A5051917166- Antibiotic Resistance in Bacteria
- Fungal Infections and Studies
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- Antifungal resistance and susceptibility
- COVID-19 Clinical Research Studies
- Antimicrobial Resistance in Staphylococcus
- Bacillus and Francisella bacterial research
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Immune Response and Inflammation
- Nosocomial Infections in ICU
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- SARS-CoV-2 and COVID-19 Research
- Infective Endocarditis Diagnosis and Management
- Enterobacteriaceae and Cronobacter Research
- Bacteriophages and microbial interactions
- Long-Term Effects of COVID-19
- Central Venous Catheters and Hemodialysis
- Actinomycetales infections and treatment
- Infectious Diseases and Mycology
- Infectious Disease Case Reports and Treatments
- Plant Pathogens and Fungal Diseases
University of Tokyo Hospital
2016-2025
The University of Tokyo
2012-2022
National Institute of Infectious Diseases
2006-2019
Infection Prevention Society
2013
National Institute of Allergy and Infectious Diseases
2010-2011
National Institutes of Health
2010-2011
Office of Infectious Diseases
2003
Abstract We investigated the expression of a panel Toll-like receptors (TLRs) and their functions in human eosinophils. Eosinophils constitutively expressed TLR1, TLR4, TLR7, TLR9, TLR10 mRNAs (TLR4 greater than TLR6). In contrast, neutrophils larger variety TLR (TLR1, TLR2, TLR6, TLR8 TLR5, TLR7). Although levels eosinophils were generally less prominent compared with those neutrophils, higher level TLR7. Furthermore, among various ligands...
Bacillus anthracis infects hosts as a spore, germinates, and disseminates in its vegetative form. Production of anthrax lethal edema toxins following bacterial outgrowth results host death. Macrophages inbred mouse strains are either sensitive or resistant to toxin depending on whether they express the responsive non-responsive alleles inflammasome sensor Nlrp1b (Nlrp1bS/S Nlrp1bR/R, respectively). In this study, was shown affect susceptibility infection. Inbred congenic mice harboring...
The mechanisms by which lipopolysaccharide (LPS) is recognized, and how such recognition leads to innate immune responses, are poorly understood. Stimulation with LPS induces the activation of a variety proteins, including mitogen-activated protein kinases (MAPKs) NF-κB. Activation tyrosine (PTKs) also necessary for number biological responses LPS. We used murine macrophage-like cell line, RAW264.7, demonstrate that Janus kinase (JAK)2 phosphorylated immediately after stimulation....
Bacillus cereus is one of the pathogens causing nosocomial bloodstream infections (BSIs). However, few reports have documented antimicrobial susceptibility and clinical characteristics BSI importance empirical therapy. The aim this study was to investigate B. isolates from patients with analyze impact appropriate therapy on outcome BSI.All adult cases bacteremia between April 2003 March 2012 in a teaching hospital Tokyo, Japan were reviewed retrospectively. Clinical data collected patients'...
The molecular mechanisms by which pathogen-associated patterns recognized TLR2, such as peptidoglycan (PGN), induce homotolerance are largely unknown. It was recently reported that IRAK-M negatively regulates TLR signaling. In this study, we elucidate the of tolerance induced PGN, with a focus on role IRAK-M. We demonstrate pretreatment macrophage RAW264.7 cells high concentration (30 μg/ml) PGN for 16 h effectively induces against following stimulation 30 μg/ml PGN; while low (1 does not....
Abstract Background A heterogeneous clinical phenotype is a characteristic of coronavirus disease 2019 (COVID‐19). Therefore, investigating biomarkers associated with severity important for understanding the mechanisms responsible this heterogeneity and developing novel agents to prevent critical conditions. This study aimed elucidate modulations sphingolipids glycerophospholipids, which have been shown possess potent biological properties. Methods We measured serum sphingolipid...
Abstract Background Cryptococcosis is a notable infectious complication of liver transplantation. Currently, there no recommendation for screening serum cryptococcal antigen (CrAg) levels in solid organ transplant recipients. We aimed to explore the role CrAg recipients at an institution where posttransplant has been widely tested. Methods This retrospective study was conducted tertiary care center Japan. All with measured either or diagnostic testing least once after transplantation between...
To evaluate the efficacy and safety of nafamostat combined with favipiravir for treatment COVID-19.We conducted a multicenter, randomized, single-blind, placebo-controlled, parallel assignment study in hospitalized patients mild-to-moderate COVID-19 pneumonia. Patients were randomly assigned to receive alone (n = 24) or 21). The outcomes included changes World Health Organization clinical progression scale score, time improvement body temperature, oxygen saturation (SpO2).There was no...
We investigated the expression and function of a panel Toll-like receptors (TLRs) in human basophils. Basophil preparations constitutively expressed TLR2, TLR4, TLR9 TLR10 mRNAs (TLR4 > TLR2 >> TLR9, TLR10). Although TLR mRNA basophils was generally less prominent compared with those neutrophils monocytes, significantly higher levels TLR4 than eosinophils. Various ligands (Pam3Cys-Ser-Lys4, poly I:C, lipopolysaccharide, R-848, CpG DNA) were tested, but none affected level adhesion molecule...
This study aimed to compare the clinical characteristics and prognoses of central venous catheter-associated bloodstream infections (CVC-BSIs) with peripheral BSIs (PVC-BSIs).This retrospective observational was conducted between April 2011 March 2013 at a teaching hospital in Tokyo, Japan. Adult patients who developed CVC-BSIs PVC-BSIs more than 2 days after admission were included. Patients both excluded. Clinical obtained from medical records, 30-day all-cause mortality measured as...
The causative agents of recurrent Escherichia coli bacteremia can be genetically identical or discordant, but the differences between them remain unclear. This study aimed to explore these differences, with regard their clinical and microbiological features. Patients were recruited from a Japanese tertiary teaching hospital based on blood culture data incidence E. bacteremia. We compared patients' characteristics two groups (those discordant bacteremia) divided by result enterobacterial...
Among various complications of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2), renal complications, namely COVID-19-associated kidney injuries, are related to the mortality COVID-19.In this retrospective cross-sectional study, we measured sphingolipids and glycerophospholipids, which have been shown possess potent biological properties, using liquid chromatography-mass spectrometry in 272 urine samples collected longitudinally from 91 COVID-19...
Abstract Several clinical trials have shown that the humoral response produced by anti-spike antibodies elicited coronavirus disease 2019 (COVID-19) vaccines gradually declines. The kinetics, durability and influence of epidemiological factors on cellular immunity not been fully elucidated. We analyzed immune responses BNT162b2 mRNA in 321 health care workers using whole blood interferon-gamma (IFN-γ) release assays. IFN-γ, induced CD4 + CD8 T cells stimulated with severe acute respiratory...
ABSTRACT Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of B. isolates from CRBSIs remain understudied. Here, using advanced genomic techniques, we investigated diversity, phylogenetic relationships, patients with infections. We analyzed 28 group strains isolated blood samples at University Tokyo Hospital between 2005...
Abstract Background The epidemiology, microbiology, and clinical course of nontuberculous mycobacterial (NTM) infection among lung transplant recipients vary considerably by geographic location. Their data is still scarce. Methods This single-center retrospective study was conducted at one the major centers in Japan. Medical records microbiological test results all who underwent transplants between 2015 to 2023 were reviewed through December 31, 2023. Per institutional protocol, tracheal...
Abstract Background Clinical studies on clostridial bacteremia are scarce, and the factors associated with mortality have not been well investigated. Furthermore, distribution of Clostridium species varied among studies. To investigate epidemiology mortality, we conducted a study in Japan. Methods This multicenter, retrospective, observational was four Japanese hospitals. Patients whom spp. were detected blood cultures between 2007 2021 included. Only first episode infection included culture...
Invasive aspergillosis has an extremely high mortality rate. In Japan, micafungin, echinocandin drug that a new mechanism of action as antifungal agent and clinical effect against Aspergillus species, became available in 2002. However, little is known about its penetration into the central nervous system (CNS), or efficacy for treatment invasive CNS aspergillosis. We report 65-y-old female with diabetes mellitus who was treated micafungin. During treatment, micafungin concentrations were...
Abstract In Coronavirus disease 2019 (COVID-19) subjects, recent evidence suggests the presence of unique coagulation abnormalities. this study, we performed clot waveform analyses to investigate whether specific modulations are observed in COVID-19 subjects. We analyzed second derivative absorbance routine APTT tests using an ACL-TOP system. high frequencies abnormal patterns second-derivative curves that could be classified into early shoulder type, a late or biphasic maximum...
Klebsiella pneumoniae is a pathogen that causes pneumonia and urinary tract infection. Hypervirulent K. strains often show hypermucoviscosity, are of the K1 or K2 serotype, harbor rmpA magA genes. However, differences in prevalence with these hypervirulent characteristics between infection colonization status not well understood. Therefore, this study, we compared clinical microbiological isolated from urine sputum samples cases colonization.This retrospective study was conducted at tertiary...