A5072426551- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Antibiotic Resistance in Bacteria
- Alzheimer's disease research and treatments
- Chromosomal and Genetic Variations
- Primate Behavior and Ecology
- Evolution and Genetic Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Neuroscience and Neuropharmacology Research
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Zebrafish Biomedical Research Applications
- Cholinesterase and Neurodegenerative Diseases
- Retinal Development and Disorders
- Bat Biology and Ecology Studies
- Probiotics and Fermented Foods
- Animal Behavior and Reproduction
- Genomics, phytochemicals, and oxidative stress
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Polyomavirus and related diseases
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
Central Institute for Experimental Animals
2015-2024
RIKEN Center for Brain Science
2020-2022
Hitachi (Japan)
2022
National Institute of Health Sciences
2011-2015
Tokyo Medical and Dental University
2007-2010
Kōchi University
2005
Abstract Genetically modified nonhuman primates (NHP) are useful models for biomedical research. Gene editing technologies have enabled production of target-gene knock-out (KO) NHP models. Target-gene-KO/knock-in (KI) efficiency CRISPR/Cas9 has not been extensively investigated in marmosets. In this study, optimum conditions target gene modification efficacies CRISPR/mRNA and CRISPR/nuclease marmoset embryos were examined. was more effective than avoiding mosaic genetic alteration....
Summary Alzheimer’s disease (AD) is a major cause of dementia, with the number patients this condition anticipated to exceed 50 million worldwide in near future. Despite extensive research efforts, no effective measures are available facilitate prevention or treatment AD, which due part lack animal models able closely replicate human-like state. Here, we describe generation three mutant marmoset individuals exon 9 PSEN1 gene product has been deleted ( -ΔE9). Such ΔE9 mutations have reported...
Assessment of social interactions and behavioral changes in nonhuman primates is useful for understanding brain function during life events pathogenesis neurological diseases. The common marmoset (Callithrix jacchus), which lives a nuclear family like humans, model, but longitudinal automated observation multiple animals has not been achieved. Here, we developed Full Monitoring Animal Identification (FulMAI) system detection three-dimensional (3D) trajectories each individual marmosets under...
Abstract The first draft of the common marmoset ( Callithrix jacchus ) genome was published by Marmoset Genome Sequencing and Analysis Consortium. based on whole-genome shotgun sequencing current assembly version is Callithrix_jacches-3.2.1, but there still exist 187,214 undetermined gap regions supercontigs relatively short contigs that are unmapped to chromosomes in genome. We performed resequencing deep with high-throughput technology. Several different sequence runs using Illumina...
Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1), a latent viral protein consistently expressed in infected proliferating cells, is essentially required trans to maintain EBV episomes cells. We constructed mutant (mt) EBNA1 and examined whether it exerted dominant-negative effects on maintenance of the episome thereby leading abrogation EBV-infected tumor cell growth. Using lymphocyte epithelial lines converted with neomycin-resistant recombinant (rEBV) as models, adenovirus...
Transgenic gpt delta mouse and rat models were developed to perform Spi(-) assays for in vivo mutagenicity tests. The animals established by integration of lambda EG10 phage DNA as a transgene into the genome. inserted position on chromosome was determined fluorescent situ hybridization Southern blot analyses; however, exact sequence junction not known. To identify site pattern genomic copies, DNAs extracted from C57BL/6J mice F344 rats applied whole genome sequencing mate-pair analysis.The...
Nonhuman primates (NHPs), which are closely related to humans, useful in biomedical research, and an increasing number of NHP disease models have been reported using gene editing. However, many disease-related genes cause perinatal death when manipulated homozygously by In addition, resources, limited, should be efficiently used. Here, address these issues, we developed a method introducing heterozygous genetic modifications into common marmosets combining Platinum transcription...
Abstract Background The common marmoset ( Callithrix jacchus ) is one of the most studied primate model organisms. However, genomes available in public databases are highly fragmented and filled with sequence gaps, hindering research advances related to genomics transcriptomics. Results Here we utilize single-molecule, long-read data improve update existing genome assembly report a near-complete marmoset. 2.79 Gb size, contig N50 length 6.37 Mb chromosomal scaffold 143.91 Mb, representing...
Abstract Background Alzheimer’s disease (AD) is the leading cause of dementia worldwide. Despite considerable research efforts, exact pathomechanisms still remain unknown, and no effective treatment available. AD field have mainly utilized mouse models for decades, but species differences between rodents primates may constrain us from understanding precise mechanisms. Method We Transcription Activator‐Like Effector Nuclease (TALEN) to delete 3’ splicing site (SS) exon 9 presenilin 1‐encoding...
Abstract Accurate assessment of behavioral changes and social interactions in mammalian models is necessary to elucidate the pathogenesis neurological disorders. The common marmoset ( Callithrix jacchus ) a useful model this regard. However, measurements, including assessments sociality free-moving group animals, have not been conducted marmosets. Here, we developed new analysis system for three-dimensional (3D) trajectories independently multiple individuals by combining video tracking, 3D...
Abstract Aging is a primary risk factor of Alzheimer’s disease (AD), with the world-wide number patients anticipated shortly to exceed 50 million. Despite extensive research efforts, no effective measures are available for its prevention or treatment due in part lack human-like animal models. Here, we describe generation three mutant marmoset individuals which exon 9 PSEN1 gene product deleted (PSEN1-ΔE9); ΔE9 mutations have been reported cause early onset familial AD1-5. We used...