A5063108517- Gut microbiota and health
- Diet and metabolism studies
- Microbial infections and disease research
- Virus-based gene therapy research
- Clostridium difficile and Clostridium perfringens research
- Rabies epidemiology and control
- Viral gastroenteritis research and epidemiology
- Probiotics and Fermented Foods
- Antifungal resistance and susceptibility
- Animal testing and alternatives
- Enterobacteriaceae and Cronobacter Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Pharmacological Effects of Natural Compounds
- Metabolomics and Mass Spectrometry Studies
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Neuroendocrine regulation and behavior
- Animal Virus Infections Studies
- Bartonella species infections research
- Hepatitis B Virus Studies
- RNA Interference and Gene Delivery
- Viral Infections and Vectors
- Immune Cell Function and Interaction
- Dietary Effects on Health
Central Institute for Experimental Animals
2000-2023
Intestinal microbiota and their metabolites are strongly associated with host physiology. Developments in DNA sequencing mass spectrometry technologies have allowed us to obtain additional data that enhance our understanding of the interactions among microbiota, metabolites, host. However, strategies used analyze these datasets not yet well developed. Here, we describe an original analytical strategy, metabologenomics, consisting integrated analysis spectrometry-based metabolome...
Commensal microbiota colonize the surface of our bodies. The inside gastrointestinal tract is one such that provides a habitat for them. long organ system comprising various parts, and each part possesses functions. It has been reported composition intestinal luminal metabolites between small large intestine are different; however, comprehensive metabolomic commensal profiles specific to lumen remain obscure. In this study, by using capillary electrophoresis time-of-flight mass spectrometry...
ABSTRACT The prophylactic effect of FK463, a new water-soluble echinocandin-like lipopeptide with inhibitory activity against 1,3-β- d -glucan synthase, Pneumocystis carinii infection was investigated the severe combined immunodeficient (SCID) mouse model. Treatment pentamidine, and saline only performed for 6 weeks from day after SCID mice were inoculated intranasally infected lung homogenates. FK463 at 0.2 or 1.0 mg/kg body weight, pentamidine 4 mg/kg, subcutaneously administered daily...
Humanized mice are widely used to study the human immune system in vivo and investigate therapeutic targets for various diseases. Immunodeficient NOD/Shi-scid-IL2rγnull (NOG) transferred with hematopoietic stem cells a useful model studying systems analyzing engrafted cells. The gut microbiota plays significant role development function of maintenance homeostasis; however, there is currently no available animal that has been reconstituted vivo. In this study, we established new CD34+...
In this study, hypochlorous acid solution, a weak acid, provided as drinking water to rats, was evaluated for its ability eradicate and prevent Pseudomonas aeruginosa infection, while monitoring simultaneous effect on serum biochemical variables microbiota in the rat cecum. The results suggest that solution could not eliminate bacteria experimentally infected rats; however, administration of 10-parts-per-million (ppm) effective inhibiting horizontal spread P. infection among cage mates....
The fecal microbiota of six mice derived from three Japanese commercial breeders was analyzed by using 16S rRNA gene clone libraries to construct a database for analyzing the gut laboratory mice. 566 clones were obtained generated DNA samples BALB/c, C57BL/6N, DBA/2 and ICR Among these clones, there 446 unique sequences. When grouped at 98% similarity level, sequences consisted 103 Clostridiales, 43 Bacteroidales, 5 Lactobacillus 3 Erysipelotricaceae, as well 11 other phyla.
Cilia-associated respiratory (CAR) bacillus was detected by means of the reverse transcription (RT)-polymerase chain reaction (PCR), and results were compared with those indirect immunofluorescence test (IFAT) for detection organism. In experimental infections, 15 mice in contact previously inoculated CAR bacillus. Three each tested at days 3, 5, 7, 12 20 postexposure. On day 3 postexposure, oral swab samples from all RT-PCR, but not any sampling sites IFAT. Total numbers positive nasal,...
Enrofloxacin, a fluoroquinolone bactericidal antibiotic, was administered in an attempt to eradicate Pasteurella pneumotropica (P. pneumotropica) from contaminated mouse colony. Contaminated mice, maintained within 4 animal rooms, were Enrofloxacin drinking water at daily dosage of 25.5 mg/kg for 2 weeks. Following one week treatment, mice selected randomly each room and examined P. pneumotropica. This procedure repeated two or three times until all tested negative the strain. With exception...
A polymerase chain reaction with new primers (new PCR) designed from Pasteurella pneumotropica 16S rDNA as an identification system for this organism was compared the PCR reported by Wang et al. (Wang's using 15 bacterial reference species and 70 clinical isolates conventional system. For strains, both PCRs were identical. isolates, Wang's showed consistency in 62.9% (44/70) 51.4% (36/70), respectively. Twenty-six inconsistent respect to morphology serology. These findings suggested that...
Abstract Recent studies using germ-free mice have demonstrated that microbiota functional roles in host homeostasis1,2. However, the phylogenetic distance between rodents and humans translates into differences their metabolism, immune response, neural function colonisation abilities. Hence, translational research nonhuman primates (NHPs) is important for bridging gap rodent human medicine3. Although several attempts to produce NHPs were made more than 50 years ago4,5, currently none are...
Murine pathogenic Escherichia coli O115a,c:K(B) (MPEC) is the causative agent of mouse megaenteron, pathology which resembles that transmissible murine colonic hyperplasia caused by Citrobacter rodentium. We compared their genetic and pathological features to reveal relationship between these two bacteria. To evaluate distances, 16S rDNA genes were sequenced biochemical reactions tested. Mouse strain susceptibility tests, using CF1 MPEC-susceptible germfree mice BALB/cA(Jic) resistant...
To avoid microbial contamination risk, vinyl film isolators are generally used in animal microbiome experiments involving germ-free (GF) mice and/or gnotobiotic (GB) mice. However, it can take several months to gain expertise operating the isolator competently. Furthermore, sterilization and sterility testing, which essential for preparation, more than 20 days. Hence, we built an experimental rearing environment that combines individual ventilation cage system a bioBUBBLE clean room...
Restriction endonuclease analysis of amplified nucleocapsid protein genes from mouse hepatitis virus (MHV) was used to differentiate 12 strains isolated liver or transplantable tumors five facilities, and the restriction patterns isolates were compared with those well-defined MHV strains, A59, JHM, 2, S Nu-67. The 10 three facilities same as that remaining two revealed different reference strains. This study showed reverse transcription polymerase chain reaction assay based are feasible for...
As basic probiotics studies, the glucose tolerance test (GTT), insulin (ITT), and adipokine hepatic enzyme activities were investigated in male C57BL/6JJcl (B6J) mice under germfree (GF) or specific pathogen free (SPF) conditions. GF B6J reproduced by reproductive engineering cesarean section using a vinyl isolators (GF group). Some group transferred to other SPF conditions (SPF In addition, conventional bred an open room defined as controls (Conv GTT, ITT, sampling of blood, liver, white...
Abstract Background: Aging is a progressive decline of cellular functions that ultimately affects whole-body homeostasis. Alterations in the gut microbiota associated with aging have been reported, however, these studies were mainly based on simple comparison young and old subjects. In this study, we longitudinally analyzed fecal microbial community composition, together plasma metabolomes, C57BL/6J mice over period 72 weeks. Result: By using longitudinal microbiome metabolome analyses,...