A5089911968- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Infective Endocarditis Diagnosis and Management
- Enzyme function and inhibition
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Biochemical and Structural Characterization
- Clostridium difficile and Clostridium perfringens research
- Ion channel regulation and function
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Natural product bioactivities and synthesis
- Amino Acid Enzymes and Metabolism
- Microbial Natural Products and Biosynthesis
- Heat shock proteins research
- Antimicrobial Peptides and Activities
- Biotin and Related Studies
- Medicinal Plant Research
- RNA and protein synthesis mechanisms
- Essential Oils and Antimicrobial Activity
University of Tübingen
2019-2024
UCLA Medical Center
2024
Harbor–UCLA Medical Center
2024
Universiti Tunku Abdul Rahman
2014
The present study aims to investigate the analgesic activity of methanol extract galls Quercus infectoria in rats using hot plate and tail‐flick methods. was administered intraperitoneally at a dose 20 mg/kg while morphine sulfate sodium salicylate (10 mg/kg) served as standards. exhibited significant model ( P < 0.05) by increasing reaction time 8.0 sec 30 min after treatment comparison control (4.4 sec). Morphine produced 11.9 same test. At peak (30 min), maximum possible analgesia...
Staphylococcus aureus is a facultative intracellular pathogen. Recently, it has been shown that the protein part of lipoprotein-like lipoproteins (Lpls), encoded by lpl cluster comprising 10 lpls paralogue genes, increases pathogenicity, delays G2/M phase transition, and also triggers host cell invasion. Here, we show recombinant Lpl1 without lipid moiety binds directly to isoforms human heat shock proteins Hsp90α Hsp90ß. Synthetic peptides covering sequence caused twofold fivefold increase...
Rhodomyrtone (Rom) is a plant-derived broad-spectrum antibiotic active against many Gram-positive pathogens. A single point mutation in the regulatory farR gene (farR*) confers resistance to Rom Staphylococcus aureus (RomR). The farR* alters activity of regulator, FarR*, such way that not only its own gene, farR*, but also divergently transcribed farE and genes controlled by global agr, are highly upregulated. Here, we show mainly upregulation fatty acid efflux pump FarE causes RomR...
Abstract The mechanisms behind carbon dioxide (CO 2 ) dependency in non-autotrophic bacterial isolates are unclear. Here we show that the Staphylococcus aureus mpsAB operon, known to play a role membrane potential generation, is crucial for growth at atmospheric CO levels. genes can complement an Escherichia coli carbonic anhydrase (CA) mutant, and CA from E. S. delta- mpsABC mutant. In comparison with wild type, mps mutants produce less hemolytic toxin virulent animal models of infection....
The skin colonizing coagulase-negative Staphylococcus epidermidis causes nosocomial infections and is an important opportunistic highly adaptable pathogen. To gain more insight into this species, we sequenced the genome of biofilm positive, methicillin susceptible S. O47 strain (hereafter O47). This belongs to most frequently isolated sequence type 2. In comparison RP62A strain, can be transformed, which makes it a preferred for molecular studies. O47's has single chromosome about 2.5...
ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) strains are a major challenge for clinicians due, in part, to their resistance most β-lactams, the first-line treatment methicillin-susceptible S. . A phenotype termed “NaHCO 3 -responsiveness” has been identified, wherein many clinical MRSA isolates rendered susceptible standard-of-care β-lactams presence of physiologically relevant concentrations NaHCO , vitro and ex vivo ; moreover, such -responsive” can be effectively cleared by...
CO 2 and bicarbonate are required for carboxylation reactions in central metabolism biosynthesis of small molecules all bacteria. This is achieved by two different systems dissolved inorganic carbon supply (DICS): these the membrane potential-generating system (MpsAB) carbonic anhydrase (CA), but both rarely coexist a given species.
MRSA infections are increasingly difficult to treat, due in part their resistance most β-lactam antibiotics. A novel and relatively common phenotype, termed NaHCO 3 responsiveness, has been identified which strains show increased susceptibility vitro vivo β-lactams the presence of .
ABSTRACT NaHCO 3 responsiveness is a novel phenotype where some methicillin-resistant Staphylococcus aureus (MRSA) isolates exhibit significantly lower minimal inhibitory concentrations (MIC) to oxacillin and/or cefazolin in the presence of . correlated with treatment response β-lactams an endocarditis animal model. We investigated whether -responsive strains was associated faster clearance bacteremia. The CAMERA2 trial (Combination Antibiotics for Methicillin-Resistant ) randomly assigned...
Bicarbonate and CO2 are essential substrates for carboxylation reactions in bacterial central metabolism. In Staphylococcus aureus, the bicarbonate transporter, MpsABC (membrane potential-generating system) is only carbon concentrating system. A mpsABC deletion mutant can hardly grow ambient air. this study, we investigated changes that occur S. aureus when it suffers from CO2/bicarbonate deficiency. Electron microscopy revealed has a twofold thicker cell wall thickness compared to parent...
In recent years, it became apparent that not only autotrophic but also most other bacteria require CO2 or bicarbonate for growth. Two systems are available the acquisition of dissolved inorganic carbon supply (DICS): cytoplasmic localized carbonic anhydrase (CA) and more recently described transporter MpsAB (membrane potential generating system). pathogenic species Staphylococcus aureus, there contradictions in literature regarding presence a CA MpsAB. Here, we address these detail. We could...
Bicarbonate and CO