A5100360772- Drug Transport and Resistance Mechanisms
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Circular RNAs in diseases
- Liver physiology and pathology
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Genetic factors in colorectal cancer
- Epigenetics and DNA Methylation
- Gastric Cancer Management and Outcomes
- Liver Disease Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related gene regulation
- Estrogen and related hormone effects
- TGF-β signaling in diseases
- Fibroblast Growth Factor Research
- Cancer Mechanisms and Therapy
- Cellular Mechanics and Interactions
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Kruppel-like factors research
- Colorectal Cancer Treatments and Studies
- Pomegranate: compositions and health benefits
- Peptidase Inhibition and Analysis
Harbin Medical University
2015-2025
Fourth Affiliated Hospital of Harbin Medical University
2015-2025
Northwest University
2025
Air Force Medical University
2025
Tongji Hospital
2018-2024
Huazhong University of Science and Technology
2018-2024
University of Macau
2023
First Affiliated Hospital of Zhengzhou University
2010-2021
Tongji University
2010-2018
Shanghai Tenth People's Hospital
2011-2018
Circular RNAs (circRNAs) are a novel type of noncoding and play important roles in tumorigenesis, including gastric cancer (GC). However, the functions most circRNAs remain poorly understood. In our study, we aimed to investigate new circRNA circ-DONSON GC progression. The expression tissues adjacent normal was analyzed by bioinformatics method, qRT-PCR, Northern blotting situ hybridization (ISH). effects on cell proliferation, apoptosis, migration invasion were measured using CCK8, colony...
Target-based metabolomics, focused on a subset of metabolites representative key pathways, is valuable tool for assessing metabolic changes resulting from genetic mutation, altered gene expression, and protein dysfunction in given disease state or as consequence an environmental perturbation, such pharmaceutical. However, simultaneously analyzing hundreds endogenous presents challenge because their diverse chemical structures properties. In this study, we report high-throughput, sensitive,...
Breast cancer represents the second leading cause of cancer-related deaths among women worldwide and preventive therapy could reverse or delay devastating impact this disease. Ellagic acid (EA), a dietary flavonoid polyphenol which is present in abundance pomegranate, muscadine grapes, walnuts strawberries, has been shown to inhibit cells proliferation induce apoptosis. Here, we investigated growth inhibitory effects EA on MCF-7 breast cells. In study, first found that inhibits mainly...
Fibroblast growth factor (FGF) 15 (human homolog, FGF19) is an endocrine FGF highly expressed in the small intestine of mice. Emerging evidence suggests that FGF15 critical for regulating hepatic functions; however, role liver regeneration unclear. This study assessed whether altered knockout (KO) mice following 2/3 partial hepatectomy (PHx). The results showed KO had marked mortality, with survival rate influenced by genetic background. Compared wild-type mice, displayed extensive necrosis...
Abstract The poor prognosis of ovarian cancer is mainly due to metastasis, and the specific mechanism underlying metastasis not clear. Ascites-derived exosomes (ADEs) play an important role in progression cancer, but unknown. Here, we found that ADEs promoted only vitro also vivo. This promotive function was based on epithelial–mesenchymal transition (EMT) cells. Bioinformatics analysis RNA sequencing microarray data indicated miR-6780b-5p may be key microRNA (miRNA) facilitates metastasis....
To investigate possibility and clinical application of fecal calprotectin in determining disease activity ulcerative colitis (UC).The enzyme-linked immunosorbent assay (ELISA) was used to measure the concentrations feces obtained from 66 patients with UC 20 controls. C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), acid glycoprotein (AGP) were also measured compared UC. The determined by Sutherland criteria.The concentration active significantly higher than that inactive...
CDK4/6i, the first-line drug for treating ERα-positive breast cancer, significantly improves clinical outcomes. However, CDK4/6i resistance often develops and remains a major hurdle, underlying mechanisms remain challenging to fully investigate. Here, we used Genome-wide CRISPR/Cas9 library screening combined with single-cell sequencing screen molecules mediating identified METTL14 as determinant of sensitivity. Clinical samples datasets were analyzed in vitro vivo experiments performed...
Background Colorectal adenocarcinoma (COAD) is a prevalent malignant tumor associated with high mortality rate. Within the microenvironment, CD8 + T cells play pivotal role in anti-tumor immune response within human body. Fibrosis directly and indirectly affects therapeutic of immunotherapy. However, significance regulatory genes tumor-associated fibrosis cell infiltration remains uncertain. Therefore, it imperative to identify biomarkers prognostic value elucidate precise fibrosis. Methods...
Extrahepatic cholangiocarcinoma (EHCC) is a refractory malignancy with poor prognosis due to its early invasion, metastasis and recurrence after operation. Therefore, understanding the mechanisms of invasion key development new effective therapeutic strategies for EHCC. In present study we demonstrated that miR-221 promoted EHCC through targeting PTEN formed positive feedback loop β-catenin/c-Jun signaling pathway. We found was upregulated in specimens CC cell lines. Moreover, strongly...
Farnesoid X receptor (FXR) belongs to the nuclear superfamily with its endogenous ligands bile acids. Mice whole body FXR deficiency develop liver tumors spontaneously, but underlying mechanism is unclear. Moreover, it unknown whether in alone serves as a tumor initiator or promoter during carcinogenesis. This study aims evaluate effects of hepatocyte-specific (FXR hep−/− ) formation. The results showed that mice did not show spontaneous tumorigenesis aging (up 24 mo age). Therefore were fed...
Osteosarcoma (OS) is the most common malignant bone tumor found in children. Currently, researchers have focused on protein and gene levels, while associated metabolic variations been poorly understood. In this study, we used a gas chromatography mass spectrometry approach profiled small-molecule metabolites serum urine of 24 OS patients, 19 benign 32 healthy controls, to determine whether there are significant alterations with carcinogenesis. The metabonomic results demonstrate clear...
Farnesoid X receptor (FXR) is a nuclear and key regulator of liver cholesterol triglyceride homeostasis. Scavenger class B type I (SR-BI) critical for reverse transport (RCT) by transporting high-density lipoprotein (HDL) into liver. FXR induces SR-BI, however, the underlying molecular mechanism this induction not known. The current study confirmed SR-BI mRNA activated in mouse livers, human hepatoma cell line, primary hepatocytes. Genome-wide binding analysis livers identified 4 putative...
Colorectal carcinoma (CRC) is the third most commonly diagnosed cancer in world. Phytochemicals have become a research hotspot recent years as prevention and treatment agents due to their low toxicity limited side-effects. Ellagic acid (EA), natural phenolic constituent, displays various biological activities, including anticancer effects. However, detailed mechanisms of EA remain unclear. In present study, we found that inhibited growth HCT-116 colon cells. Moreover, identified...
Inhibitors of IMP dehydrogenase, such as mycophenolic acid (MPA) and mizoribine, which deplete cellular GTP, are used clinically immunosuppressive drugs. The prolonged effect agents on insulin-secreting beta-cells (HIT-T15 INS-1) was investigated. Both MPA mizoribine inhibited mitogenesis, reflected by [3H]thymidine incorporation. Cell number, DNA protein contents, cell (metabolic) viability were decreased about 30%, 60%, 80% after treatment HIT cells with relevant concentrations (e.g. 1...
Background Farnesoid X Receptor (FXR) is a member of the nuclear receptor superfamily and ligand-activated transcription factor essential for maintaining liver intestinal homeostasis. FXR protective against carcinogenesis inflammation in intestine as demonstrated by development tumors knock-out mice. However, mechanisms effects are not completely understood. This study reports novel role regulating expression Sqstm1, which encodes p62 protein. plays an important cellular homeostasis through...
DNA methylation was regarded as the promising biomarker for rectal cancer diagnosis. However, optimal biomarkers with ideal diagnostic performance are still limited. To identify new molecular markers cancer, we mapped and transcriptomic profiles in six paired normal samples. Further analysis revealed hypermethylated probes prone to be located gene promoter. Meanwhile, transcriptome presented 773 low-expressed 1,161 over-expressed genes cancer. Correction identified a panel of 36 an inverse...
Abstract Background Gastric cancer (GC) is the fourth leading cause of cancer‐related death worldwide. Minichromsome maintenance proteins family member 8 (MCM8) assists DNA repair and replication. MCM8 exerts tumor promotor function in multiple digestive system tumors. also considered as a potential therapeutic target. Methods Bioinformatics methods were used to analyze expression clinicopathological significance. was detected by immunohistochemistry (IHC) staining qRT‐PCR. functions GC cell...
Trastuzumab serves as a cornerstone of first-line therapy for HER2-positive (HER2+) breast cancer; however, significant challenge arises due to the emergence resistance within approximately one year commencement treatment, particularly in advanced cases with metastatic disease where its efficacy is limited. Our investigation into tumor tissue from HER2+ cancer patients, employing single-cell sequencing and bioinformatics analysis, has elucidated crucial mechanism underlying reduced...