A5016753875- Electron and X-Ray Spectroscopy Techniques
- Genomics and Chromatin Dynamics
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Calcium Carbonate Crystallization and Inhibition
- Quantum Dots Synthesis And Properties
- Advanced Materials Characterization Techniques
- Animal Genetics and Reproduction
- Nanoparticles: synthesis and applications
- Semiconductor Quantum Structures and Devices
- Marine Biology and Environmental Chemistry
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Plant Molecular Biology Research
- Trace Elements in Health
- RNA Interference and Gene Delivery
- Near-Field Optical Microscopy
- Gold and Silver Nanoparticles Synthesis and Applications
- Reproductive Biology and Fertility
- Advanced Materials and Mechanics
- Microfluidic and Bio-sensing Technologies
- Advanced Fluorescence Microscopy Techniques
- Semiconductor materials and devices
Northwestern University
2015-2024
In-Q-Tel
2013-2017
Northwestern University
2017
Imaging Center
2013
Minitube (United States)
2008
University of Wisconsin–Madison
2004-2008
Universität Ulm
1997-2007
Centro Interdisciplinar de Investigação Marinha e Ambiental
2004
Universidade do Porto
2002
Karlsruhe Institute of Technology
1999-2000
Fertilization-induced zinc sparks modify the structure and function of egg's zona pellucida, contributing to block polyspermy.
nano-ChIA combines multiple nanoimaging modalities to uncover 4D chromatin organization and its relationship molecular function.
Chromatin organization over multiple length scales plays a critical role in the regulation of transcription. Deciphering interplay these processes requires high-resolution, three-dimensional, quantitative imaging chromatin structure vitro. Herein, we introduce ChromSTEM, method that utilizes high-angle annular dark-field and tomography scanning transmission electron microscopy combined with DNA-specific staining for microscopy. We utilized ChromSTEM an in-depth quantification 3D conformation...
Not touching but sticking: By using cationic surfactant micelles as depletants, a directional entropic force approach (DEFA) assembles anisotropic nanoparticles into superlattices in solution. The induce the face-to-face stacking of to maximize system's entropy. shape determines symmetry superlattice, interparticle spacing is determined by charged surfactant. Learning how assemble inorganic ordered lattices may prove be important for applications, such as, electronics, photonics, and...
We report the development of thermoresponsive magnetic hydrogels based on poly(N-isopropylacrylamide) encapsulation Fe3O4 nanostructures (MNS). In particular, we examined effects encapsulated with poly-ethylene glycol (PEG) and polyhedral oligomeric silsesquioxane (POSS) surface modified MNS resonance (MR) T2 (transverse spin relaxation) contrast enhancement associated delivery efficacy absorbed therapeutic cargo. The microstructural characterization reveal regular spherical shape size (∼200...
Living tissues are an integrated, multiscale architecture consisting of dense cellular ensembles and extracellular matrices (ECMs). The cells ECMs cooperate to enable specialized mechanical properties dynamic responsiveness. However, the living difficult replicate. A particular challenge is identification a cell-like synthetic component, which tightly integrated with its matrix also responsive external stimuli. Here, we demonstrate that cellular-scale hydrated starch granules, underexplored...
1. The nutritional value of a pure algal food, the phytoflagellate Chlamydomonas reinhardtii, and mixed diet was tested for five planktonic cyclopoid copepods ( Acanthocyclops robustus , Cyclops vicinus, abyssorum Mesocyclops leuckarti Thermocyclops crassus ). algae were offered at high density (4.5 × 10 5 cells ml –1 ; 22.5 mg C l ) in flow‐through system. consisted concentrated natural plankton (rotifers, copepod nauplii, small copepodites large phytoplankton forms) size range 50–150 μm...
The endothelial cell (EC) lining of the pulmonary vascular system forms a semipermeable barrier between blood and interstitium regulates various critical biochemical functions. Collectively, it represents prototypical biomechanical system, where complex hierarchical architecture, from molecular scale to cellular tissue level, has an intimate intricate relationship with its biological We investigated mechanical properties human artery cells (ECs) using atomic force microscopy (AFM)....
Complementary tetrahedral small molecule-DNA hybrid (SMDH) building blocks have been combined to form nucleic acid-based polymeric nanoparticles without the need for an underlying template or scaffold. The sizes of these particles can be tailored in a facile fashion by adjusting assembly conditions such as SMDH concentration, time, and NaCl concentration. Notably, novel stabilized transformed into functionalized spherical acid (SNA) structures through incorporation capping DNA strands...
This manuscript describes cyclodextrin polymers formed as a thin coating on microcrystalline cellulose. The resulting polymer/cellulose composite shows promising performance for removing organic pollutants from water and can be packed into columns continuous-flow experiments. also excellent resistance to aerobic anaerobic biodegradation.
This work presents a new approach to study mechanisms and particle-size dependence of environmental release nanoparticles from polymer nanocompsites using fluorescent quantum dots.
By targeted disruption of the lactate dehydrogenase c (Ldhc) gene, we demonstrated that spermatozoa require Ldhc for capacitation, motility, and fertilizing capacity. expression is restricted to developing germ cells that, however, are apparently not compromised by lack LDHC isozyme. Because abundant in utilize aerobic glycolysis energy requirements, its main function was presumed be interconversion pyruvate with concomitant oxidation/reduction NADH NAD(+). We found sperm without were still...
Tooth enamel is a hard yet resilient biomaterial that derives its unique mechanical properties from decussating bundles of apatite crystals. To understand crystal nucleation and growth at nanoscale level to minimize preparation artifacts, the developing mouse matrix was imaged in situ using graphene liquid cells atomic resolution scanning transmission electron cryo-fracture microscopy. We report 1-2 nm diameter mineral precipitates aggregated form larger 5 particle assemblies within...
X-ray fluorescence nanotomography provides unprecedented sensitivity for studies of trace metal distributions in whole biological cells. Dose fractionation, which one acquires very low dose individual projections and then obtains high statistics reconstructions as signal from a voxel is brought together (Hegerl & Hoppe, 1976), requires accurate alignment these so to correct rotation stage runout. It shown here that differential phase contrast at 10.2 keV beam energy offers the potential...
Cryptococcus neoformans is a major human pathogen and cause of meningoencephalitis in immunocompromised patients. Many factors contribute to the extraordinary survivability pathogenicity this fungus humans, including copper homeostasis pathways. Previous work has shown that deletion copper-dependent regulator Cuf1 results decreased virulence dissemination brain infection, suggesting acquisition important persistence pathogen. Here, we show minimal quota C. maintained at high level even when...
The formation and resorption of CaCO(3) by epithelial cell layers require transport protons. We used the anterior sternal epithelium terrestrial isopod Porcellio scaber as a model to study expression pattern immunolocalization V-type H(+)-ATPase during mineralization demineralization intermittent deposits. Semiquantitative RT-PCR revealed that increases from non Ca(2+)-transporting control stages deposit resorption. In was larger in than posterior epithelium, which is not involved transports...