A5058773471- Cytokine Signaling Pathways and Interactions
- Lung Cancer Research Studies
- interferon and immune responses
- RNA modifications and cancer
- Synthesis and biological activity
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- Melanoma and MAPK Pathways
- Cancer therapeutics and mechanisms
- Bone health and treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Ovarian cancer diagnosis and treatment
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- HER2/EGFR in Cancer Research
- Health, Environment, Cognitive Aging
- Cancer Genomics and Diagnostics
- Glycosylation and Glycoproteins Research
- Cancer Immunotherapy and Biomarkers
- Respiratory viral infections research
- Epigenetics and DNA Methylation
- Lung Cancer Diagnosis and Treatment
- Ubiquitin and proteasome pathways
Osaka International Cancer Institute
2023-2025
Waseda University
2016-2023
National Institute of Advanced Industrial Science and Technology
2019-2023
National Cancer Research Institute
2023
Laboratoire d’immunologie intégrative du cancer
2022
Shiga University
2021
Shinshu University
2000-2017
Ōtani University
2005-2011
University of Glasgow
2011
Okayama University
2011
Abstract Activating innate immunity in cancer cells through cytoplasmic nucleic acid sensing pathways, a phenomenon known as “viral mimicry,” has emerged an effective strategy to convert immunologically “cold” tumors into “hot.” Through curated CRISPR-based screen of RNA helicases, we identified DExD/H-box helicase 9 (DHX9) potent repressor double-stranded (dsRNA) small cell lung cancers (SCLC). Depletion DHX9 induced accumulation dsRNA and triggered tumor-intrinsic immunity. Intriguingly,...
Phenotypic alterations in the lung epithelium have been widely implicated chronic obstructive pulmonary disease (COPD) pathogenesis, but precise mechanisms orchestrating this persistent inflammatory process remain unknown because of complexity parenchymal and mesenchymal architecture. To identify cell type–specific cell–cell interactions among multiple resident types cells that contribute to COPD progression, we profiled 57,918 from lungs patients with COPD, smokers without never-smokers...
Abstract Extracellular vesicles (EVs), including exosomes, are recognized as promising functional targets involved in disease mechanisms. However, the intravital heterogeneity of EVs remains unclear, and general limitation for analyzing is need a certain volume biofluids. Here, we present cellulose nanofiber (CNF) sheets to resolve these issues. We show that CNF capture preserve from ~10 μL biofluid enable analysis bioactive molecules inside EVs. By attaching moistened organs, collect trace...
Abstract DNA hypermethylated gene promoter sequences are extremely promising cancer markers. Their use for risk assessment, early diagnosis, or prognosis depends on the timing of this change during tumor progression. We studied proapoptotic ASC/TMS1 in lung and used findings to develop a sputum marker. protein levels reduced all types (30 40; 75%) but not 10 preinvasive lesions. Hypermethylation is also associated with invasive cancers (41 152 27.0% types) variation incidence between...
Abstract Small-cell lung cancer (SCLC) is the most lethal type of cancer. Paradoxically, this tumor displays an initial exquisite response to chemotherapy; however, at relapse, highly resistant subsequent available therapies. Here, we report that expression three prime repair exonuclease 1 (TREX1) strongly induced in chemoresistant SCLCs. Assay for transposase-accessible chromatin using sequencing and immunoprecipitation revealed a significant increase accessibility transcriptional activity...
Gliomas are common tumors of the central nervous system, and majority patients with gliomas have a poor prognosis. The prediction prognosis is very important in selecting treatment. In present study, we retrospectively examined immunohistochemical staining cleaved caspase-3 (CC3), an activated form that acts as lethal protease at most distal stage apoptosis pathway, gliomas, correlation between activation to find useful prognostic indicators.Immunohistochemical CC3 was done 65 gliomas....
Yes-associated protein (YAP) is a recently discovered growth-promoting transcription coactivator that has been shown to regulate the malignancy of various cancers. How YAP regulated not fully understood. Here, we show one factors regulating phosphatidylserine (PS) in recycling endosomes (REs). We use proximity biotinylation find proteins proximal PS. Among these are and multiple related signalling. Knockdown ATP8A1 (an RE PS-flippase) or evectin-2 RE-resident protein) masking PS cytoplasmic...
Rho GTPase Rac1 is a central regulator of F‐actin organization and signal transduction to control plasma membrane dynamics cell proliferation. Dysregulated activity often observed in various cancers including breast cancer suggested be critical for malignancy. Here, we showed that the ubiquitin E3 ligase complex Cullin‐3 (CUL3)/KCTD10 essential epidermal growth factor (EGF)‐induced/human receptor 2 (HER2)‐dependent activation HER2‐positive cells. EGF‐induced dorsal ruffle formation...
Abstract Purpose: Uterine leiomyosarcoma is among the most aggressive gynecological malignancies. No effective treatment strategies have been established. This study aimed to identify novel therapeutic targets for uterine based on transcriptome analysis and assess preclinical efficacy of drug candidates. Experimental Design: Transcriptome was performed using fresh-frozen samples six leiomyosarcomas three myomas. The Ingenuity Pathway Analysis (IPA) used potential target genes leiomyosarcoma....
Abstract Ductal carcinoma in situ (DCIS) is a precursor to invasive breast cancer. The frequency of DCIS increasing because routine mammography; however, the biological features and intratumoral heterogeneity remain obscure. To address this deficiency, we performed single-cell transcriptomic profiling ductal (IDC). was found be composed several transcriptionally distinct subpopulations cancer cells with specific functions. Several transcripts, including long noncoding RNAs, were highly...
Cell surface carbohydrates expressed on epithelial cells are thought to play an important role in tumor progression. Previously, we have shown that expression of core 2-branched O-glycans is closely correlated with vessel invasion and depth colon lung carcinomas. In this study, found 2 beta1,6-N-acetylglucosaminyltransferase-1, Core2GnT, positively the progression prostate cancer human patients. Statistical analysis demonstrated Core2GnT independent predictor for progressed pathological...
Uterine leiomyosarcoma (ULMS) is one of the most aggressive gynecological malignancies. In addition, molecular background ULMS has not been fully elucidated due to its low incidence. Therefore, no effective treatment strategies have established based on background. The present study aimed investigate roles microRNAs (miRNAs/miRs) in development ULMS. Comprehensive miRNA sequencing was performed using six and three myoma samples, revealed 53 11 significantly upregulated downregulated miRNAs,...
Abstract Small cell lung cancer (SCLC) is the most lethal type of cancer. The uniqueness this tumor lies in its initial exquisite response to chemotherapy, due high level genomic instability. However, patients experience relapse within 6 12 months. Although addition immune checkpoint blockade (ICB) therapy standard chemotherapy has been approved by FDA, only a small fraction SCLC responds immunotherapy because an immunologically “cold” microenvironment. Recently, using genetic depletion...
Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER)-localized transmembrane protein. STING induces the type I and inflammatory responses against a variety double-stranded DNA (dsDNA) viruses, which critical to limiting their infection replication. In certain settings where self-DNAs (genomic or mitochondrial DNA) emerge in cytosol intracellular membrane traffic impaired, becomes activated triggers inflammation, may contribute pathogenesis various autoinflammatory...
Bone is one of the most common metastatic sites breast cancer, and bone metastasis profoundly affects quality life cancer patients. commonly observed among all subtypes cancer; however, its molecular mechanism has been analyzed only in triple-negative subtype (TNBC). To characterize mechanisms luminal we established a bone-metastatic model MCF7, cell line, with enhanced osteolytic activity by intracaudal arterial injection (CAI). Pathological analysis lines revealed that they exhibited...
HER2 is overexpressed in 25-30% of breast cancers, and approximately 30% HER2-positive cancers metastasize to the brain. Although incidence brain metastasis cancer high, previous studies have been mainly based on cell lines triple-negative subtype, molecular mechanisms are unclear. In present study, we performed intracranial injection using nine evaluate their proliferative activity tissue. Our results show that UACC-893 MDA-MB-453 cells rapidly proliferated parenchyma, while other seven...
ABSTRACT Squamous cell carcinoma arising from mature teratoma (SCC‐MT) is a rare ovarian malignancy. The detailed molecular pathology of SCC‐MT not well understood. Moreover, the prognosis patients remains poor because no standard treatment has been established. In this study, we performed single‐nucleus RNA sequencing and spatial transcriptomics using clinical samples to identify novel therapeutic candidates. snRNA‐seq revealed three epithelial clusters, which one was significantly...
<p>Figure S4 shows TREX1 depletion increases sensitivity of resistant SCLC cells to chemotherapy</p>