A5076003741- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Cervical and Thoracic Myelopathy
- Cancer, Lipids, and Metabolism
- Nanoparticle-Based Drug Delivery
- Spine and Intervertebral Disc Pathology
- Lattice Boltzmann Simulation Studies
- Pulmonary Hypertension Research and Treatments
- Chemical Synthesis and Analysis
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Protease and Inhibitor Mechanisms
- Gastrointestinal Tumor Research and Treatment
- Sarcoma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Mesenchymal stem cell research
- Nanoplatforms for cancer theranostics
- Immune cells in cancer
- Venous Thromboembolism Diagnosis and Management
- Extracellular vesicles in disease
- RNA modifications and cancer
- Cancer, Stress, Anesthesia, and Immune Response
- Scoliosis diagnosis and treatment
First Affiliated Hospital of Gannan Medical University
2013-2025
Wenzhou Medical University
2024
Ruian People's Hospital
2024
Sun Yat-sen University
2012
Deep vein thrombosis (DVT) is a common type of venous thrombosis. Successful resolution DVT-related thrombi important in the treatment DVT. Endothelial progenitor cells (EPCs) have emerged as promising therapeutic choice for thrombus resolution; however, clinical application EPCs faces many challenges. In present study, expression miR-582, miR-195 and miR-532 under hypoxic or normoxic conditions was measured using quantitative real-time PCR analysis (qRT-PCR) results showed that increased...
CD133-positive cancer stem cells in colon are resistant to conventional chemotherapy. The aim of the present study was investigate effect celecoxib, a COX-2 inhibitor, on CD133 expression HT29 and DLD1 cells. were treated with celecoxib using different concentrations duration. detected by flow cytometry, Western blotting, immunofluorescence, quantitative real-time PCR. Wnt signaling pathway activity measured luciferase assay gene changes monitored microarray analysis. showed significantly...
Objective Previous research has demonstrated that metabolites play a significant role in modulating disease phenotypes; nevertheless, the causal association between and malignant malignancies of bones joint cartilage (MNBAC)has not been fully elucidated. Methods This study used two-sample Mendelian randomization (MR) to explore correlation 1,400 MNBAC. Data from recent genome-wide studies (GWAS) involving 8,299 individuals were summarized. The GWAS summary data for acquired IEU Open...
Deep vein thrombosis (DVT) is a complex multifactorial vascular disease characterized by abnormal blood stasis and coagulation within the deep veins, primarily occurring in lower limbs. This pathological condition not only causes local circulatory disruption but also has potential to trigger life-threatening pulmonary embolism through thrombus detachment, thus posing major threat human health. Toll - like receptors (TLRs), essential components of innate immune system, have been increasingly...
NLRP3 (NACHT, LRR, and PYD domains-containing protein 3) inflammasome is a critical regulator of inflammatory responses in the body closely associated with processes various diseases. In recent years, research has increasingly focused on role venous thromboembolism (VTE). Venous common potentially fatal vascular disease complex pathophysiology involving multiple cellular molecular pathways. The activates caspase-1 downstream, facilitating maturation secretion pro-inflammatory cytokines such...
Deep vein thrombosis (DVT) is a prevalent clinical venous thrombotic condition that often manifests independently or in conjunction with other ailments. Thrombi have the propensity to dislodge into circulatory system, giving rise complications such as pulmonary embolism, thereby posing significant risk patient. Virchow proposed blood stagnation, alterations vessel wall and hypercoagulation are primary factors contributing development of thrombosis. Vascular endothelial cells (VECs)...
Osteosarcoma (OS) is a primary bone tumor with high malignancy and poor prognosis. Ferroptosis plays crucial role in OS. This study aimed to evaluate the effects of Ankyrin 1 (ANK1) on OS investigate its specific mechanisms. Microarray datasets related "osteosarcoma" were selected for this study. Relevant hub genes identified through bioinformatics analysis. Transfected U-2OS MG-63 cells used vitro experiments. The ANK1 overexpression cell viability, migration, invasion determined CCK-8,...
Purpose: MicroRNAs play an important role in osteosarcoma (OS) development and progress. Although miR-1253 was considered as a tumor-inhibitor some cancers, it’s function the OS is not clear. Methods: In our study, we examined expression of cells osteoblast using quantitative real-time PCR. The proliferation measured by BrdU assay, performed transwell to detect migration invasion cells. Meanwhile, EMT proteins were tested western blot. We used Bioinformatics predict target genes found out...
Abstract A potential use of small extracellular vesicles (sEVs) for diagnostic and therapeutic purposes has recently generated a great interest. sEVs, when purified directly from various tissues with proper procedures, can reflect the physiological pathological state organism. However, quality sEV is affected by many factors during isolation, including separation cell debris, enzymes tissue digestion, storage tissues. In present study, we established an assay isolation purification liver...
Osteoporosis, characterized by decreased mineral density and bone mass, is triggered various detrimental factors often causes further complications, including fractures. Aberrant expression of microRNAs (miRs) has been associated with the pathogenesis osteoporosis. Recently, miR-142 was reported to be downregulated in osteoblasts; however, underlying mechanism mediating development osteoporosis remains unclear. In present study, high glucose induced downregulation mRNA promoted apoptosis...
Background CD133 is a marker that identifies/enriches cancer stem cell implicated in tumor initiation. We hypothesize changes the mRNA expression levels and vascular endothelial growth factor (VEGF) may correlate response GIST. Methodology/Principal Findings After informed consent, we obtained peripheral blood samples from 24 evaluable patients with gastrointestinal stromal tumors (GIST). There were 7 -paired before after treatment, measured by real time RT-PCR method ELISA. All measurements...
Objective: The aim of this study was to investigate the adenovirus-mediated human bone morphogenetic protein-2 (Ad-BMP-2) transfection rabbit marrow mesenchymal stem cells (rBMSCs), which, together with hydroxyapatite nanocomposite (Nano-HA), were used construct tissue-engineered in vitro.
Effective delivery of therapeutic agents for solid tumour treatment is impeded by multiple obstacles, which causes impaired penetration to deep avascular tissue that exists in a hypoxic immune cold environment.
Abstract Background This study was undertaken to establish a rat bipedal walking model of cervical kyphosis (CK) associated with chronic forward flexed neck and assess the effects on endplate chondrocytes. Methods Forty-eight 1-month-old Sprague-Dawley rats were randomly divided into 3 groups: group ( n = 16), normal 16). Cervical curves analyzed lateral spine X-ray using Harrison’s posterior tangent method before experiment at 2-week intervals for 6-week period. Histologic changes in...