A5022848088- Lung Cancer Treatments and Mutations
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA modifications and cancer
- Bacterial Identification and Susceptibility Testing
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Eosinophilic Disorders and Syndromes
- Antimicrobial Resistance in Staphylococcus
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Lung Cancer Research Studies
- Enterobacteriaceae and Cronobacter Research
- Colorectal Cancer Treatments and Studies
- DNA Repair Mechanisms
- Kruppel-like factors research
- Glioma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Head and Neck Cancer Studies
- Clostridium difficile and Clostridium perfringens research
- Salivary Gland Tumors Diagnosis and Treatment
- Gut microbiota and health
Institut Gustave Roussy
2010-2025
Centre National de la Recherche Scientifique
2004-2023
Université d'Angers
2017-2023
Inserm
2021-2023
Centre Hospitalier Universitaire d'Angers
2019-2023
Université Bretagne Loire
2022
Université Paris-Saclay
2015-2021
Université Paris-Sud
2015
Jilin University
2013
Washington University in St. Louis
2013
Diffuse intrinsic pontine glioma (DIPG) is the most severe paediatric solid tumour, with no significant therapeutic progress made in past 50 years. Recent studies suggest that diffuse midline glioma, H3-K27M mutant, may comprise more than one biological entity. The aim of study was to determine clinical and variables impact their prognosis. Ninety-one patients classically defined DIPG underwent a systematic stereotactic biopsy were included this observational retrospective study. Histone H3...
The excision repair cross-complementation group 1 (ERCC1) protein is a potential prognostic biomarker of the efficacy cisplatin-based chemotherapy in non–small-cell lung cancer (NSCLC). Although several ongoing trials are evaluating level expression ERCC1, no consensus has been reached regarding method for evaluation.
The mechanisms of action and resistance to trastuzumab deruxtecan (T-DXd), an anti-HER2-drug conjugate for breast cancer treatment, remain unclear. phase 2 DAISY trial evaluated the efficacy T-DXd in patients with HER2-overexpressing (n = 72, cohort 1), HER2-low 74, 2) HER2 non-expressing 40, 3) metastatic cancer. In full analysis set population 177), confirmed objective response rate (primary endpoint) was 70.6% (95% confidence interval (CI) 58.3-81) 1, 37.5% CI 26.4-49.7) 29.7% 15.9-47) 3....
Abstract Obtaining representative human colon cancer cell lines from fresh tumors is technically difficult. Using 32 tumor fragments patients with cancer, the present study shows that prior xenograft leads to more efficient line establishment compared direct (P < 0.05). From 26 specimens, we successfully established 20 xenografts in nude mice (77%); among 19 of these xenografts, 9 (47%) led lines, including four liver metastases. Only 3 31 specimens (9.7%) grew immediately vitro, and...
Fibromatosis comprises distinct clinical entities, including sporadic extra-abdominal fibromatosis, which have a high tendency for recurrence, even after adequate resection. There are no known molecular biomarkers of local recurrence. We searched β-catenin mutations in European multicentre series fibromatosis tumours to relate mutational status disease outcome. Direct sequencing exon 3 gene was performed 155 frozen tissues from all topographies. Correlation outcome with mutation rate and...
Twenty-six strains of methicillin-resistant Staphylococcus aureus with different pulsed-field gel electrophoresis fingerprints were tested by random amplified polymorphic DNA assay three primers, resulting in 15 to 20 fingerprints. By summing the results for number increased 25, but two could not be differentiated. We conclude that remains best method typing S. strains.
Once initiated, invasion of trophoblast cells must be tightly regulated, particularly in early pregnancy. The mechanisms necessary for the and migration are thought to related those involved invasive metastatic properties cancer cells. Quantitative PCR was used measure, cells, transcriptional expression profiles four genes, INSL4, BRMS1, KiSS-1 KiSS-1R, reported implicated tumor metastasis. Laser capture microdissection purification demonstrate that, as already known KiSS-1R expressed by...
Animal studies indicate that the use of replication-deficient adenovirus for human gene therapy is limited by host antivector immune responses result in transient recombinant protein expression and blocking transfer when rechallenged. Therefore, we have examined to an adenoviral vector beta-galactosidase four patients with lung cancer given a single intratumor injection 10(9) plaque-forming units adenovirus. The was expressed day-8 tumor biopsies from all at variable levels. Recombinant...
ABSTRACT PCR assays for the diagnosis of systemic candidiasis can be performed either on serum or whole blood, but results obtained with two kinds samples have never been formally compared. Thus we designed a nested assay in which five specific inner pairs primers were used to amplify targets rRNA genes Candida albicans , C. tropicalis parapsilosis krusei and glabrata . In vitro, lower limit detection each was 1 fg purified DNA from corresponding species. rabbits candidemia 120 minutes’...
Despite vigorous efforts at curbing tobacco consumption and aggressive combined-modality treatment programs, both the incidence of mortality from lung cancer have remained virtually unchanged in last 10 years. More effective innovative therapies are clearly needed. The direct transfer into tumor cells suppressor genes or toxic gene products that specifically promote cell death spare nonmalignant is a potentially novel anticancer approach should be investigated. Purpose: On basis compelling...
Non-functioning pituitary adenomas (NFPAs) may be locally invasive. Markers of invasiveness are needed to guide patient management and particularly the use adjuvant radiotherapy. To examine whether invasive NFPAs display a specific gene expression profile relative non-invasive tumors, we selected 40 (38 gonadotroph type) classified them as (n=22) or (n=18) on basis magnetic resonance imaging surgical findings. We then performed pangenomic analysis with 44k Agilent human whole genome...
Circulating senescent CD8+ T (T8sen) cells are characterized by a lack of proliferative capacities but retain cytotoxic activity and have been associated to resistance immunotherapy in patients with advanced non-small cell lung cancer (aNSCLC). We aimed better characterize T8sen determine which factors were their accumulation aNSCLC. higher expression SA-βgal the transcription factor T-bet, confirming status. Using whole virome profiling, cytomegalovirus (CMV) was only virus T8sen. CMV...
Targeting adenovirus encoding therapeutic genes to specific cell types has become a major goal in gene therapy. Coxsackievirus and receptor (CAR) alpha(V) integrins have been identified as the primary surface components that interact with type 5 (Ad5)-based vectors during vitro transduction. Redirecting Ad5-based requires abrogation of natural interaction between viral capsid its cellular receptors simultaneous introduction new binding specificity into capsid. To abrogate native Ad5 tropism,...
Sixty vancomycin-resistant vanA mutant Enterococcus faecium (VRE) isolates, collected during a 40-month period from 48 patients hospitalized in French Cancer Referral Center, were typed by using random amplified polymorphic DNA (RAPD), and the results compared with those previously obtained typing SmaI pulsed-field gel electrophoresis (PFGE), which is currently recognized as "gold standard." The discriminating power of RAPD typing, seven primers 11 combinations primers, was tested on 18...
ERCC1 (excision repair cross-complementation group 1) plays essential roles in the removal of DNA intrastrand crosslinks by nucleotide excision repair, and that interstrand Fanconi anemia (FA) pathway homology-directed processes (HDR). The function thus impacts on damage response (DDR), particularly anticancer therapy when damaging agents are employed. expression has been proposed as a predictive biomarker to platinum-based therapy. However, assessment clinical samples is complicated...
Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (SCC) is associated with favorable survival. The purpose of this study was to evaluate the prevalence and prognostic significance HPV infection through both p16 expression status oncogenic DNA viral load.A retrospective chart review conducted on all patients treated for SCC between January 2007 June 2009. P16 by immunohistochemistry load quantitative polymerase chain reaction (qPCR) were evaluated routine pretreatment...
Secondary solid tumors are rare events occurring in patients who underwent allogeneic marrow transplantation for aplastic anemia and Fanconi's anemia. Human herpes virus 8 (HHV8), Epstein-Barr (EBV), human papillomaviruses (HPV) sequences have been found squamous cell carcinoma (SCC) organ transplant recipients. The tumor suppressor gene p53 has strongly linked to the occurrence of SCC nonimmunocompromised population.In eight with SCC, we searched HHV8, EBV, varicella zoster virus,...