stem (MSCs) has been reported be an effective treatment for several immune disorders by modulating the inflammatory response injury. We hypothesized that MSCs can dynamically secrete factors neutralize activity cytokines. In this study, we showed cocultured are able decrease nuclear factor κ-B (NFκB) activation in target epithelial...
Since the first attempt of pig-to-primate liver xenotransplantation (LXT) in 1968, survival has been limited. We evaluated a model utilizing α-1,3-galactosyltransferase knockout donors, continuous posttransplant infusion human prothrombin concentrate complex, and immunosuppression including anti-thymocyte globulin, FK-506, methylprednisone, costimulation blockade (belatacept, n = 3 or anti-CD40 mAb, 1) to extend survival. Baboon 1 remained well until postoperative day (POD) 25, when...
Abstract Severe COVID-19 is associated with a systemic hyperinflammatory response leading to acute respiratory distress syndrome (ARDS), multi-organ failure, and death. Galectin-3 ß-galactoside binding lectin known drive neutrophil infiltration the release of pro-inflammatory cytokines contributing airway inflammation. Thus, we aimed investigate potential galectin-3 as biomarker severe outcomes. We prospectively included 156 patients RT-PCR confirmed COVID-19. A outcome was defined...
Artificial bones have often used for bone regeneration due to their strength, but they cannot provide an adequate environment cell penetration and settlement. We therefore attempted explore various materials that may allow the cells penetrate engraft in defects. PuraMatrix is a self-assembling peptide scaffold produces nanoscale allowing both cellular engraftment. The objective of this study was investigate effect on mouse defect model calvaria. Matrigel as control. expression bone-related...
Cirrhosis is considered a growing cause of morbidity and mortality, which represents significant public health problem. Currently, there no effective treatment to reverse cirrhosis. Treatment primarily centers on addressing the underlying liver condition, monitoring, managing portal hypertension-related complications, evaluating potential for transplantation in cases decompensated cirrhosis, marked by rapid progression emergence complications like variceal bleeding, hepatic encephalopathy,...
Human embryonic stem (hES) cells have recently been studied as an attractive source for the development of a bioartificial liver (BAL). Here we evaluate differentiation capacity hES into hepatocytes. were subjected to suspension culture 5 days, and then cultured onto poly-amino-urethane (PAU)-coated, nonwoven polytetrafluoroethylene (PTFE) fabric in presence fibroblast growth factor-2 (bFGF) (100 ng/ml) 3 with deleted variant hepatocyte factor (dHGF) 1% dimethyl sulfoxide (DMSO) 8 finally...
Islet transplantation is associated with an elevated rate of early graft failure. The isolation process leads to structural and functional abnormalities. reestablishment the cell–matrix relationship important modulate survival function islets. Thus, we evaluated effect human fibronectin (hFN) self-assembling peptide nanofiber (SAPNF) in ability support islet vitro after into streptozotocin (STZ)-induced diabetic severe combined immunodeficiency (SCID) mice. Human isolated islets were...
Cell-based technologies to support/restore liver function represent one of the most promising opportunities in treatment acute failure. However, understanding constituent cell types that interact achieve liver-specific structure and has not been achieved development assist devices (LADs). Here we show hepatocytes migrated toward adhered formed sinusoids-like structures conjunction with nonparenchymal cells, this organoid sophisticated tissue after 7 days an implanted LAD rodents. Hepatocytes...
Abstract Background Pig to baboon liver xenotransplantation typically results in severe thrombocytopenia and coagulation disturbances, culminating death from hemorrhage within 9 days, spite of continuous transfusions. We studied the contribution anticoagulant production clotting pathway deficiencies fatal bleeding recipients porcine livers. Methods By transplanting xenografts α1,3‐galactosyltransferase gene‐knockout (GalT‐ KO ) miniature swine donors into baboons as auxiliary organs, leaving...
We sought to determine the effects of exogenous administration human coagulation factors following pig-to-baboon liver xenotransplantation (LXT) using GalT-KO swine donors. After LXT, baboons received no (historical control, n=1), bolus a prothrombin concentrate complex (hPCC; 2.5 mL/kg, n=2), continuous infusion hPCC (1.0 mL/h, n=1) or recombinant factor VIIa (1 µg/kg per hour, n=3). The historical control recipient demonstrated persistent thrombocytopenia despite platelet after transplant,...
Chronic non-progressive pneumonia, a disease that has become worldwide epidemic caused considerable loss to sheep industry. Mycoplasma ovipneumoniae (M. ovipneumoniae) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold immunoblotting elongation factor Tu (EF-Tu) heat shock protein 70 (HSP 70) are membrane-associated proteins on M. ovipneumonaiea. evaluated humoral cellular immune responses vivo immunizing BALB/c mice with both...
Abstract Background & Aims Patients with advanced cirrhosis often have immune dysfunction and are more susceptible to infections. Galectin‐3 is a β‐galactoside‐binding lectin implicated in inflammation, regulation liver fibrosis. We aim investigate galectin‐3 expression its ability predict post‐transplant infectious complications. Methods collected sera samples from 129 cirrhotic patients at the time of transplantation an external cohort 37 alcoholic disease including hepatitis (AH)...
Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide source differentiated for many therapeutic uses. Here we investigated an efficient method hepatic differentiation from hES cells. A human ES line, KhES-1, was used maintained by nonfeeder method. KhES-1 were cultured 5 days in presence activin (50 ng/ml) then treated with deleted variant hepatocyte growth factor (dHGF) at 0, 100, or 500 ng/ml 7 days. The...