A5084993019- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Cancer, Stress, Anesthesia, and Immune Response
- Ovarian cancer diagnosis and treatment
- Histone Deacetylase Inhibitors Research
- Inflammatory Biomarkers in Disease Prognosis
- Prostate Cancer Treatment and Research
- BRCA gene mutations in cancer
- Medicinal Plant Research
- Beetle Biology and Toxicology Studies
- Testicular diseases and treatments
- Ferroptosis and cancer prognosis
- Mesenchymal stem cell research
- Sarcoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Advanced Breast Cancer Therapies
- Chemokine receptors and signaling
- Bioactive Compounds and Antitumor Agents
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Sesame and Sesamin Research
- Ion channel regulation and function
- Lung Cancer Research Studies
Biomedical Research Center of the Slovak Academy of Sciences
2016-2024
Cancer Research Institute of the Slovak Academy of Sciences
2016-2024
Slovak Academy of Sciences
2019-2021
Breast cancer comprises a substantial proportion of diagnoses in women and is primary cause cancer-related mortality. While hormone-responsive cases generally have favorable prognosis, the aggressive nature triple-negative breast presents challenges, with intrinsic resistance to established treatments being persistent issue. The complexity intensifies emergence acquired resistance, further complicating management cancer. Epigenetic changes, encompassing DNA methylation, histone RNA...
Acquired drug resistance and metastasis in breast cancer (BC) are coupled with epigenetic deregulation of gene expression. Epigenetic drugs, aiming to reverse these aberrant transcriptional patterns sensitize cells other therapies, provide a new treatment strategy for drug-resistant tumors. Here we investigated the ability DNA methyltransferase (DNMT) inhibitor decitabine (DAC) increase sensitivity BC anthracycline antibiotic doxorubicin (DOX). Three cell lines representing different...
Cisplatin resistance in testicular germ cell tumors (TGCTs) is a clinical challenge. We investigated the underlying mechanisms associated with cancer stem (CSC) markers and modalities circumventing chemoresistance. Chemoresistant models (designated as CisR) of human embryonal carcinoma lines NTERA-2 NCCIT were derived characterized using flow cytometry, gene expression, functional protein arrays. Tumorigenicity was determined on immunodeficient mouse model. Disulfiram used to examine...
During cancer progression, breast tumor cells interact with adjacent adipose tissue, which has been shown to be engaged in aggressiveness. However, the tumor-directed changes tissue-resident stromal affected by tumor-stroma communication are still poorly understood. The acquired might remain tissue even after removal and may contribute relapse. We investigated functional properties (migratory capacity, expression secretion profile) of mesenchymal isolated from healthy (n = 9) tumor-distant...
A correlation between circulating tumor cells (CTCs) and monocytes in metastatic breast cancer (BC), where CTCs monocyte-to-lymphocyte ratio (MLR) were predictors of overall survival (OS), was recently shown. Herein, we aimed to assess the association complete blood count (CBC)-derived inflammation-based scores 284 primary BC patients. determined CD45-depleted peripheral mononuclear by real time-PCR. This method allowed us detect a subset with an epithelial-to-mesenchymal transition...
Aims: Chemotherapy resistance remains a major challenge in breast cancer (BC) treatment. This study investigated whether development is associated with DNA methylation changes and assessed the potential of methyltransferase inhibitor decitabine (DAC) to reverse these alterations enhance chemosensitivity. Methods: Molecular profiling functional assays were used characterize paclitaxel- (PAC) doxorubicin- (DOX) resistant BC cell lines derived from luminal A (T-47D), triple-negative...
Cisplatin resistance of ovarian yolk sac tumors (oYST) is a clinical challenge due to dismal patient prognosis, even though the disease extremely rare. We investigated potential association between cisplatin and cancer stem cell (CSC) markers in chemoresistant oYST cells targeting strategies overcome oYST.Chemoresistant were derived from chemosensitive human by cultivation vitro. Derivative characterized chemoresistance, functional assays, flow cytometry, gene expression protein arrays...
Chemotherapy remains a standard treatment option for breast cancer despite its toxic effects to normal tissues. However, the long-lasting of chemotherapy on non-malignant cells may influence tumor cell behavior and response treatment. Here, we have analyzed doxorubicin (DOX) paclitaxel (PAC), commonly used chemotherapeutic agents, survival cellular functions mesenchymal stromal (MSC), which comprise an important part microenvironment.Chemotherapy-exposed MSC (DOX-MSC, PAC-MSC) were...
The majority of patients with testicular germ cell tumors (GCTs) can be cured cisplatin-based chemotherapy. However, for a subset present cisplatin-refractory disease, which confers poor prognosis, the treatment options are limited. Novel therapies therefore urgently needed to improve outcomes in this challenging patient population. It has previously been shown that Wnt/β-catenin signaling is active GCTs suggesting its inhibitors LGK974 and PRI-724 may show promise management GCTs. We herein...
A Disintegrin And Metalloprotease 23 (ADAM23), a member of the ADAM family, is involved in neuronal differentiation and cancer. ADAM23 considered possible tumor suppressor gene frequently downregulated various types malignancies. Its epigenetic silencing through promoter hypermethylation was observed breast cancer (BC). In present study, we evaluated prognostic significance methylation for hematogenous spread disease-free survival (DFS). Pyrosequencing used to quantify tumors 203 BC...
Epithelial-to-mesenchymal transition (EMT) significantly affects the risk of metastasising in breast cancer. Plasticity and reversibility EMT suggest that epigenetic mechanisms could be key drivers these processes, but little is known about dynamics EMT-related alterations. We hypothesised EMT, mediated by autocrine paracrine signals, will accompanied changes DNA methylation profiles. Therefore, conditioned medium from adipose tissue-derived mesenchymal stromal cells was used for induction...
Circulating tumor cells (CTCs) and the immune infiltration of tumors are closely related to clinical outcomes. This study aimed verify influence stromal lymphocyte context microenvironment on hematogenous spread prognosis 282 chemotherapy naïve primary BC patients. To detect presence mesenchymal CTCs, RNA extracted from CD45-depleted peripheral blood was interrogated for expression gene transcripts. Tumor-infiltrating lymphocytes (TILs) were detected in areas by immunohistochemistry, using...
Abstract Programmed death ligand 1 (PD-L1) overexpression has been associated with poor clinical outcomes in several human cancers whose increased malignant behaviour might be related to PD-L1 mediated systemic immunological tolerance. This study aims verify if circulating cytokines may serve as a proxy for non-invasive identification of sensitive prognostic biomarkers reflecting tumour and its microenvironment. Immunohistochemistry was used measure expression tissue sections 148 chemonaïve...
Breast cancer metastases are the main reason for women´s highest mortality.Even though tumor cell dissemination via circulating cells (CTC) released from primary site is a very ineffective process, distant appear in 46% of triple-negative breast (TNBC) patients corresponding to disease aggressiveness.Laboratory models functional testing which mimic spread metastatic needed efficient investigation underlying mechanisms and therapeutic intervention.Here, we describe novel isogenic variants...
Introduction Circulating tumour cells (CTCs) are associated with metastatic potential in a variety of human cancers. Although DNA methylation cancer-related genes is promising diagnostic and prognostic tool, it was rarely studied relation to the presence CTCs. ADAM23 gene, member ADAM (a disintegrin metalloproteinase domain) family, involved various biological events such as cell adhesion, fusion, migration, membrane protein shedding proteolysis. The aberrant expression silencing breast,...