A5000847126- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Clinical Nutrition and Gastroenterology
- Digestive system and related health
- 3D Printing in Biomedical Research
- Congenital gastrointestinal and neural anomalies
- Congenital Diaphragmatic Hernia Studies
- Congenital Anomalies and Fetal Surgery
- Neonatal Respiratory Health Research
- Fibroblast Growth Factor Research
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Intestinal Malrotation and Obstruction Disorders
- Tracheal and airway disorders
- Gastrointestinal motility and disorders
- Cancer Research and Treatments
- Angiogenesis and VEGF in Cancer
- Pediatric Hepatobiliary Diseases and Treatments
- Liver physiology and pathology
- Infant Nutrition and Health
- Pancreatitis Pathology and Treatment
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Urological Disorders and Treatments
- Gastrointestinal disorders and treatments
Children's Hospital of Los Angeles
2013-2023
University of Southern California
2013-2022
National Research Institute
2018
University of Colorado Boulder
2015
GTx (United States)
2012
Southern California University for Professional Studies
2008
Massachusetts General Hospital
2001-2005
Harvard University
2002-2004
Harvard University Press
2003
MaineGeneral Medical Center
2002
Endothelial-monocyte activating polypeptide II (EMAP II) was initially identified in the supernatant of murine methylcholanthrene A-induced fibrosarcomas (Meth A) by its capacity to activate host effector cells (Kao, J., Ryan, Brett, Chen, Shen, H., Fan, Y-G., Godman, G., Familletti, P., Wang, F., Pan, Y-C., Stern, D., and Clauss, M. (1992) J. Biol. Chem. 267, 20239-20247). Based on NH2-terminal protein sequence, a full-length cDNA has been cloned which indicates that precursor EMAP is...
ABSTRACT Short bowel syndrome (SBS) is characterized by poor nutrient absorption due to a deficit of healthy intestine. Current treatment practices rely on providing supportive medical therapy with parenteral nutrition; while life saving, such interventions are not curative and still associated significant co-morbidities. As approaches lengthen remaining intestinal tissue have been met only limited success transplants survival outcomes, new treating SBS necessary. Human intestine derived...
Tissue-engineered small intestine (TESI) has successfully been used to rescue Lewis rats after massive bowel resection. In this study, we transitioned the technique a mouse model, allowing investigation of processes involved during TESI formation through transgenic tools available in species. This is necessary step toward applying human therapy. Multicellular organoid units were derived from intestines mice and transplanted within abdomen on biodegradable polymers. Immunofluorescence...
During embryonic development, appropriate dorsoventral patterning of the trachea leads to formation periodic cartilage rings from ventral mesenchyme and continuous smooth muscle dorsal mesenchyme. In this work, we have investigated role two crucial morphogens, fibroblast growth factor 10 sonic hedgehog, in periodically alternating cartilaginous non-cartilaginous domains Using a combination gain- loss-of-function approaches for FGF10 SHH, demonstrate that precise spatio-temporal patterns...
In Brief Objective Novel production and in vitro characterization of tissue engineered colon. Summary Background Data The colon provides important functions short chain fatty acid production, sodium water absorption, storage. We report the first instance tissue-engineered (TEC) from autologous cells its characterization. Methods Organoid units, mesenchymal cell cores surrounded by a polarized epithelia derived full thickness sigmoid dissection neonatal Lewis rats, adult itself, were...
Abstract Dynamic regulation of intestinal cell differentiation is crucial for both homeostasis and the response to injury or inflammation. Sprouty2, an intracellular signaling regulator, controls pathways including PI3K MAPKs that are implicated in dysregulated inflammatory bowel disease. Here, we ask whether Sprouty2 secretory colitis. We report colonic epithelial deletion leads expanded tuft goblet populations. loss induces PI3K/Akt signaling, leading GSK3β inhibition interleukin (IL)-33...
Abstract Background The concept of disaster surge has arisen in recent years to describe the phenomenon severely increased demands on healthcare systems resulting from catastrophic mass casualty events (MCEs) such as natural disasters and terrorist attacks. major challenge dealing with a is efficient triage utilization resources appropriate magnitude character affected population terms its demographics types injuries that have been sustained. Results In this paper deterministic kinetics...
Fibroblast growth factors (FGFs) are a family of conserved peptides that play an important role in the development, homeostasis, and repair processes many organ systems, including gastrointestinal tract. All four FGF receptors several ligands present intestine. They roles controlling cell proliferation, differentiation, epithelial restitution, stem maintenance. Several FGFs have also been proven to be protective against diseases such as inflammatory bowel or aid regeneration after intestinal...
Intestinal epithelial cell renewal relies on the right balance of migration, proliferation, differentiation, and apoptosis. cells consist absorptive secretory lineage. The latter is comprised goblet, Paneth, enteroendocrine cells. Fibroblast growth factor 10 (FGF10) plays a central role in survival, differentiation several organs. expression pattern FGF10 its receptors both human mouse intestine their small have yet to be investigated. First, we analyzed FGF10, FGFR1, FGFR2, ileum throughout...
Loss of significant intestinal length from congenital anomaly or disease may lead to short bowel syndrome (SBS); failure be partially offset by a gain in epithelial surface area, termed adaptation. Current vivo models SBS are costly and technically challenging. Operative times survival rates have slowed extension transgenic models. We created new reproducible model zebrafish, tractable vertebrate model, facilitate investigation the mechanisms Proximal diversion at segment 1 (S1, equivalent...
Background Vascular endothelial growth factor (VEGF) is a highly conserved, master regulatory molecule required for cell proliferation, organization, migration and branching morphogenesis. Podocoryne carnea drosophila, which lack cells vascular system, express VEGF homologs, indicating potential roles beyond angiogenesis vasculogenesis. The role of in the development homeostasis postnatal small intestine unknown. We hypothesized regulating bioavailability would exhibit effects vasculature...
Using tissue-engineering techniques, we have developed a neointestine that regenerates the structural and dynamic features of native small intestine. In this study, tested neointestinal responsiveness to glucagon-like peptide 2 (GLP-2). Neointestinal cysts were engineered by seeding biodegradable polymers with neonatal rat intestinal organoid units. The matured anastomosed jejunum syngeneic adult recipients. Animals treated GLP-2 [Gly2] (twice daily, 1 microg/g body wt) or vehicle alone...