A5088054109- Effects and risks of endocrine disrupting chemicals
- Animal Genetics and Reproduction
- Health, Environment, Cognitive Aging
- Sperm and Testicular Function
- Reproductive biology and impacts on aquatic species
- Reproductive Biology and Fertility
- Carcinogens and Genotoxicity Assessment
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Toxic Organic Pollutants Impact
- Animal testing and alternatives
- Birth, Development, and Health
- Molecular Biology Techniques and Applications
- Per- and polyfluoroalkyl substances research
- Microplastics and Plastic Pollution
- Insect behavior and control techniques
- RNA Research and Splicing
- Plant and animal studies
- Chromosomal and Genetic Variations
- Horticultural and Viticultural Research
- Genomics and Chromatin Dynamics
- Agricultural and Rural Development Research
- Blood Pressure and Hypertension Studies
- Selenium in Biological Systems
- Cannabis and Cannabinoid Research
Constantine the Philosopher University in Nitra
2016-2025
University of Ss. Cyril and Methodius in Trnava
2022
Corvinus University of Budapest
2022
University of Constantine 3
2014
This study aims to explore the association between sleep patterns in children and their exposure phthalates assess potential health implications. Participants (n = 60) completed Pittsburgh Sleep Quality Index (PSQI) questionnaire. Consumer behavior scores (CBS) were calculated. The Tanner scale was used monitor stages of puberty. First-morning urine samples analyzed by high-performance liquid chromatography-tandem mass spectrometry. average duration 8 h 44 min, with boys sleeping...
We studied occupational exposure to phthalates from first-morning-void urine sample of hairdressing apprentices by HPLC-MS/MS analyses and association with health status. Metabolites mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), mono ethyl (MEP), mono- n-butyl (MnBP) mono-iso-butyl (MiBP) were detected in all samples, followed metabolites mono(2-ethyl-5-oxohexyl) (MEOHP) mono(2-etylhexyl) (MEHP) occurring 97.06% 86.76% respectively. Positive associations for females observed between MnBP...
Many toxic substances in the workplace can modify human health and quality of life there is still insufficient data on respiratory outcomes adults exposed to phthalates. The aim this work was assess waste management workers from Nitra region Slovakia (n = 30) extent exposure phthalates health-related outcomes. Four urinary phthalate metabolites mono(2-ethylhexyl) (MEHP), monobutyl (MnBP), monoethyl (MEP) monoisononyl (MiNP) were determined by high-performance liquid chromatography with mass...
Despite increasing attention to the occupational risk of firefighters, little is known about phthalate exposure. In our study, we detected mono-isobutyl (MiBP), mono-n-buthyl (MnBP), mono(2-ethyl-5-hydroxyhexyl) (5OH-MEHP), mono(2-ethyl-5-carboxypentyl) (5cx-MECPP), and mono(2-ethyl-5-oxohexyl) (5oxo-MEHP) in each urine sample. We positive association between MnBP, MiBP, mono-2-ethylhexyl (MEHP), 5OH-MEHP, 5oxo-MEHP, 5cx-MECPP, mono-isononyl (MiNP), sum low (∑LMWP) high molecular-weight...
Abstract There is growing evidence that endocrine disruptive chemicals have deleterious effects on sexual and reproductive function. To examine subjective functions in human females their relationship to postnatal phthalate exposure perinatal androgenization, a Sexuality Score (SS) was established from first-stage survey questionnaire of function filled out by female university students ( n = 68; average age 25.23 ± 5.17 years; rural 25.51 6.74 vs. urban 25.85 1.43 years). Seventeen...
Over the years eastern Slovakia has been subject to consistent monitoring of high levels polychlorinated biphenyls (PCBs) in both environment and human populations attributed former production PCBs at Chemko Strážske plant. We aimed investigate extent which dietary habits residential location could affect concentrations blood serum samples subjects.
The objectives of the study were to examine occupational exposure phthalates hairdressing apprentices from Slovakia (n = 74, 89.2% females; 10.8% males), outcomes related body composition and pulmonary functions. We used high-performance liquid chromatography tandem mass spectrometry quantified urinary concentration phthalates. Pulmonary function test (PFT), anthropometric measurements, questionnaire also conducted. observed a decrease % predicted values forced vital capacity (FVC% PV)...
Background: Occupational health hazards associated with phthalate exposure among nurses are still not well understood. Methods: We used high-performance liquid chromatography and tandem mass spectrometry to analyze phthalates. Anthropometric measurements questionnaires were conducted. Results: observed associations between mono-benzyl (MBzP) body index (BMI), hip circumference (HC), waist (WC), height ratio (WHtR), fat (FMI), visceral content, BMI risk (HIrisk), adjusted consumer behavior...
Phthalates like endocrine disrupting compounds are ubiquitous environmental contaminants. Long term exposure to these chemicals at the work place has adverse effect on human health and usually leads diseased conditions. The aim of our study was assess, by biological monitoring, occupational di-(2-ethylhexyl) phthalate (DEHP), di-n-butyl (DnBP), diethyl (DEP) diisononyl (DiNP) occupationally exposed group i.e. workers from plastic manufactory with division films composites injection moulding...
Adverse birth outcomes present risk factors resulting in neonatal morbidity and mortality. Sufficient maternal hormonal concentrations are crucial for normal foetal development. Previous studies have shown a relationship between phthalate exposure levels during pregnancy. This study aims to investigate if anthropometric parameters associated with endocrine the ≤15th week of gestation third trimester pregnancy concerning pregnant women from Nitra, Slovakia. We used high-performance liquid...
Phthalates are mainly used as plasticizers and associated inter alia with adverse effects on reproductive functions. While more national programs in Europe have started monitoring internal exposure to phthalates its substitute 1,2-Cyclohexanedicarboxylic acid (DINCH), the comparability of results from such existing human biomonitoring (HBM) studies across is challenging. They differ widely time periods, study samples, degree geographical coverage, design, analytical methodology, biomarker...
Based on toxicological evidence, children’s exposure to phthalates may contribute altered neurodevelopment and abnormal regulation of brain-derived neurotrophic factor (BDNF). We analyzed data from five aligned studies the Human Biomonitoring for Europe (HBM4EU) project. Ten phthalate metabolites protein BDNF levels were measured in urine samples 1148 children aged 6–12 years Italy (NACII-IT cohort), Slovakia (PCB-SK Hungary (InAirQ-HU cohort) Norway (NEBII-NO). Serum was also available 124...
Abstract The aim of the present study was to investigate role RNA polymerase I (RPI) transcription in nucleolar development during major transcriptional activation (MTA) cattle. Late eight‐cell embryos were cultured absence (control group) or presence actinomycin D (AD) (RPI inhibition, AD 0.2 µg/ml; total 2.0 µg/ml). four‐cell late stage µg/ml (MTA prevention, ADLT (long‐term inhibition group). Embryos processed for autoradiography, transmission electron microscopy, fluorescent situ...
The immediate events of genomic reprogramming at somatic cell nuclear transfer (SCNT) are to high degree unknown. This study was designed evaluate the and nucleolar changes during first cycle. Bovine SCNT embryos were produced from starved bovine fibroblasts fixed 0.5, 1, 2, 3, 4, 8, 12, 16 h postactivation (hpa). Parthenogenetic (PA) used as control. PA processed for lacmoid staining, autoradiography, transmission electron microscopy (TEM), immunofluorescence localization of: upstream...
The aim of this study was to evaluate the turkey spermatozoa motility in vitro conditions and prove effect different incubation – diluents, temperature age birds. Spermatozoa were obtained from adult turkey's line Big 6, parameters evaluated using a computer-assisted semen analyzer (CASA) system. Significant decrease at laboratory (22°C) detected time 0 (94.15%) till 180 minutes (53.91%). At cool media (5°C), difference lower (95.41 78.86%, respectively), differences significant 30 minutes....
Initially, development of the zygote is under control oocyte ooplasm. However, it presently unknown if and to what extent ooplasm able interact with a transferred somatic cell from another species in context interspecies nuclear transfer (SCNT). Here, one-cell stage embryos were processed at different points time post activation (2 hpa, 4 8 12 hpa) for detailed nucleolar analysis by TEM, immunofluorescence visualization proteins related transcription (UBF) processing (fibrillarin). Bovine...
Copper as essential trace element is obligatory in many biological processes. Nevertheless higher concentrations of this metal are highly toxic for organisms at different levels and course spermatozoa. The aim present work was to examine impact copper sulphate on turkey spermatozoa motility parameters (line Big 6) during vitro incubation 41°C. Four concentration CuSO4: M1 – 6.25 μg.ml-1; M2 12.5 M3 25.0 M4 50.0 μg.ml-1 were investigated. evaluated using the Computer Assisted Semen Analyzer...
Our study aimed to establish the best prediction equation for different age ranges in estimating Glomerular Filtration Rate (GFR) clinical practice Slovakia. The GFR by 24-hour creatinine clearance (Ccr) and estimated (eGFR) using Cockcroft-Gault (CG), four-variable Modification of Diet Renal Disease (MDRD4) Chronic Kidney Epidemiology Collaboration (CKD-EPI) equations were obtained adults aged 30-80 (n=433, 10-years intervals). correlation between these Ccr was evaluated. Errors detected...