A5100422129- Ubiquitin and proteasome pathways
- Parkinson's Disease Mechanisms and Treatments
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
- Inflammasome and immune disorders
- Nuclear Receptors and Signaling
- Peptidase Inhibition and Analysis
- Neurological diseases and metabolism
- ATP Synthase and ATPases Research
- Nerve injury and regeneration
- Hepatocellular Carcinoma Treatment and Prognosis
- Kidney Stones and Urolithiasis Treatments
- Sepsis Diagnosis and Treatment
- Amyotrophic Lateral Sclerosis Research
- Genetic Neurodegenerative Diseases
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hereditary Neurological Disorders
- Lysosomal Storage Disorders Research
Shanghai Jiao Tong University
2024-2025
First Affiliated Hospital of Zhengzhou University
2024
Qingdao University
2024
Affiliated Hospital of Qingdao University
2024
Southern University of Science and Technology
2023
The University of Texas MD Anderson Cancer Center
2023
Seventh People's Hospital of Shanghai
2018-2023
Third Xiangya Hospital
2019-2022
Central South University
2018-2022
Soochow University
2021
This study aimed to determine the mutational spectrum of familial Parkinson's disease and sporadic early-onset (sEOPD) in a mainland Chinese population clinical features mutation carriers. We performed multiplex ligation-dependent probe amplification assays whole-exome sequencing for 1676 unrelated patients with population, including 192 probands from families autosomal-recessive disease, 242 autosomal-dominant 1242 sEOPD (age at onset ≤ 50). According standards guidelines American College...
Background: Abnormal activation of microglia occurs in the early stage Alzheimer's disease (AD) and leads to subsequent neuroinflammation major AD pathologies. Circular RNAs (circRNAs) are emerging as great potential therapeutic targets AD. However, extent circRNAs entwined underlying mechanism microglia-driven remain elusive. Methods: The circular RNA Dlg1 (circDlg1) was identified using circRNA microarray screening magnetic-isolated APP/PS1 mice. CircDlg1 expression mice patients validated...
Glioblastoma multiforme (GBM) is one of the most malignant primary tumors in humans. Despite standard therapeutic strategy with tumor resection combined radiochemotherapy, prognosis remains disappointed. Recently, deubiquitinating enzymes (DUBs) has been reported as potential cancer therapy targets due to their multifunctions involved regulation tumorigenesis, cell cycle, apoptosis, and autophagy. In this study, we found that knockdown ubiquitin specific protease (USP5), a family member DUB,...
Parkinson’s disease (PD) is a complex neurodegenerative disorder with strong genetic component. A growing number of variants and genes have been reported to be associated PD; however, there no database that integrate different type data, support analyzing PD-associated (PAGs). By systematic review curation multiple lines public studies, we layers data (rare copy-number identified from patients PD, genome-wide association differentially expressed genes, differential DNA methylation genes) age...
CDK4 is crucial for G1-to-S transition of cell cycle. It well established that ubiquitin-mediated degradations CDK inhibitors and cyclins are pivotal the timely unidirectional progression However, how itself modulated by degradation has been elusive. Here we report steady-state level controlled PAQR4, a member progestin adipoQ receptor family, SKP2, an E3 ubiquitin ligase. Knockdown PAQR4 leads to reduction proliferation, accompanied reduced protein CDK4. reduces polyubiquitination interacts...
Caspase-11, a cytosolic lipopolysaccharide (LPS) receptor, mediates lethal immune responses and coagulopathy in sepsis, leading cause of death worldwide with limited therapeutic options. We previously showed that over-activation caspase-11 is driven by hepatocyte-released high mobility group box 1 (HMGB1), which delivers extracellular LPS into the cytosol host cells during sepsis. Using phenotypic screening strategy recombinant HMGB1 peritoneal macrophages, we discovered FeTPPS, small...
Microtubule associated serine/threonine kinase-like (MASTL) is the functional mammalian ortholog of Greatwall kinase (Gwl), which was originally discovered in Drosophila. Gwl an essential for accurate chromosome condensation and mitotic progression, inhibits protein phosphatase 2A (PP2A), subsequently dephosphorylates substrates cyclin B1-cyclin-dependent 1, leading to exit. Previous studies have indicated that MASTL has a critical function regulation mitosis HeLa U2OS cell lines, though...
Abstract Background Lysosomal homeostasis and functions are essential for the survival of neural cells. Impaired lysosomal biogenesis acidification in Alzheimer’s disease (AD) pathogenesis leads to proteolytic dysfunction neurodegeneration. However, key regulatory factors mechanisms AD remain poorly understood. Methods ROCK1 expression its co-localization with LAMP1 SQSTM1/p62 were detected post-mortem brains healthy controls patients. Lysosome-related fluorescence probe staining,...
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease, characterized by phosphorylated TDP-43 (pTDP-43)-positive inclusions in neurons and glial cells. However, the pathogenic mechanism that underlies ALS remains largely unknown. To investigate effects of autophagy deficiency formation spreading pathological along corticospinal tract axons, preformed fibrils (PFFs) were prepared unilaterally injected into fifth layer left primary motor cortex (M1) or anterior horn...
Naïve B cells become activated and differentiate into antibody‐secreting plasma (PCs) when encountering antigens. Here, we reveal that the WW domain‐containing adapter protein with coiled‐coil (Wac), which is important for histone H2B ubiquitination (ubH2B), essential PC differentiation. We demonstrate cell‐specific Wac knockout mice have severely compromised T cell‐dependent ‐independent antibody responses. differentiation drastically despite undisturbed germinal center cell response in...
Abstract Background Parkinson's disease (PD) is a complex neurodegenerative disorder with strong genetic component. A growing number of variants and genes have been reported to be associated PD; however, there no database that integrate different type data, support analyzing PD-associated (PAGs). Methods By systematic review curation multiple lines public studies, we layers data (rare copy-number identified from patients PD, genome-wide association differential expression genes, DNA...
Spinocerebellar ataxia type 3 (SCA3) is an autosomal dominant inherited neurodegenerative disorder for which there currently no cure, nor effective treatment strategy.
Renal fibrosis is a common and irreversible pathological feature of end-stage renal disease caused by multiple etiologies. The role inflammation in tissue has been generally accepted. latest view that fatty acid metabolism disorder contributes to fibrosis. peroxisome proliferator activated receptor-gamma coactivator 1α (PGC1α) plays key metabolism, regulating uptake oxidized protein synthesis, preventing the accumulation lipid cytoplasm, maintaining dynamic balanced state intracellular...
Supplementary Figures 1-3 from Recombinant Immunoproapoptotic Proteins with Furin Site Can Translocate and Kill HER2-Positive Cancer Cells
Supplementary Figures 1-3 from Recombinant Immunoproapoptotic Proteins with Furin Site Can Translocate and Kill HER2-Positive Cancer Cells
<p>PDF file - 835K, 1. Reagents 2. Synthesis of cyclic peptide c(TNYL-RAW) (Lys-Thr-Asn-Tyr-Leu-Phe-Ser-Pro-Asn-Gly-Pro-ILe-Ala-Arg-Ala-Trp-Asp) 3. Radiolabeling HAuNS with 111In 4. SPR assay receptor binding affinity 5. Cell culture 6. Analysis EphB4 expression fig. S1. Physical properties HAuNS. Fig. S2. Binding and plasma stability c(TNYL-RAW). Western blot analysis immunostaining expression. S3. viability as a function equivalent DOX concentration. S4. Body weight change after the...
<p>PDF file - 835K, 1. Reagents 2. Synthesis of cyclic peptide c(TNYL-RAW) (Lys-Thr-Asn-Tyr-Leu-Phe-Ser-Pro-Asn-Gly-Pro-ILe-Ala-Arg-Ala-Trp-Asp) 3. Radiolabeling HAuNS with 111In 4. SPR assay receptor binding affinity 5. Cell culture 6. Analysis EphB4 expression fig. S1. Physical properties HAuNS. Fig. S2. Binding and plasma stability c(TNYL-RAW). Western blot analysis immunostaining expression. S3. viability as a function equivalent DOX concentration. S4. Body weight change after the...
To predict anastomotic occlusion after splenectomy combined with splenorenal shunt surgery by ultrasound technique. Methods: retrospectively analyze 53 cases of surgery. We divided these patients into 2 groups: a patency group (n=39) and an (n=14), which were based on the results venous stoma spiral CT. The statistical methods used to detection indicators (the internal diameter, blood flow velocity, volume, thrombosis direction portal vein, splenic superior mesenteric vein) for those groups,...