A5100739943- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Blood groups and transfusion
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Food Quality and Safety Studies
- CAR-T cell therapy research
- Viral-associated cancers and disorders
- Erythrocyte Function and Pathophysiology
- Platelet Disorders and Treatments
- Phytochemicals and Antioxidant Activities
- Multiple Myeloma Research and Treatments
- Hemoglobinopathies and Related Disorders
- Histone Deacetylase Inhibitors Research
- Eosinophilic Disorders and Syndromes
- Kruppel-like factors research
- Protein Degradation and Inhibitors
- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- Blood disorders and treatments
- Metabolism, Diabetes, and Cancer
- Immunodeficiency and Autoimmune Disorders
Samsung Medical Center
2016-2025
Sungkyunkwan University
2016-2025
Kangbuk Samsung Hospital
2016-2022
Soonchunhyang University Hospital
2022
National Cancer Center
2022
Seoul National University Bundang Hospital
2022
International Society for Experimental Hematology
2022
Seoul National University Hospital
2020-2021
Gyeongsang National University Hospital
2017-2019
Gyeongsang National University
2012-2017
Abstract Natural killer (NK) cells are crucial in the innate immune response and show promise cancer immunotherapy, but face challenges activation targeted gene delivery. In this study, bifunctional lipid nanoparticles (DLNPs) containing 1,2‐dioleoyl‐3‐trimethylammonium‐propane (DOTAP), designed to bolster antitumor efficacy of chimeric antigen receptor‐modified NK (CAR‐NK) by facilitating efficient CAR mRNA delivery introduced. The DLNPs, created functionalizing FDA‐approved LNP...
This controlled, split-mouth, preclinical study was designed to evaluate outcome following surgical implantation of an allogeneic, freeze-dried demineralized bone matrix-calcium sulfate (DBM+CS) composite with a CS barrier in 3-wall intrabony periodontal defects 4 dogs. Control conditions included DBM or and gingival flap surgery (GFS) alone. Three-wall (4x4x4 mm) were surgically created at the mesial distal aspect maxillary mandibular first third premolars, respectively. Maxillary each...
추출방법에 따른 몇 가지 약초의 항산화 활성 변화를 연구하였다. 총 5가지 약초(우슬, 목통, 두충, 시호, 향부자)에 대해 비가열 초음파추출, 환류추출 및 열처리 초음파추출을 실시하였다. 폴리페놀 함량은 환류추출과 초음파추출이 초음파추출보다 높았으며, 두충과 향부자를 제외한 세 시료가 환류추출보다 높았다. 플라보노이드 네 초음파추출과 환류추출이 더 많았으며, 우슬과 목통을 시료에서 많았다. ABTS 라디칼 소거능은 작았으며, DPPH 세시료는 환류추출에서 높은 활성을 나타내었다. 환원력은 우슬이 가열처리 시 많은 증가를 나타내었으며, 다른 시료는 차이가 작았다. 본 실험 결과에 따르면, 종류에 따라 적합한 추출방법과 전처리를 적용함으로써 가진 추출물을 얻는 것이 가능할 것이라 판단된다.
Thiopurine S-methyltransferase (TPMT) is a cytoplasmic enzyme involved in the metabolism of thiopurine drugs and its activity largely influenced by polymorphisms TPMT gene. To date, more than 35 variants are known to be associated with reduced activity, but most studies on genotype have included only common nonfunctional variants, such as TPMT*2 TPMT*3. In this study, we carried out complete sequencing analysis screen all Korean patients. A total 900 patients were genotyped for 30 (3.3%) had...
Abstract Objectives Despite its utility, interpreting multiparameter flow cytometry (MFC) data for hematologic malignancy remains time-intensive and complex. This study evaluated the applicability of two dimensionality reduction (DR) algorithms, t-distributed stochastic neighbor embedding (t-SNE) uniform manifold approximation projection (UMAP), to MFC malignancy. Methods A total 237 samples were re-analyzed by t-SNE- UMAP-based gating: 80 with acute leukemia orientation tube panel, 42...
Abstract Background Cancer cachexia is a complex metabolic syndrome that severely impacts patient mobility, treatment strategies, and quality of life. However, no treatments are available to mitigate the debilitating consequences cancer cachexia. Unacylated ghrelin (UnAG), main circulating form ghrelin, enhances muscle growth mitochondrial function in various diseases, but its effects remain be tested. Methods Male C57Bl6/N mice were assigned one three groups: non-tumor-bearing (NTB),...
Background: Although adenoma detection rate (ADR) has been proposed as a quality indicator of colonoscopies, adenomas per colonoscopy (APC) is promising alternative to ADR, it reflects inspection over the entire length colon. This study investigated correlation between ADR and APC, compared efficacy APC based on each with advanced (AADR).Study: Two prospectively collected databases, including 1142 subjects who underwent screening colonoscopies by 28 colonoscopists, were retrospectively...
Measurable residual disease (MRD) is critical in guiding therapeutic strategies for B-cell acute lymphoblastic leukemia (B-ALL). This study evaluated the performance of a novel next-generation sequencing-based Celemics IGH assay (CM-IGH; Celemics, Seoul, Korea) compared with LymphoTrack® FR1 (LT-IGH; Invivoscribe Technologies, USA) and multiparameter flow cytometry (MFC). A total 31 diagnostic 60 follow-up bone marrow aspirate samples, all from same pediatric patients B-ALL, were analyzed...
Potassium-competitive acid blockers (P-CABs) can be used to eradicate Helicobacter pylori infection. We aimed evaluate the impact of treatment duration (7 vs 14 days) on successful H. eradication with P-CAB-based triple therapy in Korea, where clarithromycin resistance rate is high.We retrospectively reviewed data patients who received first-line for infection tegoprazan-based (50 mg tegoprazan + 1000 amoxicillin 500 twice daily 1 or 2 weeks). The primary endpoint was intention-to-treat...
Background Epstein–Barr virus (EBV) quantitation and current imaging modalities are used for diagnosis disease monitoring in Extranodal NK/T cell lymphoma (ENKTL) but have limitations. Thus, we explored the utility of circulating tumor DNA (ctDNA) as a diagnostic biomarker. Methods Through in-depth sequencing 118 blood samples collected longitudinally at different time points from 45 patients, examined mutational profile each sample, estimated its impact on clinical outcome, assessed role...
The objective of this survey was to gain a real-world perspective on coagulation testing by evaluating the availability various laboratory tests, assessing specific analytic and postanalytic steps in clinical laboratories Korea.Participants were surveyed using 65-question questionnaire specifically focused their practices related prothrombin time (PT), activated partial thromboplastin (aPTT), plasma-mixing studies, lupus anticoagulant (LA) platelet function factor assays, composition...
Anti-CD19 chimeric antigen receptor (CAR)-engineered T and NK cell therapies have revolutionized the treatment of B malignancies, but challenges including CD19 loss greatly hinder their full therapeutic potential. Here we demonstrated that co-treatment with anti-CD19 monoclonal antibody enhances antitumor activity CAR-T -NK cells. Even though treated interferes binding CAR, it significantly induces rapid detachment CAR effector cells from target cells, facilitating improved serial killing....
Glutathione reductase (GR) is an enzyme that recycles a key cellular antioxidant molecule glutathione (GSH) from its oxidized form (GSSG) thus maintaining redox homeostasis. A recombinant plasmid to overexpress GR of Brassica rapa subsp. pekinensis (BrGR) in E. coli BL21 (DE3) was constructed using expression vector pKM260. Expression the introduced gene confirmed by semiquantitative RT-PCR, immunoblotting and assays. Purification BrGR protein performed IMAC method indicated dimmer. The...
숙성온도와 기간에 따른 대추의 성분변화 및 항산화활성 변화를 분석한 결과 갈변도는 무처리구에 비해 가온숙성처리구에서 전체적으로 증가하였으며, 숙성 30일차 50일차에서는 다소 감소하는 경향을 보였다. 환원당, fructose와 glucose함량은 높았으며, 숙성온도 기간이 증가함에 따라 유의적으로 감소하였다. 5-HMF 함량은 증가하여 50일차 80℃에서 최대값을 나타내었다. 총 폴리페놀 함량도 DPPH 라디칼소거능 ABTS 라디칼 소거능도 환원력은 숙성온도가 따라서 증가하였지만 숙성일수에 변화는 작게 나타났다. 온도 페놀성 화합물의 증가로 인하여 항산화 활성도 항산화활성을 높일 수 있는 최적숙성조건은 70℃에서 15~30일로 판단되었다. 이를 통하여 건대추의 활성을 향상시켜 새로운 건강기능성 식품소재로 이용가능하며, 이에 활용도 제고 부가가치를 있을 것으로 판단된다.
Chronic granulomatous disease (CGD) is a rare genetic disease, which caused by defects in the NADPH oxidase complex (gp91 phox , p22 p40 p47 and p67 ) of phagocytes.This defect results impaired production superoxide anions other reactive oxygen species (ROS), are necessary for killing bacterial fungal microorganisms leads to recurrent, life-threatening infections inflammation.The dihydrorhodamine (DHR) flow cytometry assay useful diagnostic tool CGD that can detect absent or reduced activity...
Purpose Plasma circulating tumor DNA (ctDNA) could reflect the genetic alterations present in tissues. However, there is little information about clinical relevance of cell-free genotyping peripheral T-cell lymphoma (PTCL).Materials and Methods After targeted sequencing plasma patients with various subtypes PTCL (n=94), we analyzed mutation profiles ctDNA samples their predictive value dynamic monitoring for treatment outcomes.Results mutations were detected 53 (56%, 53/94), detection rate...
Sheath rot symptoms in rice (Oryza sativa) were commonlyobserved during growing season southern part of Korea in2009 and 2010. Symptoms on glumes stems ranged fromnecrotic spot to brown discoloration. Lesion appearance was notuniform, the margins often diffused with an indistinctchlorosis. No water-socking symptom observed (Fig. 1A). Weisolated bacteria from small pieces (3−5 mm) infected tissue ofstem or glume. Tissues surface-sterilized for 30 s 1%hypochlorite solution followed by rinsing...