A5084153000- Pancreatic and Hepatic Oncology Research
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Plant Gene Expression Analysis
- Phagocytosis and Immune Regulation
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Acute Lymphoblastic Leukemia research
- Lung Cancer Treatments and Mutations
- Axon Guidance and Neuronal Signaling
- Acute Myeloid Leukemia Research
- Wnt/β-catenin signaling in development and cancer
- Acute Myocardial Infarction Research
- Histone Deacetylase Inhibitors Research
- Neuroendocrine Tumor Research Advances
- Peptidase Inhibition and Analysis
- Cancer and Skin Lesions
- Hedgehog Signaling Pathway Studies
- Angiogenesis and VEGF in Cancer
- Sepsis Diagnosis and Treatment
- Cardiac Imaging and Diagnostics
University of Verona
2018-2024
Vita-Salute San Raffaele University
2023-2024
Associazione Nazionale Medici Cardiologi Ospedalieri
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2023
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2023
University Hospital Foundation
2019-2021
Fondazione Lanza
2008-2021
Ospedali Riuniti Umberto I
2020
Azienda Ospedaliera Universitaria Integrata Verona
2020
Santa Maria Nuova Hospital
2019
Mesenchymal stromal cells (MSCs) are adult, multipotent of mesodermal origin representing the progenitors all tissues. MSCs possess significant and broad immunomodulatory functions affecting both adaptive innate immune responses once primed by inflammatory microenvironment. Recently, role extracellular vesicles (EVs) in mediating therapeutic effects has been recognized. Nevertheless, molecular mechanisms responsible for properties MSC-derived EVs (MSC-EVs) still poorly characterized....
Background Complex tumor and immune microenvironment render pancreatic ductal adenocarcinoma (PDAC) resistant to checkpoint inhibitors (ICIs). Therefore, a strategy convert the hostile into an immunopermissive is required. Recent studies showed that intratumoral injection of Toll-like receptor 9 agonist IMO-2125 primes adaptive response. Phase I II trials with demonstrated its safety antitumoral activity. Methods We generated array preclinical models by orthotopically engrafting PDAC-derived...
The identification of pancreatic ductal adenocarcinoma (PDAC) dysregulated genes may unveil novel molecular targets entering inhibitory strategies. Laminins are emerging as potential in PDAC given their role diagnostic and prognostic markers. Here, we investigated the cellular, functional, clinical relevance LAMC2 its regulated network, with ultimate goal identifying therapies.LAMC2 expression was analyzed tissues, a panel human mouse cell lines, genetically engineered model. Genetic...
Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are rare and highly heterogeneous neoplasms whose incidence has markedly increased over the last decades. A grading system based on tumor cells’ proliferation index predicts high-risk for G3 NETs. However, low-to-intermediate grade (G1/G2) NETs have an unpredictable clinical course that varies from indolent to malignant. Cultures of human cancer cells enable perform functional perturbation analyses instrumental enhance our understanding...
The dysregulation of the axon guidance pathway is common in pancreatic ductal adenocarcinoma (PDAC), yet our understanding its biological relevance limited. Here, we investigated functional role cue SEMA3A supporting PDAC progression.
Abstract Solid pseudopapillary neoplasms (SPN) of the pancreas are rare, low‐grade malignant that metastasise to liver or peritoneum in 10–15% cases. They almost invariably present somatic activating mutations CTNNB1 . No comprehensive molecular characterisation metastatic disease has been conducted date. We performed whole‐exome sequencing and copy‐number variation (CNV) analysis 10 primary SPN comparative five matched primary/metastatic tumour specimens by high‐coverage targeted 409 genes....
Abstract Notch3 and Notch4 support survival of primary B-cell acute lymphoblastic leukemia (B-ALL) cells, suggesting a role for Notch signaling in drug response. Here we used vitro, silico, vivo mouse xenograft model-based approaches to define the pathway B-ALL chemosensitivity. We observed significant receptor ligand expression cells cell lines. Primary from high-risk patients overexpressed Notch3, Notch4, Jagged2 while displaying reduction levels Notch1-4 following chemotherapy. then...
In this study, we compared the overall gene and pathway expression profiles of HS-5 HS-27A stromal cell lines with those primary bone marrow MSCs to verify if they can be considered a reliable alternative tool for evaluating contribution in tumor development immunomodulation. Indeed, due their easier manipulation vitro as MSC cultures, several published studies took advantage assess biological mechanisms mediated by cells influencing biology immune responses. However, process carried out...
High temperature during grape berry ripening impairs the quality of fruits and wines. Veraison time, which marks onset, is a key factor for determining climatic conditions ripening. Understanding its genetic control crucial to successfully breed varieties more adapted changing climate. Quantitative trait loci (QTL) studies attempting elucidate determinism developmental stages in grapevine have identified wide genomic regions. Broad scale transcriptomic studies, by identifying sets genes...
A number of genes have been implicated in the pathogenesis BCC addition to Hedgehog pathway, which is known drive initiation this tumour. We performed in-depth analysis 13 BCC-related (CSMD1, CSMD2, DPH3 promoter, PTCH1, SMO, GLI1, NOTCH1, NOTCH2, TP53, ITIH2, DPP10, STEAP4, TERT promoter) 57 lesions (26 superficial and 31 nodular) from 55 patients their corresponding blood samples. PTCH1 TP53 mutations were found 71.9% 45.6% BCCs, respectively. high mutation rate was also detected CSMD1...
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with limited therapeutic options. However, metabolic adaptation to the harsh PDAC environment can expose liabilities useful for therapy. Targeting key regulator mechanistic target of rapamycin complex 1 (mTORC1) and its downstream pathway shows efficacy only in subsets patients but gene modifiers maximising response remain be identified.Three independent cohorts were studied correlate PI3K-C2γ protein abundance outcome....
Pancreatic ductal adenocarcinoma (PDA) has a highly immunosuppressive microenvironment, which is contributed by the complex interaction between cancer cells and heterogeneous population of stromal cells. Therefore, facile trackable models are needed for integrative dynamic interrogation cancer-stroma interaction. Here, we tracked immunoevolution PDA in genetically-defined transplantable model mouse pancreatic tumour organoids that recapitulates progression disease from early preinvasive...
Worsening of renal function (WRF) in acute heart failure (AHF) strongly predicts adverse clinical outcome. Plasma neutrophil gelatinase-associated lipocalin (NGAL) has been proposed as an earlier biomarker tubular damage, but important methodological issues remain unsolved, particularly AHF.In 30 consecutive patients admitted for AHF, 108 serum NGAL (Alere system) measurements were performed at entry and the first days recovery, reproducibility within same blood samples was very high (r =...
The role of Notch signaling in acute myeloid leukemia (AML) is still under investigation. We have previously shown that high levels receptors and ligands could interfere with drug response. In this study, the protein expression 79 AML blast samples collected from newly diagnosed patients was examined through flow cytometry. Gamma-secretase inhibitors were used mouse xenograft models to evaluate contribution pharmacological inhibition survival. univariate analysis for testing correlation...
Wnt/β-catenin signaling has been reported in Acute Myeloid leukemia, but little is known about its significance as a prognostic biomarker and drug target. In this study, we first evaluated the correlation between expression levels of Wnt molecules clinical outcome. Then, studied—in vitro vivo—the anti-leukemic value combinatorial treatment inhibitors classic anti-leukemia drugs. Higher β-catenin, Ser675-phospho-β-catenin GSK-3α (total Ser 9) were found AML cells from intermediate or poor...
Ruxolitinib is an anti-inflammatory drug that inhibits the Janus kinase-signal transducer (JAK-STAT) pathway on surface of immune cells. The potential targeting this using JAK inhibitors a promising approach in patients affected by coronavirus disease 2019 (COVID-19). was provided as compassionate use consecutively admitted to our institution for severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection. Inclusion criteria were oxygen saturation less than or equal 92%, signs...
Abstract Transcriptomic analyses of pancreatic ductal adenocarcinoma (PDAC) have identified two major epithelial subtypes with distinct biology and clinical behaviours. Here, we aimed to clarify the role FGFR1 FGFR4 in definition aggressive PDAC phenotypes. We found that expression is exclusively detected cells, significantly elevated classical subtype, associates better outcomes. In highly basal-like/squamous PDAC, reduced aligns hypermethylation gene lower levels histone marks associated...
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related mortality among adults in developed countries. The discovery most common genetic alterations as well development organoid models pancreatic cancer have provided insight into fundamental pathways driving tumor progression from a normal cell to non-invasive precursor lesion and finally widely metastatic disease, offering new opportunities for identifying key driver evolution. Obesity one serious public health...
Fibroblast heterogeneity is increasingly recognised across cancer conditions. Given their important contribution to disease progression, mapping fibroblasts' critical devise effective anti-cancer therapies. Cancer-associated fibroblasts (CAFs) represent the most abundant cell population in pancreatic ductal adenocarcinoma (PDAC). Whether CAF phenotypes are differently specified by PDAC lineages remains be elucidated. Here, we reveal an role for MAPK signalling pathway defining phenotypes. We...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with few available therapeutic options. Two transcriptional cancer cell states have been consistently reported in PDAC, the basal-like/squamous phenotype displaying more aggressive biological behavior. Genetic and epigenetic dysregulation of axon guidance pathway are common yet our understanding its relevance limited. Here, we investigated functional role cue SEMA3A sustaining progression PDAC. We integrated transcriptomic...