A5089578921- Lung Cancer Research Studies
- Peptidase Inhibition and Analysis
- Cancer therapeutics and mechanisms
- Microtubule and mitosis dynamics
- Diet and metabolism studies
- Diabetes and associated disorders
- Cellular transport and secretion
- Diabetes Management and Research
- Biochemical effects in animals
- Cytokine Signaling Pathways and Interactions
- Renal Diseases and Glomerulopathies
- Wnt/β-catenin signaling in development and cancer
- interferon and immune responses
- Adipose Tissue and Metabolism
- Diet, Metabolism, and Disease
- Pain Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Cellular Mechanics and Interactions
- Myofascial pain diagnosis and treatment
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and disorders
- Thyroid Cancer Diagnosis and Treatment
- Diabetes Treatment and Management
Sakhalin State University
2024
Fox Chase Cancer Center
2014-2024
Temple University Health System
2021
Vanderbilt University
2008-2011
Privolzhsky Research Medical University
2010
Vanderbilt University Medical Center
2007-2009
Bogomoletz Institute of Physiology
2000
Russian Academy of Sciences
1987-1988
Institute of Endocrinology
1986-1988
Abstract Activating innate immunity in cancer cells through cytoplasmic nucleic acid sensing pathways, a phenomenon known as “viral mimicry,” has emerged an effective strategy to convert immunologically “cold” tumors into “hot.” Through curated CRISPR-based screen of RNA helicases, we identified DExD/H-box helicase 9 (DHX9) potent repressor double-stranded (dsRNA) small cell lung cancers (SCLC). Depletion DHX9 induced accumulation dsRNA and triggered tumor-intrinsic immunity. Intriguingly,...
The hepatitis B virus (HBV) core protein is essential for HBV replication and an important target antiviral drug discovery. We report the first, to our knowledge, high-resolution crystal structure of compound bound protein. NVR-010-001-E2 can induce assembly wild-type Y132A mutant proteins thermostabilize with a Tm increase more than 10 °C. binds at dimer-dimer interface proteins, forms new interaction surface promoting protein-protein interaction, induces assembly, increases stability....
Epithelial-cadherin (E-cadherin) is a master organizer of the epithelial phenotype. Its function regulated in part by p120-catenin (referred to herein as p120), cytoplasmic binding partner that directly regulates cadherin stability. As it has been suggested cadherins have role inflammatory bowel disease (IBD), we sought investigate this further assessing effect p120 deficiency mouse small intestine and colon. conditional KO mice were superficially normal at birth but declined rapidly died...
An organized microtubule array is essential for the polarized motility of fibroblasts. Dynamic microtubules closely interact with focal adhesion sites in migrating cells. Here, we examined effect adhesions on dynamics. We observed that probability catastrophes (transitions from growth to shrinkage) was seven times higher at than elsewhere. Analysis dependence between rate and catastrophe throughout cytoplasm revealed a nonspecific (mechanical or spatial) factor provided minor contribution...
p120 loss destabilizes E-cadherin and could therefore result in tumor and/or metastasis-promoting activities similar to those caused by downregulation. Previously, we reported that is essential the intestine for barrier function, epithelial homeostasis survival. Conditional ablation mouse induced severe inflammatory bowel disease, but long-term cancer-related studies were impossible because none of animals survived longer than 21 days. Here, used a tamoxifen-inducible model...
Genome-Wide Association Studies (GWAS) and subsequent fine-mapping studies (>50) have implicated single nucleotide polymorphisms (SNPs) located at the CCDC170/C6ORF97-ESR1 locus (6q25.1) as being associated with risk of breast cancer. Surprisingly, our analysis using genome-wide differential allele-specific expression (DASE), an indicator for cancer susceptibility, suggested that genetic alterations CCDC170, but not ESR1, account GWAS-associated this locus. Breast cancer-associated CCDC170...
Mutations inactivating the cilia-localized Pkd1 protein result in autosomal dominant polycystic kidney disease (ADPKD), a serious inherited syndrome affecting ∼ 1 500 people, which accumulation of renal cysts eventually destroys function. Severity ADPKD varies throughout population, for reasons thought to involve differences both intragenic mutations and modifier alleles. The scaffolding NEDD9, commonly dysregulated during cancer progression, interacts with Aurora-A (AURKA) kinase control...
Enterobacteriaceae produce amyloid proteins called curli that are the major proteinaceous component of biofilms. Amyloids also produced by humans and associated with diseases such as Alzheimer's. During multistep process formation, monomeric subunits form oligomers, protofibrils, finally mature fibrils. Amyloid β oligomers more cytotoxic to cells than Oligomeric intermediates had not been previously detected. We determined turbulence inhibited biofilm formation that, intriguingly, aggregates...
Abstract Nestin, a class IV intermediate filament protein, is generally considered as putative marker of neural stem and progenitor cells in the central nervous system. Glioma common type adult brain tumors, glioblastoma (GBM) represents most aggressive form glioma. Here, we report that Nestin expression significantly upregulated human GBM, compared with other types knockdown or deletion U251 tumor from GBM patients derived xenografts resulted G2–M arrest, finally leading to apoptosis cells....
Directional cell migration requires polarization and asymmetric distribution of signaling. Focal adhesions microtubules are two systems which essential for these. It was shown that these closely interact with each other. is known microtubule targeting stimulates focal adhesion dissociation. Our recent study shows adhesions, in turn, specifically induce catastrophe via a biochemical mechanism. We were able to track down one the proteins paxillin involved this process. Paxillin phosphorylation...
We characterized a panel of cancer cells and found that they exhibited chromosome instability (CIN) was associated with high frequencies aberrant kinetochore:microtubule attachments. Failure to resolve these defective attachments before anaphase onset can lead missegregation chromosomes. Aurora B kinase is concentrated at the inner centromere where it contributes multiple kinetochore functions, one which in error-correction. Analysis several CIN cell lines showed many aspects functions were...
There was an error published in J. Cell Sci. 121, 196-204.The corresponding author regrets that a name omitted from the list and wishes to correctly acknowledge Ryoma Ohi for work presented this paper.
<div>Abstract<p>Activating innate immunity in cancer cells through cytoplasmic nucleic acid sensing pathways, a phenomenon known as “viral mimicry,” has emerged an effective strategy to convert immunologically “cold” tumors into “hot.” Through curated CRISPR-based screen of RNA helicases, we identified DExD/H-box helicase 9 (DHX9) potent repressor double-stranded (dsRNA) small cell lung cancers (SCLC). Depletion DHX9 induced accumulation dsRNA and triggered tumor-intrinsic...
<p>DHX9 suppresses ERE-derived dsRNA accumulation in SCLCs</p>
<p>Induction of IFN response after DHX9 loss in SCLC cell lines</p>