A5075679677- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Malaria Research and Control
- Lymphoma Diagnosis and Treatment
- Invertebrate Immune Response Mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Microtubule and mitosis dynamics
- Lung Cancer Treatments and Mutations
- Mosquito-borne diseases and control
- AI in cancer detection
- Brain Tumor Detection and Classification
- Cell Adhesion Molecules Research
- Endometrial and Cervical Cancer Treatments
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
- Sperm and Testicular Function
- HIV Research and Treatment
- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Signaling Pathways in Disease
- Cellular transport and secretion
- Multiple Myeloma Research and Treatments
University of Leicester
2016-2025
University of Nottingham
2012-2025
Genomics (United Kingdom)
2025
Queen's Medical Centre
2012-2024
Leicester Royal Infirmary
2013-2023
Cancer Research UK
2015-2016
Medical Research Council
2009
Up to 30% of patients with breast cancer relapse after primary treatment. There are no sensitive and reliable tests monitor these detect distant metastases before overt recurrence. Here, we demonstrate the use personalized circulating tumor DNA (ctDNA) profiling for detection recurrence in cancer.
The purpose of this study was to directly compare mutation profiles in multiple single circulating tumor cells (CTC) and cell-free DNA (cfDNA) isolated from the same blood samples taken patients with metastatic breast cancer (MBC). We aimed determine whether cfDNA would reflect heterogeneity observed 40 CTCs.
Biomarkers in breast cancer to monitor minimal residual disease have remained elusive. We hypothesized that genomic analysis of circulating free DNA (cfDNA) isolated from plasma may form the basis for a means detecting and monitoring cancer. profiled 251 genomes using Affymetrix SNP 6.0 arrays determine copy number variations (CNVs) loss heterozygosity (LOH), comparing 138 cfDNA samples with matched primary tumor normal leukocyte 65 patients eight healthy female controls. Concordance...
Circulating nucleic acids (CNAs) are under investigation as a liquid biopsy in cancer. However there is wide variation blood processing and methods for isolation of circulating free DNA (cfDNA) microRNAs (miRNAs). Here we compare the extraction efficiency reproducibility 4 commercially available kits cfDNA 3 miRNA using spike-in reference templates. We also effects increasing time between venepuncture centrifugation differential force on recovery CNAs. was quantified by TaqMan qPCR targeted...
Abstract BACKGROUND Activating mutations in the estrogen receptor 1 (ESR1) gene are acquired on treatment and can drive resistance to endocrine therapy. Because of spatial temporal limitations needle core biopsies, our goal was develop a highly sensitive, less invasive method detecting activating ESR1 via circulating cell-free DNA (cfDNA) tumor cells as “liquid biopsy.” METHODS We developed targeted 23-amplicon next-generation sequencing (NGS) panel for detection hot-spot ESR1,...
Aim: This paper proposes a novel alcoholism identification approach that can assist radiologists in patient diagnosis. Method: AlexNet was used as the basic transfer learning model. The global rate small, at 10-4, and iteration epoch number 10. factor of replaced layers 10 times larger than transferred layers. We tested five different replacement configurations learning. Results: experiment shows best performance achieved by replacing final fully connected layer. Our method yielded...
Reversible protein phosphorylation regulated by kinases and phosphatases controls many cellular processes. Although essential functions for the malaria parasite kinome have been reported, roles of most (PPs) during Plasmodium development are unknown. We report a functional analysis berghei phosphatome, which exhibits high conservation with P. falciparum phosphatome comprises 30 predicted PPs differential distinct expression patterns various stages life cycle. Gene disruption reveals that...
Breast cancer tissues are heterogeneous and show diverse somatic mutations copy number alterations (CNAs). We used a novel targeted next generation sequencing (NGS) panel to examine cell-free DNA (cfDNA) detect gene amplification in women with metastatic breast (MBC).
Abstract Background Breast cancer (BC) is the most common in women, and despite introduction of new screening programmes, therapies monitoring technologies, there still a need to develop more useful tests for treatment response inform clinical decision making. The purpose this study was compare circulating cell-free DNA (cfDNA) tumour cells (CTCs) with conventional breast blood biomarkers (CA15-3 alkaline phosphatase (AP)) as predictors prognosis patients metastatic (MBC). Methods One...
<italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">(Aim)</i> Breast cancer is the most common in women and second worldwide. With rapid advancement of deep learning, early stages breast development can be accurately detected by radiologists with help artificial intelligence systems. xmlns:xlink="http://www.w3.org/1999/xlink">(Method)</i> Based on mammographic imaging, a mainstream clinical screening technique, we present diagnostic system for...
PURPOSE Here, we report the sensitivity of a personalized, tumor-informed circulating tumor DNA (ctDNA) assay (Signatera) for detection molecular relapse during long-term follow-up patients with breast cancer. METHODS A total 156 primary cancer were monitored clinically up to 12 years after surgery and adjuvant chemotherapy. Semiannual blood samples prospectively collected, analyzed retrospectively detect residual disease by ultradeep sequencing using ctDNA assays, developed from whole-exome...
Abstract Introduction The stromal microenvironment has a profound influence on tumour cell behaviour. In tumours, the extracellular matrix (ECM) composition differs from normal tissue and allows novel interactions to function. ECM protein tenascin-C (TNC) is frequently up-regulated in breast cancer we have previously identified two isoforms – one containing exon 16 (TNC-16) exons 14 plus (TNC-14/16). Methods present study analysed functional significance of this altered TNC isoform profile...
Human epidermal growth factor receptor 2 (HER2) is amplified and overexpressed in 20–25% of breast cancers. This study investigated circulating free DNA (cfDNA) for detection HER2 gene amplification patients with cancer. Circulating was extracted from plasma unselected primary cancer (22 before surgery 68 following treatment), 30 metastatic 98 female controls using the QIAamp Blood Mini Kit (Qiagen). The ratio to an unamplified reference (contactin-associated protein 1 (CNTNAP1)) measured...
Protein phosphorylation and dephosphorylation (catalysed by kinases phosphatases, respectively) are post-translational modifications that play key roles in many eukaryotic signalling pathways, often deregulated a number of pathological conditions humans. In the malaria parasite Plasmodium, functional insights into its kinome have only recently been achieved, with over half being essential for blood stage development another 14 sexual mosquito transmission. However, functions any plasmodial...
Cell-cycle progression and cell division in eukaryotes are governed part by the cyclin family their regulation of cyclin-dependent kinases (CDKs). Cyclins very well characterised model systems such as yeast human cells, but surprisingly little is known about number role Plasmodium, unicellular protozoan parasite that causes malaria. Malaria proliferation differs from many eukaryotes. During its life cycle it undergoes two types mitosis: endomitosis asexual stages an extremely rapid mitotic...
Traditional deep learning methods are suboptimal in classifying ambiguity features, which often arise noisy and hard to predict categories, especially, distinguish semantic scoring. Semantic scoring, depending on logic implement evaluation, inevitably contains fuzzy description misses some concepts, for example, the ambiguous relationship between normal probably always presents unclear boundaries (normal-more likely normal-probably normal). Thus, human error is common when annotating images....
Eukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. The genus Plasmodium, the causative agent of malaria, displays remarkable aspects nuclear division throughout its lifecycle meet some peculiar unique challenges DNA segregation. parasite undergoes atypical endomitosis endoreduplication with an intact membrane intranuclear mitotic spindle. To understand these diverse modes Plasmodium division, we...
Despite the increasing incidence of endometrial cancer (EC) worldwide and poor overall survival patients who recur, no reliable biomarker exists for detecting monitoring EC recurrence progression during routine follow-up. Circulating tumor DNA (ctDNA) is a sensitive method activity stratifying that are likely to respond therapy. As pilot study, we investigated utility ctDNA in 13 patients, using targeted next-generation sequencing (tNGS) personalized assays. Using tNGS, at least one somatic...
Abstract The Aurora family of kinases orchestrates chromosome segregation and cytokinesis during cell division, with precise spatiotemporal regulation its catalytic activities by distinct protein scaffolds. Plasmodium spp., the causative agents malaria, are unicellular eukaryotes three unique highly divergent aurora-related (ARK1-3) that essential for asexual cellular proliferation but lack most canonical scaffolds/activators. Here we investigate role ARK2 sexual rodent malaria berghei ,...