A5102997444- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Nutritional Studies and Diet
- DNA and Nucleic Acid Chemistry
- Plant and animal studies
- Chemical Safety and Risk Management
- Advanced Chemical Sensor Technologies
- Cerebral Venous Sinus Thrombosis
- Nanoparticles: synthesis and applications
- Dietary Effects on Health
- Extracellular vesicles in disease
- Intracranial Aneurysms: Treatment and Complications
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Genetically Modified Organisms Research
- Allelopathy and phytotoxic interactions
- Cancer therapeutics and mechanisms
- interferon and immune responses
- Genomics, phytochemicals, and oxidative stress
- Vascular Malformations Diagnosis and Treatment
- Pesticide Exposure and Toxicity
- Vitamin C and Antioxidants Research
Maastricht University
2023-2025
Oslo Cancer Cluster
2023-2025
Guangdong Academy of Medical Sciences
2015-2023
Guangdong Provincial People's Hospital
2020-2023
University of Oslo
2023
Southern Medical University
2023
University of Debrecen
2018
Guangdong General Hospital
2015
Acquired drug resistance and metastasis in breast cancer (BC) are coupled with epigenetic deregulation of gene expression. Epigenetic drugs, aiming to reverse these aberrant transcriptional patterns sensitize cells other therapies, provide a new treatment strategy for drug-resistant tumors. Here we investigated the ability DNA methyltransferase (DNMT) inhibitor decitabine (DAC) increase sensitivity BC anthracycline antibiotic doxorubicin (DOX). Three cell lines representing different...
Measurement of DNA migration in the comet assay can be done by image analysis or visual scoring. The latter accounts for 20%-25% published results. Here we assess intra- and inter-investigator variability scoring comets. We include three training sets images, which used as reference researchers who wish to use Investigators 11 different laboratories scored images using a five-class system. There is variation comets (i.e. coefficient (CV) = 9.7%, 19.8%, 15.2% I-III, respectively). However,...
The comet assay is a simple and versatile method for measurement of DNA damage in eukaryotic cells. More specifically, the detects migration from agarose gel-embedded nucleoids, which depends on conditions level damage. Certain steps procedure have substantial impact magnitude (e.g. electric potential time electrophoresis). Inter-laboratory variation levels occurs because there no agreement optimal or suitable controls. purpose hCOMET ring trial was to test potassium bromate (KBrO3) as...
The formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay is widely used for the measurement of oxidatively generated damage to DNA. However, there has not been a recommended long-term positive control this version assay. We have investigated potassium bromate as Fpg-modified because it generates many Fpg-sensitive sites with little concurrent generation strand breaks. Eight laboratories same procedure treatment monocytic THP-1 cells (0, 0.5, 1.5, and 4.5 mM) subsequent...
A risk assessment on the aquatic toxicity of plant biostimulant strigolactone mimic (2-(4-methyl-5-oxo-2,5-dihydro-furan-2-yloxy)-benzo[de]isoquinoline-1,3-dione (SL-6) was performed using a suite standardised bioassays representing different trophic groups and acute chronic endpoints. In freshwater, three algae, crustacea fish were used. Whilst in seawater, algae (unicellular macroalgae), Crustacea Mollusca employed. addition, genotoxicity SL-6 determined with comet unicellular marine...
DNA damage caused by oxidative reactions plays a crucial role in the pathogenesis of colorectal cancer (CRC). In previous cross-sectional study, CRC patients diagnosed with regional disease (stage III) exhibited higher level base oxidation peripheral blood mononuclear cells (PBMCs) 2-9 months post-surgery compared to those localized I-II). To further explore this observation over time, present study aimed investigate versus 6 and 12 after initial measurements. The included enrolled...
To investigate the expression of miR-130a in human colon cancer patients and its specific mechanism regulating biological function cells.Cancer tissues, paracancerous serum samples 40 who underwent surgery The Second Affiliated Hospital Qiqihar Medical University from May 2018 to March 2019 were collected, healthy volunteers received physical examination collected. Real Time-quantitative Polymerase Chain Reaction (qRT-PCR) was used detect miR-130a. Human cell divided into mimic group,...
Abstract Background Hazard and risk assessment of nanomaterials (NMs) face challenges due to, among others, the numerous existing nanoforms, discordant data conflicting results found in literature, specific application strategies such as grouping read-across, emphasizing need for New Approach Methodologies (NAMs) to support Next Generation Risk Assessment (NGRA). Here these are addressed a study that couples physico-chemical characterization with vitro investigations silico similarity...
DNA repair is an essential agent in cancer development, progression, prognosis, and response to therapy. We have adapted a cellular assay based on the formamidopyrimidine glycosylase (Fpg)-modified comet assess kinetics. The removal of oxidized nucleobases over time (0-480 min) was analyzed peripheral blood mononuclear cells (PBMCs) 8 cell lines. damage induced by exposure either Ro19-8022 plus visible light or potassium bromate (KBrO
Glioblastoma (GBM) is the most common malignant primary brain tumour in adults. VSIG4 has been identified to be associated with GBM. We aimed determine downstream regulatory mechanisms of GBM.Differential expression was analysed using GEPIA. The assessed by RT-qPCR and its genes were screened transcriptome sequencing. pyroptosis-related proteins JAK2/STAT3 pathway measured Western blotting. GBM cell viability, migration, invasion detected CCK-8, scratch, Transwell assays. levels factors...
Abstract The comet assay is widely used in biomonitoring studies for the analysis of DNA damage leukocytes and peripheral blood mononuclear cells. Rather than processing samples directly, it can be desirable to cryopreserve whole or isolated cells later by assay. However, this creates concern about artificial accumulation during cryopreservation. In study, 10 laboratories standardized cryopreservation thawing procedures monocytic (THP-1) lymphocytic (TK6) Samples were cryopreserved small...
DNA repair is essential to maintain genomic integrity and may affect colorectal cancer (CRC) patients' risk of secondary cancers, treatment efficiency, susceptibility various comorbidities. Bioactive compounds identified in plant food have the potential modulate mechanisms, but there limited evidence how dietary factors activity CRC patients remission after surgery. The aim this study was investigate effect a 6 months personalized intensive intervention on post-surgery (stage I-III). present...
Abstract Nucleotide excision repair (NER) is crucial for repairing bulky lesions and crosslinks in DNA caused by exogenous endogenous genotoxins. The number of studies that have considered as a biomarker limited, therefore one the primary objectives European COST Action hCOMET (CA15132) was to assemble analyze pooled database with data on NER activity. comprised 738 individuals, gathered from 5 laboratories ran population using comet-based vitro assay. activity peripheral blood mononuclear...
Few protocols exist to retrieve male germ cell-specific information regarding DNA damage dynamics, or assess potential detrimental effects of environmental contaminants, drugs, lifestyle-factors related genotoxicity. We provide a protocol for measuring testicular germ-cell genotoxicity using revised version the alkaline comet assay in rodent cells. describe experimental design, generation cell suspensions, analyses, germ-cell-specific scoring, and data curation approaches collect two...
DNA repair plays an essential role in maintaining genomic stability, and can be assessed by various comet assay-based approaches, including the cellular assay vitro assay. In assay, cells are challenged with a DNA-damaging compound damage removal over time is assessed. early step process as ability of extract to recognize incise damaged substrate nucleoids from treated compound. Our direct comparison both assays eight cell lines human peripheral blood lymphocytes indicated no significant...
microRNAs (miRs) have been reported as blood-based noninvasive indicators for the diagnosis of various diseases. However, utility whole miRs in intracranial aneurysm (IA) is still not clear. The present study aimed to examine miR expression profiling peripheral blood IA patients and healthy controls.