A5090084983- Immune cells in cancer
- Galectins and Cancer Biology
- Cancer Immunotherapy and Biomarkers
- Protein Tyrosine Phosphatases
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Atherosclerosis and Cardiovascular Diseases
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- Macrophage Migration Inhibitory Factor
- Cancer Research and Treatments
- Urinary Bladder and Prostate Research
- Fibroblast Growth Factor Research
- Histone Deacetylase Inhibitors Research
- Steroid Chemistry and Biochemistry
- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Cholesterol and Lipid Metabolism
- Nanoplatforms for cancer theranostics
- Prostate Cancer Treatment and Research
- Prostate Cancer Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Pneumonia and Respiratory Infections
- Hippo pathway signaling and YAP/TAZ
- interferon and immune responses
Purdue University West Lafayette
2013-2025
George Washington University
2023-2024
Purdue University Institute for Cancer Research
2023
Center for Cancer Research
2019-2022
Royal Derby Hospital
2019
Southern Illinois University Carbondale
2010
Abstract Autoimmune (AI) diseases can affect many organs; however, the prostate has not been considered to be a primary target of these systemic inflammatory processes. Here, we utilize medical record data, patient samples, and in vivo models evaluate impact inflammation, as seen AI diseases, on tissue. Human mouse tissues are used examine whether targeting inflammation limits prostatic hyperplasia. Evaluation 112,152 records indicates that benign hyperplasia (BPH) prevalence is...
Macrophages exhibit marked phenotypic heterogeneity within and across disease states, with lipid metabolic reprogramming contributing to macrophage activation heterogeneity. Chronic inflammation has been observed in human benign prostatic hyperplasia (BPH) tissues, however states their contributions this hyperplastic have not defined. We postulated that a shift phenotypes increasing prostate size could involve alterations resulting epithelial or stromal hyperplasia. Single-cell RNA-seq of...
A group of chemotherapeutic drugs has gained increasing interest in cancer immunotherapy due to the potential induce immunogenic cell death (ICD). critical challenge using ICD inducers is immunotoxicity accompanying their antiproliferative effects. To alleviate this, a nanocapsule formulation carfilzomib (CFZ), an ICD-inducing proteasome inhibitor, was developed interfacial supramolecular assembly tannic acid (TA) and iron, supplemented with albumin coating. The albumin-coated CFZ...
Abstract Although immunotherapies of tumors have demonstrated promise for altering the progression malignancies, been limited by an immunosuppressive tumor microenvironment (TME) that prevents infiltrating immune cells from performing their anticancer functions. Prominent among are myeloid-derived suppressor (MDSC) and tumor-associated macrophages (TAM) inhibit T via release cytokines engagement checkpoint receptors. Here, we explore properties MDSCs TAMs freshly isolated mouse human find...
Significance The rationale of local cancer immunotherapy is that the treated tumor cells can serve as a depot antigens and activate/mobilize patient’s immune system to address systemic diseases. However, challenge coordinate several events involved in activation antitumor responses, colocalize retain multiple therapies tumors, support functions cells. Our carrier polyethyleneimine-lithocholic acid conjugate (2E′) addresses these challenges based on amphiphilic structure inherent...
Pro-inflammatory macrophages play a prominent role in such autoimmune diseases as rheumatoid arthritis, Crohn's disease, psoriasis, sarcoidosis, and atherosclerosis. Because pro-inflammatory have also been shown to overexpress receptor for the vitamin folic acid (i.e., folate beta; FR-β), folate-linked drugs explored use imaging treatment of these same diseases. To determine whether allergic inflammatory disorders might be similarly targeted with drugs, we examined characteristics that are...
Essentials Elimination of PDAC tumor cell PAR1 increased cytotoxic T cells and reduced macrophages. PAR1KO are preferentially eliminated from growing tumors. Thrombin-PAR1 signaling in drives an immunosuppressive gene signature. Csf2 Ptgs2 thrombin-PAR1 downstream immune suppressor genes cells. ABSTRACT: Background Pancreatic ductal adenocarcinoma (PDAC) is characterized by a prothrombotic state lack host antitumor responsiveness. Linking these two key features, we previously demonstrated...
Abstract The effectiveness of immunotherapy as a treatment for metastatic breast cancer is limited due to low numbers infiltrating lymphocytes in lesions. Herein, we demonstrated that adjuvant therapy using FIIN4, covalent inhibitor fibroblast growth factor receptor (FGFR), dramatically delayed the pulmonary metastases syngeneic models cancer. In addition, model systemic tumor dormancy targeting FGFR enhanced immunogenicity microenvironment through increased infiltration CD8+ and reduced...
Folate receptors can perform folate transport, cell adhesion, and/or transcription factor functions. The beta isoform of the receptor (FRβ) has attracted considerable attention as a biomarker for immunosuppressive macrophages and myeloid-derived suppressor cells, however, its role in immunosuppression remains uncharacterized. We demonstrate here that FRβ cannot bind on healthy tissue macrophages, but does after macrophage incubation anti-inflammatory cytokines or cancer cell-conditioned...
Macrophages exhibit marked phenotypic heterogeneity within and across disease states, with lipid metabolic reprogramming contributing to macrophage activation heterogeneity. Chronic inflammation has been observed in human benign prostatic hyperplasia (BPH) tissues, however states their contributions this hyperplastic have not defined. We postulated that a shift phenotypes increasing prostate size could involve alterations resulting epithelial or stromal hyperplasia. Single-cell RNA-seq of...
Evidence linking prostatitis and prostate cancer development is contradictory. To study this link, the POET3 mouse, an inducible model of prostatitis, was crossed with a Pten-loss (Pten+/−) containing ROSA26 luciferase allele to monitor size. Prostatitis induced, bioluminescence tracked over 12 months, lesion development, inflammation, cytokine expression analyzed at 4, 8, months compared mice without induction prostatitis. Acute led more proliferative epithelium enhanced bioluminescence....
Abstract Benign prostatic hyperplasia (BPH) is among the most common age-associated diseases in men; however, contribution of age-related changes immune cells to BPH not clear. The current study determined that an CD8 + T cell subset (Taa) with high Granzyme K ( GZMK hi ) and low B GZMB gene expression infiltrate aged human prostates positively correlate International Prostate Symptom Score (IPSS). A velocity analysis indicated differentiation altered large compared small age-matched...
Chronic prostate inflammation in patients with benign hyperplasia (BPH) correlates the severity of symptoms. How contributes to enlargement and/or BPH symptoms and underlying mechanisms remain unclear. In this study, we utilize a unique transgenic mouse model that mimics chronic non-bacterial prostatitis men investigate impact on androgen receptor (AR) basal stem cells (bPSC) their differentiation vivo. We find significantly enhances AR levels activity bPSC. More importantly, identify...
Warm, fresh whole blood (WB) has been used by the US military to treat casualties in Iraq and Afghanistan. Based on data that setting, cold-stored WB hemorrhagic shock severe bleeding civilian trauma patients United States. In an exploratory study, we performed serial measurements of WB's composition platelet function during cold storage. Our hypothesis was vitro adhesion aggregation would decrease over time.WB samples were analyzed storage days 5, 12, 19. Hemoglobin, count, gas parameters...
Cholesterol sulfotransferase, SULT2B1b, has been demonstrated to modulate both androgen receptor activity and cell growth properties. However, the mechanism(s) by which SULT2B1b alters these properties within prostate cancer cells not described. Furthermore, specific advantages of expression in are understood. In studies, single-cell mRNA sequencing was conducted compare transcriptomes knockdown (KD) versus Control KD LNCaP cells. Over 2,000 differentially expressed genes were identified...
SH2 containing protein tyrosine phosphatase-2 (SHP2) is recognized as a druggable oncogenic phosphatase that expressed in both tumor cells and immune cells. How cell-autonomous SHP2 contributes to an immunosuppressive microenvironment (TME) therapeutic failure of checkpoint blockades metastatic breast cancer (MBC) not fully understood. Herein, we utilized systemic inhibition inducible genetic depletion investigate reprogramming during targeting. Pharmacologic sensitized MBC growing the lung...
Abstract Benign prostatic hyperplasia (BPH) is ostensibly linked to autoimmune (AI) diseases, but whether the prostate a target of systemic inflammation associated with AI conditions unknown. Prostatic fibrosis, hyperplasia, and reduced responses BPH-related medical therapies. This study was conducted determine if disease correlates BPH diagnosis targeting an inflammatory mediator limits hyperplasia. Patient records (n=112,152) were evaluated prevalence among different diseases. Inflammatory...
The embryonic environment can modify cancer cell metabolism, and it is reported to induce the loss of tumorigenic properties even affect differentiation cells into normal tissues. cellular mechanisms related this remarkable phenomenon, which likely mediated by cell-to-cell communication, have been previously investigated with particular focus on proteins genes involved. In study we report optimization results a straightforward in vitro system where mouse prostate carcinoma (RM-1) were...
<div>Abstract<p>Although immunotherapies of tumors have demonstrated promise for altering the progression malignancies, been limited by an immunosuppressive tumor microenvironment (TME) that prevents infiltrating immune cells from performing their anticancer functions. Prominent among are myeloid-derived suppressor (MDSC) and tumor-associated macrophages (TAM) inhibit T via release cytokines engagement checkpoint receptors. Here, we explore properties MDSCs TAMs freshly isolated...