A5050676006- Topic Modeling
- Advanced Text Analysis Techniques
- Web Data Mining and Analysis
- Text and Document Classification Technologies
- Natural Language Processing Techniques
- Complex Network Analysis Techniques
- Sentiment Analysis and Opinion Mining
- Pituitary Gland Disorders and Treatments
- Diverse Approaches in Healthcare and Education Studies
- Health and Wellbeing Research
- Biomedical Text Mining and Ontologies
- Receptor Mechanisms and Signaling
- Educational Systems and Policies
- Education and Learning Interventions
- Spam and Phishing Detection
- Protein Kinase Regulation and GTPase Signaling
- Speech and dialogue systems
- Hippo pathway signaling and YAP/TAZ
- Neuropeptides and Animal Physiology
- AI in Service Interactions
- Machine Learning in Healthcare
- Psychosocial Factors Impacting Youth
- Recommender Systems and Techniques
- Sports injuries and prevention
- Misinformation and Its Impacts
University of Washington
2015-2024
University of Washington Medical Center
2024
Jeonbuk National University
2006-2020
Chonbuk National University Hospital
2013
Korea Research Institute of Bioscience and Biotechnology
1996-2011
ID Genomics (United States)
2005
National Institute of Informatics
2002-2003
Korea Advanced Institute of Science and Technology
2001
Ewha Womans University
1994
Mutations in the catalytic subunit of protein kinase A (PKAc) drive stress hormone disorder adrenal Cushing's syndrome. We define mechanisms action for PKAc-L205R and W196R variants. Proximity proteomic techniques demonstrate that both mutants are excluded from kinase-anchoring (AKAP)-signaling islands, whereas live-cell photoactivation microscopy reveals these indiscriminately diffuse throughout cell. Only cAMP analog drugs displace native PKAc AKAPs enhance cortisol release. Rescue...
The DNAJ-PKAc fusion kinase is a defining feature of fibrolamellar carcinoma (FLC). FLC tumors are notoriously resistant to standard chemotherapies, with aberrant activity assumed be contributing factor. By combining proximity proteomics, biochemical analyses, and live-cell photoactivation microscopy, we demonstrate that not constrained by A-kinase anchoring proteins. Consequently, the phosphorylates unique array substrates, including proteins involved in translation anti-apoptotic factor...
Adrenal Cushing's syndrome is a disease of cortisol hypersecretion often caused by mutations in protein kinase A catalytic subunit (PKAc). Using personalized medicine screening platform, we discovered driver mutation, PKAc-W196G, ~20% patient samples analyzed. Proximity proteomics and photokinetic imaging reveal that PKAc
Cushing's syndrome is an endocrine disorder caused by excess production of the stress hormone cortisol. Precision medicine strategies have identified single allele mutations within PRKACA gene that drive adrenal syndrome. These promote perturbations in catalytic core protein kinase A (PKAc) impair autoinhibition regulatory subunits and compartmentalization via recruitment into AKAP signaling islands. PKAcL205R found ∼45% patients, whereas PKAcE31V, PKAcW196R, L198insW C199insV insertion...
[Purpose] The purposes of this study were to examine whether the asymmetrical tonic neck reflex affects dominant-hand grip strength in healthy adults and compare results with degree achieved when Kinesio tape was applied flexor muscles dominant hands. [Subjects] subjects 20 men women who agreed participate study. [Methods] Grip measured using a Jamer dynamometer under 3 conditions: neutral position, head-neck system rotating toward nondominant hand transverse plane, after applying hand....
The authors studied psychiatric complications of disulfiram use in 605 alcoholic patients. subjects were assigned to one three treatment groups: a 250-mg group (N = 202), 1-mg 204) control for the fear alcohol-disulfiram reaction, and no-disulfiram 199) effect psychotherapy. No significant differences incidence found among groups. conclude that if follow use, must be very low with doses used presently absence predisposing factors.
Background: The ancient and highly evolutionarily conserved Wnt signaling pathway is critical in nearly all tissues organs for an organism to develop normally from embryo through adult. generally parsed into “canonical” or Wnt-β-catenin-dependent “non-canonical” β-catenin-independent signaling. Even though designating as either canonical noncanonical allows easier conceptual discourse about this pathway, fact non-canonical crosstalk regulates complex nonlinear networks. Objective: In...
Recent advances in proteomic technology reveal G-protein-coupled receptors (GPCRs) are organized as large, macromolecular protein complexes cell membranes, adding a new layer of intricacy to GPCR signaling. We previously reported the α1D-adrenergic receptor (ADRA1D)-a key regulator cardiovascular, urinary and CNS function-binds syntrophin family PDZ domain proteins (SNTA, SNTB1, SNTB2) through C-terminal ligand interaction, ensuring plasma membrane localization G-protein coupling. To assess...
This paper proposes a hybrid machine-translation system that combines neural machine translation with well-developed rule-based to utilize the stability of latter compensate for inadequacy in rare-resource domains. A classifier is introduced predict which from two systems more reliable. We explore set features reflect reliability and its process, training data automatically expanded small, human-labeled dataset solve insufficient-data problem. series experiments shows system’s accuracy...
An easy but robust strategy for the synthesis of bioderived polyelectrolyte nanogels protein antigen loading and vaccine adjuvant systems that can improve both humoral (Th2) cellular immunity (Th1) is presented. The synthesized promote uptake antigens into antigen-presenting cells strongly induce ovalbumin-specific INF-γ producing cells, cytotoxic T cell activity, antibody production.
Abstract Many G protein-coupled receptors (GPCRs) are organized as dynamic macromolecular complexes in human cells. Unraveling the structural determinants of unique GPCR may identify protein:protein interfaces to be exploited for drug development. We previously reported α 1D -adrenergic (α -ARs) – key regulators cardiovascular and central nervous system function form homodimeric, modular PDZ protein with cell-type specificity. Towards mapping -AR complex architecture, biolayer interferometry...
Abstract G protein-coupled receptor (GPCR) biogenesis, trafficking, and function are regulated by post-translational modifications, including N -glycosylation of asparagine residues. α 1D -adrenergic receptors (α -ARs) – key regulators central autonomic nervous system contain two putative sites within the large N-terminal domain at N65 N82. However, determining glycosylation state this has proven challenging. Towards understanding role these sites, site-directed mutagenesis lectin affinity...
Social media such as Twitter has come to reflect the reaction of general public major events. Since posts are short and noisy, it is hard extract reliable events based on word frequency. Even though an event term appears in a particularly low frequency, long at least one user mentions term, should be extracted. This paper proposes extraction method which combines reliability timeline analysis. The Latent Dirichlet Allocation (LDA) topic model adapted with weights terms users social detected...
In this paper, we propose a bias detection method that is based on personal and social opinions express contrasting views competing topics Twitter. We used unsupervised polarity classification conducted for learning targets. The <TEX>$tf{\cdot}idf$</TEX> algorithm applied to extract targets reflect sentiments features of tweets. Our addresses there being lack sentiment lexicon when opinions. To evaluate the effectiveness our method, experiments were four issues using Twitter test collection....
There have been many studies of automatic term recognition (ATR) and they achieved good results. However, focus on a mono-lingual extraction method. Therefore, it is difficult to extract terms from documents in foreign languages. This article describes an method languages using machine translation system. In our method, we translate into Korean the translated documents. Finally recognized are language. By one can for languages, which does not know.