A5066403075- Effects and risks of endocrine disrupting chemicals
- Pregnancy and Medication Impact
- Reproductive Biology and Fertility
- Birth, Development, and Health
- Sperm and Testicular Function
- Congenital heart defects research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Pancreatic function and diabetes
- Estrogen and related hormone effects
- Sexual Differentiation and Disorders
- Pharmaceutical studies and practices
- Testicular diseases and treatments
- Reproductive System and Pregnancy
- Carcinogens and Genotoxicity Assessment
- Single-cell and spatial transcriptomics
- Ovarian function and disorders
- Cannabis and Cannabinoid Research
- Pharmacological Effects and Toxicity Studies
- Animal Genetics and Reproduction
- Genetics and Neurodevelopmental Disorders
- Urological Disorders and Treatments
- RNA modifications and cancer
- Hedgehog Signaling Pathway Studies
- CRISPR and Genetic Engineering
Institut de Recherche en Santé, Environnement et Travail
2016-2025
École des Hautes Études en Santé Publique
2018-2025
Inserm
2016-2025
Université de Rennes
2015-2024
MRC Centre for Reproductive Health
2018
The Queen's Medical Research Institute
2018
University of Edinburgh
2018
Centre Hospitalier Universitaire de Rennes
2018
University of Copenhagen
2018
Novo Nordisk (Denmark)
2018
Tissue integrity relies on barriers formed between epithelial cells. In the testis, barrier is at initiation of puberty by a tight junction complex adjacent Sertoli cells, thereby defining an adluminal compartment where meiosis and spermiogenesis occur. Claudin 11 obligatory protein for formation in testis. It expressed spermatogenesis does not proceed beyond its absence, resulting male sterility. cell maturation—arrest proliferation expression proteins to support germ development—parallels...
Masculinization depends on the fetal testis. Exposure of human fetus during pregnancy to paracetamol and/or other mild analgesics is associated with an increased risk cryptorchidism. We aimed determine whether disrupted morphology and endocrine function used in vitro system based culture testes exposed or not paracetamol, its metabolite N-(4-hydroxyphenyl)-arachidonoylethanolamide (AM404), aspirin, indomethacin, ketoconazole at 10−4 10−7 M. The study was conducted University Rennes I. Human...
Concern has been raised over increased male reproductive disorders in the Western world, and disruption of endocrinology suggested to play a central role. Several studies have shown that mild analgesics exposure during fetal life is associated with antiandrogenic effects congenital malformations, but on adult man remain largely unknown. Through clinical trial young men exposed ibuprofen, we show analgesic resulted condition named "compensated hypogonadism," prevalent among elderly physical...
Forty first-trimester human hearts were studied to lay groundwork for further studies of principles underlying congenital heart defects. We first sampled 49,227 cardiac nuclei from three fetuses at 8.6, 9.0, and 10.7 post-conceptional weeks (pcw) single-nucleus RNA sequencing, enabling distinction six classes comprising 21 cell types. Improved resolution led identification novel cardiomyocytes minority autonomic lymphatic endothelial transcriptomes, among others. After integration with 5-7...
In mammals, anti-Müllerian hormone (AMH) is produced by Sertoli cells from the onset of testicular differentiation and granulosa after birth. birds, AMH starts to be expressed in indifferent gonads both sexes at a similar level later up-regulated males. We previously demonstrated that, unlike expression occurs chick embryo absence SOX9. looked for potential factors that might involved regulating different stages gonad comparing its pattern embryos young chicken with DMRT1, SF-1, WT1, GATA-4,...
Numerous chemicals are capable of disrupting androgen production, but the possibility that they might act together to produce effects greater than those most effective component in mixture has not been studied directly human tissues. Suppression synthesis fetal life associated with testis maldescent, malformations genitalia at birth, and poor semen quality later life.Our aim was investigate whether can disrupt production explants evaluate importance when characterizing hazard individual...
Are bisphenol A (BPA) and BPA analogs (BPA-A) safe for male human reproductive function?The endocrine function of testes explants [assessed by measuring testosterone insulin-like factor 3 (INSL3)] was impacted exposure the adult testis to BPA/BPA-A.The few epidemiologic studies performed suggest that bisphenols have potential disruptive properties, but they did not identify clear direct patterns disruption.Adult in culture were exposed F (BPF), S (BPS), E (BPE), B (BPB) diglycidyl ether...
Studies report that fetal exposure to paracetamol/acetaminophen by maternal consumption can interfere with male reproductive development. Moreover, recent biomonitoring data widespread presence of paracetamol in German and Danish populations, suggesting via secondary (nonpharmaceutical) sources, such as metabolic conversion from the ubiquitous industrial compound aniline. In this study, we investigated extent which aniline female Intrauterine gavage pregnant dams resulted shortening...
The Hedgehog (Hh) family of secreted proteins act as morphogens to control embryonic patterning and development in a variety organ systems. Post-translational covalent attachment cholesterol palmitate Hh are critical for multimerization long range signaling potency. However, the biological impact lipid modifications on ligand distribution signal reception humans remains unclear. In present study, we report unique case autosomal recessive syndromic 46,XY Disorder Sex Development (DSD) with...
Abstract Among pregnant women ibuprofen is one of the most frequently used pharmaceutical compounds with up to 28% reporting use. Regardless this, it remains unknown whether could act as an endocrine disruptor reported for fellow analgesics paracetamol and aspirin. To investigate we exposed human fetal testes (7–17 gestational weeks (GW)) using ex vivo culture xenograft systems. Ibuprofen suppressed testosterone Leydig cell hormone INSL3 during 8–9 GW concomitant reduction in expression...
Few studies have been undertaken to assess the possible effects of bisphenol A (BPA) on reproductive hormone balance in animals or humans with often contradictory results. We investigated direct endocrine disruption by BPA fetal testes 2 rat strains (14.5-17.5 days post-coitum) and (8-12 gestational weeks) under different culture conditions. concentrations 10(-8)M 10(-5)M for 72 h reduced testosterone production Sprague-Dawley testes, while only 10-5M suppressed it Wistar strain. The...
Industrial use of aniline is increasing worldwide with production estimated to surpass 5.6 million metric tons in 2016. Exposure occurs via air, diet, and water augmenting the risk exposing a large number individuals. Early observations suggest that metabolized paracetamol/acetaminophen, likely explaining omnipresence low concentrations paracetamol European populations. This concern as recent studies implicate disrupter reproduction. Here, we show through steroidogenic profiling exposure led...
Abstract Although it has been suggested that in mammals the loss of female germ cells may induce masculinization ovarian compartment, there as yet no conclusive demonstration. To directly address question, present study designed to determine fate follicular after oocyte loss. Using γ-irradiation selectively deplete oocytes nongrowing follicles rats, we show oocyte-depleted survive, proliferate, and subsequently acquire morphological characteristics Sertoli cells: elongated cytoplasm, basal...
The widely-used herbicide atrazine (ATZ) is detected in ground and surface water many countries. Several studies animals have demonstrated that ATZ has endocrine-disrupting effects on male female reproduction vertebrate species. In this study, we investigated the of exposure meiosis, a key step gametogenesis mammals. treatment was initiated before oocyte entry into which occurs during embryonic period females. We found to increases level 8-oxo-guanine nucleus meiotic cells, reflecting...
The evolution and development of the head have long captivated researchers due to crucial role as gateway for sensory stimuli intricate structural complexity head. Although significant progress has been made in understanding various vertebrate species, our knowledge early human ontogeny remains limited. Here, we used advanced whole-mount immunostaining 3D imaging techniques generate a comprehensive cellular atlas embryogenesis. We present detailed developmental series diverse tissues cell...
Abstract Aims/hypothesis Rodent pancreas development has been described in great detail. On the other hand, there are still gaps our understanding of developmental trajectories pancreatic cells during human ontogenesis. Here, aim was to map spatial and chronological dynamics cell differentiation proliferation by using 3D imaging cleared embryonic fetal pancreases. Methods We combined tissue clearing with light-sheet fluorescence pancreases first trimester pregnancy. In addition, we validated...
Does ibuprofen use during the first trimester of pregnancy interfere with development human fetal ovary?In fetuses, exposure is deleterious for ovarian germ cells.In utero stages define future reproductive capacity a woman. In rodents, analgesics can impair ovary leading to early onset fertility failure. Ibuprofen, which available over-the-counter, has been reported as frequently consumed medication pregnancy, especially when cells undergo crucial steps proliferation and...