A5009386584- Microfluidic and Capillary Electrophoresis Applications
- Metabolomics and Mass Spectrometry Studies
- Electrochemical sensors and biosensors
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Biosensors and Analytical Detection
- Adipokines, Inflammation, and Metabolic Diseases
- Mass Spectrometry Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Electrochemical Analysis and Applications
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Apelin-related biomedical research
- Nanopore and Nanochannel Transport Studies
- Advanced Glycation End Products research
- Blood properties and coagulation
- Antioxidant Activity and Oxidative Stress
- Diabetes Management and Research
- Atherosclerosis and Cardiovascular Diseases
- Vitamin C and Antioxidants Research
- Regulation of Appetite and Obesity
- Analytical Chemistry and Chromatography
National Changhua University of Education
2007-2023
Texas A&M University
2004
Baylor College of Medicine
2004
Houston Methodist
2004
Severe obesity increases the prevalence of metabolic syndrome, and moderate acute weight loss with a very low-calorie diet in obese subjects syndrome leads to significant benefits. Adiponectin has been implicated both pathogenesis obesity-related insulin resistance increased inflammation. We analyzed relationship adipocyte-derived hormone adiponectin indices inflammation, adiposity, (MS+, n = 40) without (MS−, examined effects rapid loss. MS+ had significantly lower (7.6 ± 0.6 vs. 10.4...
Abstract A simple and fast micellar electrokinetic chromatography (MEKC) method was developed to investigate phospholipids isolated from human high‐density lipoproteins (HDL). To optimize the MEKC conditions, several factors including bile salt concentration organic modifier in separation buffer as well temperature have been examined. The optimal chosen a mixture of 50 mM salts, 30% v/v 1‐propanol 10 sodium phosphate (pH 8.5). applied voltage selected were 25 kV 40°C, respectively....
Surfactant‐coated multiwalled carbon nanotubes ( MWNT s) were used as pseudostationary phase PSP ) in CE to investigate the total lipids of high‐density lipoproteins and low‐density lipoproteins. To optimize conditions, several experimental factors including nanotube concentration, bile salt sodium phosphate PB organic modifier concentration buffer pH value have been examined. In addition, capillary temperature applied voltage also The optimal separation selected was a mixture 3.2 mg/L , 50...
C-Reactive protein (CRP) is a clinical biomarker of inflammation, and high levels CRP correlate with cardiovascular disease. The objectives this study were to test our hypothesis that oxidized low-density lipoprotein (ox-LDL) induces the release from human aortic endothelial cells (HAECs) optimize several analytical methods identify released cultured in model atherogenic stress. HAECs incubated copper-oxidized LDL, supernatant was subsequently purified by diethylaminoethyl chromatography...
Abstract The oxidized 1‐palmitoyl‐2‐arachidonoyl‐sn‐glycero‐3‐phosphocholine (ox‐PAPC) products in human high‐density lipoproteins (HDLs) were investigated by low‐flow capillary electrophoresis‐mass spectrometry (low‐flow CE‐MS). To accelerate the optimization, native PAPC (n‐PAPC) standard was first analyzed a commercial CE instrument with photodiode array detector. optimal separation buffer contained 60% (v/v) acetonitrile, 40% methanol, 20 mM ammonium acetate, 0.5% formic acid, and 0.1%...
ABSTRACT Phospholipids of in vitro oxidized human low‐density lipoproteins (LDL) were separated by two different solid‐phase extraction (SPE) methods. One the methods was designed to test effects gradient elution. This SPE method isolated more phospholipids from LDL than other one according results liquid chromatography and electrospray ionization mass spectrometry (LC ESI‐MS) analysis. A micellar electrokinetic capillary (MEKC) also used analyze SPE. The MEKC LC ESI‐MS consistent for major...
Abstract A simple CZE has been developed to investigate the binding reactions between C‐reactive protein (CRP) and native as well Cu 2+ oxidized phosphatidyl choline (PC) in this study. An aqueous‐organic solvent system containing 20% sodium phosphate, 70% methanol 10% acetonitrile was selected optimal separation buffer. Native PC showed a broad, higher mobility major peak sharp, lower minor peak. Oxidized (ox‐PC) appeared two adjacent peaks. bound CRP presence of metal ions Ca Mg . The...
A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak observed native, in vitro oxidized and glycated VLDL particles. The effective mobilities areas of the (CE) profiles showed good reproducibility precision. mobility higher than that native VLDL. similar Phospholipids isolated from were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a...
Abstract Capillary zone electrophoresis (CZE) has been utilized to profile the low‐density (LDL) particles in human blood serum this study. A 5 m M sodium phosphate buffer, pH 7.40, was chosen as most suitable CE buffer and an extensive ultrafiltration (UF) procedure applied purify LDL sample. Two particle species, with lower mobility − higher were observed. The electropherograms highly reproducible good precision of effective mobilities, corrected peak areas (CPAs) CPA ratio /LDL. shown on...
Abstract The apolipoproteins (APOs) of human very low‐density lipoprotein (VLDL) were investigated by an optimized cyclodextrin‐micellar electrokinetic chromatography (CD‐MEKC) method. separation buffer consisted 20 mM sodium phosphate, 40 bile salts (50% cholate and 50% deoxycholate), 25 carboxymethyl‐β‐cyclodextrin (CM‐β‐CD) (pH 7.0). For CD‐MEKC separation, a sample injection time 12 s, voltage 15 KV, capillary temperature 15°C chosen. optimal method showed good resolution repeatability...
Abstract Two approaches based on magnetic nanoparticles (MNPs) have been compared to analyze C‐reactive protein (CRP). Both the non‐eluted and eluted MNP‐1°Ab‐CRP‐2°Ab/FITC bioconjugates were measured by a microplate reader with fluorescence detection. The linear ranges for non‐elution elution methods 10‐200 0.1‐2.0 μg/mL, respectively. concentration limits of detection nonelution 2.91 0.04 non‐ method gave better precision recovery than method, also showed comparable results that ELISA...
Abstract Liquid‐phase and solid‐phase extractions (SPE) in combination with a simple micellar electrokinetic chromatography (MEKC) method were used to investigate human very low‐density lipoprotein (VLDL) lipids for two healthy donors. At absorbance 200 nm, the effective mobilities peak areas of MEKC profiles showed good reproducibility precisions. A major several minor peaks appeared total native VLDL, but both numbers reduced vitro oxidized VLDL. Two chloroform methanol fractions obtained...