A5068046209- Hedgehog Signaling Pathway Studies
- Digestive system and related health
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Inflammasome and immune disorders
- Genomics and Chromatin Dynamics
- Pancreatic function and diabetes
- interferon and immune responses
- Helicobacter pylori-related gastroenterology studies
- Cancer-related gene regulation
- Genomic variations and chromosomal abnormalities
- RNA modifications and cancer
- Hemoglobinopathies and Related Disorders
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Tumors and Oncological Cases
- Pancreatic and Hepatic Oncology Research
- Congenital heart defects research
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Congenital gastrointestinal and neural anomalies
University of Michigan
2013-2025
Ann Arbor Center for Independent Living
2003-2016
Michigan United
2008-2009
Hacettepe University
2008
University of Alabama at Birmingham
2008
Hacettepe University Hospital
2008
Organogenesis (United States)
1999-2007
Wayne State University
1996-2002
Pennsylvania State University
1997
W.E. Upjohn Institute for Employment Research
1997
Villin, an actin bundling protein found in the apical brush border of absorptive tissues, is one first structural genes to be transcriptionally activated embryonic intestinal endoderm. In adult, villin broadly expressed every cell epithelium on both vertical axis (crypt villus tip) and horizontal (duodenum through colon) intestine. Here, we document that a 12.4-kilobase region mouse gene drives high level expression two different reporter (LacZ Cre recombinase) within entire transgenic mice....
Notch signaling is known to regulate the proliferation and differentiation of intestinal stem progenitor cells; however, direct cellular targets specific functions signals had not been identified. We show here in mice that directly crypt base columnar (CBC) cell maintain activity. inhibition induced rapid CBC loss, with reduced proliferation, apoptotic death efficiency organoid initiation. Furthermore, expression cell-specific marker Olfm4 was dependent on signaling, transcription activated...
Morphological development of the small intestinal mucosa involves stepwise remodeling a smooth-surfaced endodermal tube to form finger-like luminal projections (villi) and flask-shaped invaginations (crypts). These processes are orchestrated by instructive signals that pass bidirectionally between epithelium underlying mesenchyme. Sonic (Shh) Indian (Ihh) hedgehog expressed in throughout these morphogenic events, mice lacking either factor exhibit abnormalities. To examine combined role Shh...
Development of the asymmetric amniotic sac-with embryonic disc and ectoderm occupying opposite poles-is a vital milestone during human embryo implantation. Although essential to embryogenesis pregnancy, sac development in humans remains poorly understood. Here, we report pluripotent stem cell (hPSC)-based model, termed post-implantation embryoid (PASE), that recapitulates multiple embryogenic events centered around development. Without maternal or extraembryonic tissues, PASE self-organizes...
Patients with familial Mediterranean fever suffer sporadic inflammatory attacks characterized by and intense pain (in joints, abdomen, or chest). Pyrin, the product of the<i>MEFV</i> locus, is a cytosolic protein whose function unknown. Using pyrin as "bait" to probe yeast two-hybrid library made from neutrophil cDNA, we isolatedapoptotic speck containing caspase recruitment domain (CARD) (ASC), proapoptotic that induces formation large "specks" in transfected cells. We found when HeLa cells...
Tissue- and developmental stage-specific expression of the human beta-like globin genes is regulated by a combination ubiquitous erythroid-restricted trans factors that bind to cis elements near each five active genes. Additional interactions these with sequences located in far 5' end cluster occur as yet obscure mechanisms. Because complexity this regulatory puzzle, precise identification determinants control hemoglobin switching has proven difficult. Phylogenetic footprinting an...
In the adult intestine, an organized array of finger-like projections, called villi, provide enormous epithelial surface area for absorptive function. Villi first emerge at embryonic day (E) 14.5 from a previously flat luminal surface. Here, we analyze cell biology villus formation and examine role paracrine Hedgehog (Hh) signals in this process. We find that, before emergence, tight clusters Hh-responsive mesenchymal cells form just beneath epithelium. Cluster is dynamic; dorsally...
Epithelial-cadherin (E-cadherin) is a master organizer of the epithelial phenotype. Its function regulated in part by p120-catenin (referred to herein as p120), cytoplasmic binding partner that directly regulates cadherin stability. As it has been suggested cadherins have role inflammatory bowel disease (IBD), we sought investigate this further assessing effect p120 deficiency mouse small intestine and colon. conditional KO mice were superficially normal at birth but declined rapidly died...
In the intestine, finger-like villi provide abundant surface area for nutrient absorption. During murine villus development, epithelial Hedgehog signals promote aggregation of sub-epithelial mesenchymal clusters that drive emergence. Clusters arise first dorsally and proximally spread over entire intestine within 24-hours, but mechanism driving this pattern in is unknown. chick, driver cluster tensile force from developing smooth muscle, which generates deep longitudinal folds locally...
The cellular mechanisms that drive growth and remodeling of the early intestinal epithelium are poorly understood. Current dogma suggests murine fetal is stratified, villi formed by an epithelial process involving de novo formation apical surface at secondary lumina, radial intercalation stratified cells constitutes a major lengthening mechanism. Here, we investigate cell polarity, cycle dynamics shape in intestine between E12.5 E14.5. We show that, contrary to previous assumptions, this...
First identified in Drosophila, the Crumbs (Crb) proteins are important epithelial polarity, apical membrane formation, and tight junction (TJ) assembly. The conserved Crb intracellular region includes a FERM (band 4.1/ezrin/radixin/moesin) binding domain (FBD) whose mammalian partners not well understood PDZ motif that interacts with Pals1 (protein associated lin seven) (also known as MPP5). binds Patj (Pals1-associated tight-junction protein), multi-PDZ-domain protein associates many...
We demonstrate that dissociated human pluripotent stem cells (PSCs) are intrinsically programmed to form lumens. PSCs two-cell cysts with a shared apical domain within 20 hr of plating; these collapse monolayers after 5 days. Expression pluripotency markers is maintained throughout this time. In cysts, an domain, marked by EZRIN and atypical PKCζ, surrounded apically targeted organelles (early endosomes Golgi). Molecularly, actin polymerization, regulated ARP2/3 mammalian diaphanous-related...