A5061727322- Inflammasome and immune disorders
- Motor Control and Adaptation
- Immune Response and Inflammation
- interferon and immune responses
- Cell death mechanisms and regulation
- Kawasaki Disease and Coronary Complications
- Action Observation and Synchronization
- IL-33, ST2, and ILC Pathways
- Muscle activation and electromyography studies
- Tactile and Sensory Interactions
- Gout, Hyperuricemia, Uric Acid
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- Virtual Reality Applications and Impacts
- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- Autoimmune and Inflammatory Disorders Research
- Phagocytosis and Immune Regulation
- COVID-19 Clinical Research Studies
- Cell Adhesion Molecules Research
- Trace Elements in Health
- Cardiac Valve Diseases and Treatments
- Sport Psychology and Performance
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Amyloidosis: Diagnosis, Treatment, Outcomes
Ehime University
2016-2025
Hiroshima Bunka Gakuen University
2017-2022
Bunka Gakuen University
2017-2022
Hyogo University of Teacher Education
2011-2015
Nagasaki Medical Center
2014
Shinshu University
2003-2014
National Hospital Organization
2014
Sasebo City General Hospital
2014
Nagasaki University Hospital
2014
Nagasaki University
2014
Background— Inflammation plays a key role in the pathophysiology of myocardial ischemia/reperfusion (I/R) injury; however, mechanism by which I/R induces inflammation remains unclear. Recent evidence indicates that sterile inflammatory response triggered tissue damage is mediated through multiple-protein complex called inflammasome. Therefore, we hypothesized inflammasome an initial sensor for danger signal(s) injury. Methods and Results— We demonstrate activation cardiac fibroblasts, but...
The cytoskeletal and/or nuclear matrix molecules responsible for morphological changes associated with apoptosis were identified using monoclonal antibodies (mAbs). We developed mAbs against Triton X-100-insoluble components of HL-60 cells pretreated all-trans retinoic acid. In particular, one mAb recognized a 22-kDa protein that exhibited intriguing behavior by forming an aggregate and appearing as speck during induced acid other anti-tumor drugs. Cloning sequencing its cDNA revealed this...
Several autoinflammatory disorders are associated with missense mutations within the nucleotide-binding oligomerization domain of cryopyrin. The mechanism by which cryopyrin cause inflammatory disease remains elusive. To understand molecular bases these diseases, we generated constructs to express three common disease-associated mutations, R260W, D303N, and E637G, compared their activity that wild-type protein. All mutant proteins tested were found induce potent NF-kappaB when This...
Nod1 is a member of family intracellular proteins that mediate host recognition bacterial peptidoglycan. To characterize immune responses mediated by Nod1, synthetic ligand compounds possessing enhanced ability to stimulate were developed study the function Nod1. Stimulation epithelial cells with stimulatory molecules induced chemokines and other proinflammatory are important for innate recruitment acute inflammatory cells. Administration ligands into mice cells, an activity was abolished in...
Abstract Apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC) is an adaptor molecule that has recently been implicated in the activation of caspase-1. We have studied role ASC host defense against intracellular pathogen Listeria monocytogenes. was found to be essential for secretion IL-1β/IL-18, but dispensable IL-6, TNF-α, and IFN-β production, macrophages infected with Listeria. Activation caspase-1 abolished ASC-deficient macrophages, whereas...
Familial Mediterranean fever (FMF) is a hereditary autoinflammatory disease that prevalent in populations. While it considered rare the rest of world, significant number FMF patients have been reported East Asia, including Japan. Our aim was to determine prevalence Japan and elucidate clinical genetic features Japanese patients. A primary nationwide survey conducted between January December 2009. Hospitals specializing pediatrics hospitals with pediatric, internal medicine,...
Alzheimer's disease is a neurodegenerative characterized by the interstitial deposition of amyloid β (Aβ) plaque, which thought to be related chronic neuroinflammation. Aβ known make fibrils via oligomers from monomers. has been reported activate NLRP3 inflammasome in infiltrated macrophages. NLRP3, an intracellular pattern recognition receptor, recognize numerous pathogens and/or metabolites and form complexes with adopter protein ASC inflammasome, interleukin (IL)-1β-processing platform....
Inflammatory cytokines such as interleukin (IL)-1 beta and IL-18 play an important role in the development of atherosclerosis restenosis. Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is adaptor that regulates caspase-1-dependent IL-1 generation; however, ASC vascular injury remains undefined. Here, we investigated contribution to neointimal formation after ASC-deficient (ASC(-/-)) mice.Wire-mediated was produced femoral artery ASC(-/-) wild-type mice....
Gastric gland mucin secreted from the lower portion of gastric mucosa contains unique O-linked oligosaccharides (O-glycans) having terminal α1,4-linked N-acetylglucosamine residues (αGlcNAc). Previously, we identified human α1,4-N-acetylglucosaminyltransferase (α4GnT), which is responsible for O-glycan biosynthesis and characterized αGlcNAc function in suppressing Helicobacter pylori vitro. In present study, engineered A4gnt–/– mice to better understand its role vivo. showed complete lack...
Familial Mediterranean fever (FMF) is an autoinflammatory disease caused by MEditerranean FeVer gene (MEFV) mutations. In Japan, patients with FMF have been previously reported, including a mild or incomplete form. Several factors are presumed to contribute the variable penetrance and phenotypic variability of FMF. We conducted current study investigate correlation clinical presentations MEFV genotypic distributions in Japanese patients. analyzed demographic, clinical, genetic data for 311...
Although Behçet's disease (BD) is a chronic inflammatory disorder of uncertain aetiology, the existence familial BD with autosomal-dominant traits suggests that responsibility gene (or genes) exists. We investigated Japanese family history to search for pathogenic mutations underlying biological mechanisms BD.6 patients over 4 generations who had suffered from frequent oral ulcers, genital ulcers and erythaema nodosum-like lesions in skin were assessed. Whole-exome sequencing was performed...
Bacterial pathogens utilize pore-forming toxins or sophisticated secretion systems to establish infection in hosts. Recognition of these system by nucleotide-binding oligomerization domain leucine-rich repeat proteins (NLRs) triggers the assembly inflammasomes, multiprotein complexes necessary for caspase-1 activation and maturation inflammatory cytokines such as IL-1β IL-18. Here we demonstrate that both NLRP3 NLRC4 inflammasomes are activated thermostable direct hemolysins (TDHs) type III...
The aim of this study was to evaluate the clinical manifestations and prevalence familial Mediterranean fever (FMF) in Japanese patients with unexplained rheumatic manifestations. We enrolled 601 or suspected FMF throughout Japan between 2009 2015. Patients were divided into three groups according Tel Hashomer criteria: sure FMF, probable non-FMF patients, including definitive diseases. Mutation detection exons 1, 2, 3, 10 gene MEFV performed by direct sequencing. A total 192 (31.9 %)...
The precise cytokine networks in the serum of individuals with familial Mediterranean fever (FMF) that are associated its pathogenesis have been unknown. Here, we attempted to identify specific biomarkers diagnose or assess disease activity FMF patients. We measured levels 45 cytokines 75 patients and 40 age-matched controls by multisuspension array. "attack" "remission" was classified Japan College Rheumatology-certified rheumatologists according Tel Hashomer criteria. Cytokines were ranked...
The pyrin domain was identified recently in multiple proteins that are associated with apoptosis and/or inflammation, but the physiological and molecular function of these remain poorly understood. We have Caspy Caspy2, two zebrafish caspases containing N-terminal domains. Expression Caspy2 induced mammalian cells were inhibited by general caspase inhibitors. Biochemical analysis revealed both active caspases, they exhibit different substrate specificity. Caspy, not interacted orthologue ASC...
Apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is pyrin N-terminal homology (PYD)- and (CARD)-containing proapoptotic molecule. This molecule has also been identified as target of methylation-induced silencing (TMS)-1. We cloned the ASC cDNA by immunoscreening using an anti-ASC monoclonal antibody. In this study, we determined binding site antibody on analyzed expression in normal human tissues. was observed anterior horn cells spinal cord, trophoblasts...
La autoantigen is a 47-kDa nuclear protein that binds to nascent polymerase III transcripts and number of viral RNAs. We show was cleaved generate 43-kDa fragment during apoptosis human leukemic HL-60 cells treated with camptothecin or etoposide. Immunofluorescence microscopy showed the level increased in cytoplasm cells. In addition, UV irradiation HeLa led cleavage redistribution upon apoptosis. Several lines evidence by caspase-3 closely related proteases at Asp-374 COOH terminus. When...
Gliomas are common tumors of the central nervous system, and majority patients with gliomas have a poor prognosis. The prediction prognosis is very important in selecting treatment. In present study, we retrospectively examined immunohistochemical staining cleaved caspase-3 (CC3), an activated form that acts as lethal protease at most distal stage apoptosis pathway, gliomas, correlation between activation to find useful prognostic indicators.Immunohistochemical CC3 was done 65 gliomas....