A5015215943- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Endometrial and Cervical Cancer Treatments
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Cervical Cancer and HPV Research
- Cancer-related molecular mechanisms research
- Uterine Myomas and Treatments
- Gestational Trophoblastic Disease Studies
- Endometriosis Research and Treatment
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Cancer, Lipids, and Metabolism
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Renal and related cancers
- Inflammatory Biomarkers in Disease Prognosis
- PARP inhibition in cancer therapy
- Prenatal Screening and Diagnostics
- Colorectal and Anal Carcinomas
- Cancer Diagnosis and Treatment
- Intraperitoneal and Appendiceal Malignancies
- Gestational Diabetes Research and Management
- Maternal Mental Health During Pregnancy and Postpartum
Nagoya University
2016-2025
Nagoya University Hospital
2021-2025
Asahikawa University
2024
Chubu Rosai Hospital
2023
Cancer cell–derived extracellular vesicles (EVs) have unique protein profiles, making them promising targets as disease biomarkers. High-grade serous ovarian carcinoma (HGSOC) is the deadly subtype of epithelial cancer, and we aimed to identify HGSOC-specific membrane proteins. Small EVs (sEVs) medium/large (m/lEVs) from cell lines or patient serum ascites were analyzed by LC-MS/MS, revealing that both EV subtypes had proteomic characteristics. Multivalidation steps identified FRα,...
Endometriosis can cause of infertility, and evaluation methods for predicting clinical pregnancy outcomes are desired. Extracellular vesicles (EVs) exist in blood it contains small non-coding RNAs (ncRNAs) that may reflect disease severity. In this study, we investigated ncRNAs serum EVs to identify specific biomarkers pregnancy. Serum samples were collected from 48 patients who underwent assisted reproductive technology (ART). successfully isolated characterized using nanoparticle tracking...
Abstract The biological function of non-thermal atmospheric pressure plasma has been widely accepted in several types cancer. We previously developed plasma-activated medium (PAM) for clinical use, and demonstrated that PAM exhibits a metastasis-inhibitory effect on ovarian cancer through reduced MMP-9 secretion. However, the anti-tumor effects endometrial remain unknown. In this study, we investigated inhibitory cell viability vitro . Our results AMEC HEC50 viabilities were by at certain...
Abstract Extracellular vesicles (EVs), including exosomes, are recognized as promising functional targets involved in disease mechanisms. However, the intravital heterogeneity of EVs remains unclear, and general limitation for analyzing is need a certain volume biofluids. Here, we present cellulose nanofiber (CNF) sheets to resolve these issues. We show that CNF capture preserve from ~10 μL biofluid enable analysis bioactive molecules inside EVs. By attaching moistened organs, collect trace...
Cancer stem cells (CSCs) are a small cell subpopulation in many cancer types and involved various processes of tumor progression, such as initiation, metastasis recurrence. The distinguished features CSCs include variety biological properties, including self-renewal, multidifferentiation, stemness marker expression, resistance to chemotherapy radiotherapy. Despite their great potential clinical importance, the CSC signaling pathways not well understood at molecular level. MicroRNAs (miRNAs)...
Abstract Purpose: Uterine leiomyosarcoma is among the most aggressive gynecological malignancies. No effective treatment strategies have been established. This study aimed to identify novel therapeutic targets for uterine based on transcriptome analysis and assess preclinical efficacy of drug candidates. Experimental Design: Transcriptome was performed using fresh-frozen samples six leiomyosarcomas three myomas. The Ingenuity Pathway Analysis (IPA) used potential target genes leiomyosarcoma....
Abstract Background Assisted reproductive technology accounts for an increasing proportion of infertility treatments, and assessments to predict clinical pregnancy outcomes are desired. Extracellular vesicles exist in follicular fluid, small non coding RNAs extracellular underline the possibility reflecting potential. Methods Follicular fluid samples collected from 20 ovarian follicles 15 infertile patients undergoing assisted technology. isolated by serial centrifugation RNA sequencing is...
Abstract Aim Prenatal maternal depression is known to affect the neurodevelopment of offspring. This study aimed investigate profile umbilical cord serum in mothers with major depressive disorder (MDD). Methods Liquid chromatography–tandem mass spectrometry (LC–MS) was conducted using from MDD ( n = 5) and controls (control, 5). The levels several differentially expressed proteins were compared between 10) control groups by enzyme‐linked immunosorbent assay. Results proteomic profiles...
Uterine leiomyosarcoma (ULMS) is one of the most aggressive gynecological malignancies. In addition, molecular background ULMS has not been fully elucidated due to its low incidence. Therefore, no effective treatment strategies have established based on background. The present study aimed investigate roles microRNAs (miRNAs/miRs) in development ULMS. Comprehensive miRNA sequencing was performed using six and three myoma samples, revealed 53 11 significantly upregulated downregulated miRNAs,...
We focused on how the immunophenotypes based distribution of CD8-positive tumor-infiltrating lymphocytes (TILs) relate to endometrial cancer (EC) molecular subtypes and patients' prognosis.
Abstract Aim While manual vacuum aspiration (MVA) is commonly employed for early first‐trimester abortions, its effectiveness in treating hydatidiform mole still unclear. This study sought to evaluate the efficacy and safety of MVA comparison dilation curettage (D&C) managing mole. Methods We conducted a retrospective review medical records 198 patients with treated at Nagoya University Hospital between 2004 2023. After excluding cases incomplete data, we compared 106 who underwent...
ABSTRACT Squamous cell carcinoma arising from mature teratoma (SCC‐MT) is a rare ovarian malignancy. The detailed molecular pathology of SCC‐MT not well understood. Moreover, the prognosis patients remains poor because no standard treatment has been established. In this study, we performed single‐nucleus RNA sequencing and spatial transcriptomics using clinical samples to identify novel therapeutic candidates. snRNA‐seq revealed three epithelial clusters, which one was significantly...
Abstract [Objective] Ovarian cancer has poor prognosis due to its late diagnosis and the development of drug resistance, particularly in advanced stages. High-grade serous ovarian carcinoma (HGSOC), most common subtype, is characterized by extensive genomic alterations, including TP53 mutations copy number variations (CNVs). However, functional impact CNVs remains largely unclear. This study aimed investigate how influence extracellular vesicle (EV)-associated microRNA (miRNA) profiles...
<title>Abstract</title> Background Complete hydatidiform moles coexisting with a fetus (CHMCF) are uncommon. Although CHMCF is associated perinatal complications and post-molar gestational trophoblastic neoplasia (GTN), necessitating post-delivery chemotherapy, live birth remains feasible. This report presents 14 cases of in Japan. Methods We reviewed medical records patients treated at our hospital from 2000 to 2020 summarized clinical data, including maternal age, pregnancy details,...
MicroRNAs (miRNAs) are prospective biomarkers for diagnosis and prognosis due to their specific functions. Conventional methods lack the ability discriminate two main miRNA sources—extracellular vesicle (EV) miRNAs EV-free (i.e., not contained in EVs)—in one sample. Here, we demonstrate that a nanowire (NW) thermomicrofluidic device integrated with Peltier temperature controller can capture EVs then preferentially release by thermal energy extract EV via lysis buffer. This leads...
Abstract We determined the associations of follow-up blood pressure (BP) after stroke as a time-dependent covariate with risk subsequent ischemic stroke, well those BP levels difference in impact long-term clopidogrel or aspirin monotherapy versus additional cilostazol medication on secondary prevention. In sub-analysis randomized controlled trial (CSPS.com), patients between 8 and 180 days onset were randomly assigned to receive alone, combination clopidogrel. The percent changes,...
Extracellular vesicles (EVs) are present in body fluids and act as disease biomarkers. Emerging evidence has proven that EVs released not only from mammalian cells but also bacteria. Ovarian cancer a dismal prognosis because of difficulties early detection. This study aimed to identify bacterial EV (BEV) proteins associated with ovarian cancer. Fifteen patients or benign tumors were recruited, isolated the ascites. BEVs recovered seven vitro-cultured strains bacteria vaginal microbiota,...
There is increasing evidence that systemic inflammatory response (SIR) markers are prognostic factors for various types of cancers. This the first study to evaluate usefulness SIR prognosis early-stage ovarian clear-cell carcinoma (OCCC).We retrospectively investigated 83 patients diagnosed with stage I-II OCCC who underwent surgery between 2005 and 2017. Initially, receiver operating characteristic curve analysis overall survival (OS) was used determine optimal cut-off values...
Abstract Background This study was conducted to investigate the prognostic significance of sarcopenia in patients with organ metastatic cervical cancer. Methods Accordingly, data 40 cancer treated at our institute from December 2004 2017 were retrospectively analyzed. The correlation between clinicopathological characteristics and survival then evaluated using univariate multivariate analyses. Psoas muscle index (PMI), calculated psoas area L3 vertebral‐body level computed tomography images...
Abstract Ovarian clear cell carcinoma (OCCC) is a histological subtype of epithelial ovarian cancer and exhibits dismal prognosis due to chemoresistance. Moreover, only few effective therapeutic options exist for patients with recurrent OCCC, an understanding its molecular characteristics essential the development novel approaches. In present study, we investigated unique MicroRNAs (miRNA) profiles in recurrent/metastatic OCCC role miRNAs cisplatin resistance. Comprehensive miRNA sequencing...
High-grade serous ovarian carcinoma is a leading cause of death in female patients worldwide. MicroRNAs (miRNAs) are stable noncoding RNAs the peripheral blood that reflect patient's condition, and therefore, they have received substantial attention as noninvasive biomarkers various diseases. We previously reported usefulness serum miRNAs diagnostic biomarkers. Here, we investigated prognostic impact miRNA profile. used GSE106817 dataset, which included preoperative profiles with...