A5103191048- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- Viral Infections and Outbreaks Research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
- Cancer-related molecular mechanisms research
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Metal complexes synthesis and properties
- MicroRNA in disease regulation
- Mosquito-borne diseases and control
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Vector-Borne Animal Diseases
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Synthesis and biological activity
- Bacteriophages and microbial interactions
- Bioactive Compounds and Antitumor Agents
- Click Chemistry and Applications
Air Force Medical University
2016-2025
Guangxi Normal University
2016-2025
Zhongda Hospital Southeast University
2025
Tang Du Hospital
2023-2024
Shanghai University
2024
Beijing Luhe Hospital Affiliated to Capital Medical University
2024
Qingdao University
2014-2024
Affiliated Hospital of Qingdao University
2016-2024
Taiyuan University of Science and Technology
2024
Shenzhen Bay Laboratory
2023
Enhanced aerobic glycolysis constitutes an additional source of energy for tumor proliferation and metastasis. Human papillomavirus (HPV) infection is the main cause cervical cancer (CC); however, associated molecular mechanisms remain poorly defined, as does relationship between CC glycolysis. To investigate whether HPV 16/18 E6/E7 can enhance in CC, expression was knocked down SiHa HeLa cells using small interfering RNA (siRNA). Then, glucose uptake, lactate production, ATP levels,...
Abstract Cervical cancer (CC) is the most common gynecologic malignancy, which seriously threatens health of women. Lipid metabolism necessary for tumor proliferation and metastasis. However, molecular mechanism relationship between CC lipid remains poorly defined. We revealed expression IGF2BP3 in exceeded adjacent tissues, was positively associated with stage using human tissue microarrays. The Cell Counting Kit-8, colony formation assay, 5-ethynyl-2′-deoxyuridine transwell assays,...
Abstract A substantial number of human epidemiological data, as well experimental studies, suggest that adverse maternal stress during gestation is involved in abnormal behavior, mental, and cognition disorder offspring. To explore the effect prenatal (PS) on hippocampal neurons, this study, we observed dendritic field pyramidal neurons CA3, examined concentration glutamate (Glu), detected expression synaptotagmin‐1 (Syt‐1) N ‐methyl‐ D ‐aspartate receptor 1 (NR1) hippocampus juvenile female...
To improve the selectivity, delivery, and activity of ferric (Fe) anticancer agents, we design prodrugs based on N-donor residues human serum albumin (HSA) carrier IIA subdomain. We synthesized six Fe(III) compounds derived from 2-hydroxy-1-naphthaldehyde thiosemicarbazone (7-12). HSA complex structure revealed that Fe compound binds to hydrophobic cavity in Lys199 His242 replace two Cl atoms compound, coordinating with Fe(3+). In vivo data 12 HSA-12 inhibit growth liver tumor has stronger...
Abstract Background Colorectal cancer (CRC) is one of the most common malignant tumors digestive system, ranking third for morbidity and mortality worldwide. At present, no effective control method available this type. In tumor cells, especially iron metabolization, necessary its growth proliferation. High levels are an important feature to maintain growth; however, overall mechanism remains unclear. Methods We used western blotting, immunohistochemistry (IHC) real-time quantitative PCR...
As a continuation of our endeavors in discovering metal-based drugs with cytotoxic and antimetastatic activities, herein, we reported the syntheses 11 new rhodium(III)–picolinamide complexes exploration their potential anticancer activities. These Rh(III) showed high antiproliferative activity against tested cancer cell lines vitro. The mechanism study indicated that Rh1 ([Rh(3a)(CH3CN)Cl2]) Rh2 ([Rh(3b)(CH3CN)Cl2]) inhibited proliferation by multiple modes action via cycle arrest,...
CX3CL1, also named fractalkine or neurotactin, is the only known member of CX3C chemokine family that can chemoattract several immune cells. CX3CL1 exists in both membrane-anchored and soluble forms, with each mediating distinct biological activities. signals are transmitted through its unique receptor, CX3CR1, primarily expressed microglia central nervous system (CNS). In CNS, acts as a regulator activation response to brain disorders inflammation. Recently, there has been growing interest...
Abstract Malignant tumors typically undergo an atavistic regression characterized by the overexpression of embryonic genes and proto-oncogenes, including a variety cancer/testis antigens (CTAs) that are testis-derived not expressed or in trace amounts somatic tissues. Based on this theory, we established new method to identify unknown CTAs, spermatogenic cells-specific monoclonal antibody-defined antigen (SADA) method. Using SADA method, identified BAP31 as novel CTA confirmed expression is...
The aim of the present research was to study protective effects and underlying mechanisms apigenin on<sc>d</sc>-galactose-induced aging mice.
Four mononuclear terpyridine complexes [Cu(H-La)Cl2]·CH3OH (1), [Cu(H-La)Cl]ClO4 (2), [Cu(H-Lb)Cl2]·CH3OH (3), and [Cu(H-Lb)(CH3OH)(DMSO)](ClO4)2 (4) were prepared fully characterized. Complexes 1-4 exhibited higher cytotoxic activity against several tested cancer cell lines especially BEL-7402 cells compared to cisplatin, they showed low toxicity towards normal human liver cells. ICP-MS detection indicated that the copper accumulated in mitochondria. Mechanistic studies demonstrated induced...
Natural killer (NK) cells play a critical role in antitumor immunity, and the activation of NK is regulated by series cell receptors. Here, we show that crosslinking CD226, an important receptor, with anti-CD226 mAb LeoA1 on NKL cells, expression several microRNA transmembrane tumor necrosis factor-α. Among them, miR-30c-1(*) was noticed because overexpression triggered upregulation factor-α enhanced cytotoxicity against hepatoma lines SMMC-7721 HepG2. Furthermore, proved inhibitory...
Cardiovascular disease (CVD) has been identified as the leading cause of premature deaths in rheumatoid arthritis (RA), accounting for about 40 to 50% all deaths. Macrophage inflammation is regarded a key point link two diseases. Recently, long non-coding RNAs (lncRNAs) have acknowledged regulator significantly. Here, we firstly found that lncRNA myocardial infarction associated transcript (lncRNA MIAT), crucial regulate CVD, expressed increasingly synovium and tissues collagen-induced (CIA)...
Nearly half a million women are diagnosed with cervical cancer (CC) each year, the incidence of CC stabilizing or rising in low-income and middle-income countries. Cancer cells use metabolic reprogramming to meet needs rapid proliferation, known as Warburg effect, but mechanism effect remains unclear. microRNAs (miRNAs) have wide range effects on gene expression diverse modes action, they regulate genes for reprogramming. Dysregulation miRNA leads abnormalities tumor promotes tumorigenesis...
The cellular immune response to Hantaan virus (HTNV) is incompletely understood, especially in humans.To investigate the immunity during acute HTNV infection, magnitude of CD4(+) and CD8(+) T cell responses nucleocapsid protein was quantitated by direct ex vivo interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot analysis, using an array overlapping peptides.We found that combined frequencies HTNV-specific cells at earliest available time point (5-8 days after fever onset) were...
Abstract BAP31 (B‐cell receptor‐associated protein 31) is an important regulator of intracellular signal transduction and highly expressed in several cancer tissues or testicular tissues. Our previous study had revealed that elevated plays a crucial role the progress metastasis cervical cancer. Even so, precise mechanism abnormal elevation has not been fully elucidated. We expression was mainly regulated by microRNA‐362 (miR‐362), which markedly downregulated negatively correlated with...
Hepatocellular carcinoma (HCC) patients are mostly diagnosed at an advanced stage, resulting in systemic therapy and poor prognosis. Therefore, the identification of a novel treatment target for HCC is important. B-cell receptor-associated protein 31 (BAP31) has been identified as cancer/testis antigen; however, BAP31 function mechanism action remain unclear. In this study, was demonstrated to be upregulated correlated with clinical stage. overexpression promoted cell proliferation colony...
A highly specific and sensitive microplate chemiluminescent enzyme immunoassay (CLEIA) was established validated for the detection of staphylococcal enterotoxin B (SEB). pair monoclonal antibodies (mAbs) that recognizes different epitopes SEB selected from 20 SEB-specific mAbs, experimental conditions were examined optimized development CLEIA. This method exhibited high performance with a dynamic range 0.01−5 ng/mL, measured limit (LOD) 0.01 ng/mL. Intra- interassay coefficient variations...