A5100601456- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Assisted Reproductive Technology and Twin Pregnancy
- Heart Failure Treatment and Management
- Ovarian function and disorders
- Cardiac electrophysiology and arrhythmias
- PI3K/AKT/mTOR signaling in cancer
- Effects and risks of endocrine disrupting chemicals
- Genomic variations and chromosomal abnormalities
- Hepatitis C virus research
- Sperm and Testicular Function
- Sirtuins and Resveratrol in Medicine
- Pharmaceutical and Antibiotic Environmental Impacts
- Heavy Metal Exposure and Toxicity
- Hemoglobinopathies and Related Disorders
- Reproductive Health and Technologies
- Fetal and Pediatric Neurological Disorders
- Trace Elements in Health
- Cell Adhesion Molecules Research
- Liver Disease Diagnosis and Treatment
- Reproductive System and Pregnancy
- Cancer Genomics and Diagnostics
- Renal Diseases and Glomerulopathies
- Genetic and Kidney Cyst Diseases
- Endometriosis Research and Treatment
Southern Medical University
2013-2024
Nanfang Hospital
2014-2024
University of Hong Kong - Shenzhen Hospital
2023-2024
University of Hong Kong
2024
Nankai University
2021-2023
Tsinghua University
2023
Tianjin First Center Hospital
2022
Tianjin Medical University
2022
First Hospital of China Medical University
2020-2021
State Key Laboratory of Chemical Engineering
2021
Bisphenol A (BPA) is a widespread endocrine-disrupting chemical with estrogen like effects, which could interfere the human reproductive system by disrupting normal function of granulosa cells (GCs) leading to abnormal ovarian function. However, mechanism its toxicity on GCs has not been clearly described thus far.106 normogonadotropic infertile women undergoing their first in-vitro fertilization-embryo transfer (IVF-ET) cycle were recruited. Urinary BPA level and early outcomes IVF-ET...
The Na + - and Cl − -dependent GABA-betaine transporter (BGT1) has received attention mostly as a protector against osmolarity changes in the kidney potential controller of neurotransmitter GABA brain. Nevertheless, cellular distribution BGT1, its physiological importance, is not fully understood. Here we have quantified mRNA levels using TaqMan real-time PCR, produced number BGT1 antibodies, used these to study mice. (protein mRNA) predominantly expressed liver (sinusoidal hepatocyte plasma...
Maillard reaction (MR) is a non-enzymatic browning commonly seen in food processing, which occurs between reducing sugars and compounds with amino groups. Despite certain advantages based on products (MRPs) found some for health storage application have appeared, however, the MR occurring human physiological environment can produce advanced glycation end (AGEs) by modification of macromolecules such as proteins, lipids nucleic acid, could change structure functional activity molecules...
Abstract Objective To develop an accurate non‐invasive prenatal test using next generation sequencing (NGS) for HbE and the four most common β‐thalassaemia mutations found in South East Asia (namely −28A > G, CD17A T, CD41/42(−TTCT) IVS‐II‐654C T). Methods Cell‐free DNA was extracted from maternal plasma 83 families where both parents were carriers of mutation or one mutations. Overlapping PCR amplicons covering each generated, pooled sequenced Illumina MiSeq. Fastq files analysed to...
Persistent androgen receptor (AR) signaling is the key driving force behind progression and development of castration-resistant prostate cancer (CRPC). In many patients, AR COOH-terminal truncated splice variants (ARvs) play a critical role in contributing to resistance against depletion therapy. Unfortunately, clinically used antiandrogens like bicalutamide (BIC) enzalutamide (MDV), which target ligand binding domain, have failed suppress these variants. Here, we report for first time that...
Podocytes are key to the glomerular filtration barrier by forming a slit diaphragm between interdigitating foot processes; however, molecular details and functional importance of protein folding degradation in ER remain unknown. Here, we show that SEL1L-HRD1 complex ER-associated (ERAD) is required for formation function. ERAD highly expressed podocytes both mouse human kidneys. Mice with podocyte-specific Sel1L deficiency develop podocytopathy severe congenital nephrotic syndrome an...
To evaluate whether targeted sequencing and relative mutation dosage can be used to diagnose correctly inheritance of maternal β-thalassaemia mutations in cell-free DNA.Feasibility study using samples collected a prenatal clinic.South East Asia.Couples where both partners were known carriers one four common or an HbE mutation, therefore at risk carrying fetus affected with β-thalassaemia.49 previously identified as having inherited paternal amplified nested polymerase chain reaction (PCR),...
Phthalic acid esters are frequently detected in aquatic environments. In the present study, zebrafish were exposed to low concentrations (0 µg/L, 0.46 4.0 and 37.5 µg/L) of mono-(2-ethylhexyl) phthalate (MEHP) for 81 d, effects on reproduction, gamete quality, plasma vitellogenin (VTG), sex steroids, transcriptional profiles key genes involved steroidogenesis investigated. The results demonstrated that egg production sperm quality decreased after exposure MEHP, which also resulted reduced...
Natriuretic peptides, including B-type and C-type natriuretic peptide (BNP CNP), are powerful regulators of the cardiovascular system; however, their electrophysiological effects in heart, particularly sinoatrial node (SAN), incompletely understood. We have used high-resolution optical mapping to measure BNP CNP, roles receptors (NPR-A, NPR-B NPR-C), on electrical conduction within SAN atrial myocardium. In basal conditions CNP (50-500 nm) increased velocity (CV) by ∼30% at high dose shifted...
Triclosan (TCS) exists ubiquitously in the environment. Several vitro and vivo studies have demonstrated that TCS exerts endocrine disruptive effects on reproduction, but data from human populations are limited conflicting. The objective of our study was to investigate whether high urinary concentration is adversely associated with early reproductive outcomes women undergoing fertilization-embryo transfer (IVF-ET). This prospective cohort conducted September 2015 June 2016, including 156...
Sinoatrial node (SAN) disease mechanisms are poorly understood, and therapeutic options limited. Natriuretic peptide(s) (NP) cardioprotective hormones whose effects can be mediated partly by the NP receptor C (NPR-C). We investigated role of NPR-C in angiotensin II (Ang II)-mediated SAN mice. Ang caused due to impaired electrical activity myocytes increased fibrosis. Strikingly, treatment NPR-C-/- mice worsened disease, whereas co-treatment wild-type with a selective agonist (cANF) prevented...
Atrial natriuretic peptide (ANP) is a hormone with numerous beneficial cardiovascular effects. Recently, mutation in the ANP gene, which results generation of mutant form (mANP), was identified and shown to cause atrial fibrillation people. The mechanism(s) through mANP causes unknown. Our objective compare effects wild-type on electrophysiology mice humans.Action potentials (APs), L-type Ca(2+) currents (ICa,L), Na(+) current were recorded myocytes from or receptor C knockout (NPR-C(-/-))...
Cell-free DNA from maternal plasma can be used for non-invasive prenatal testing aneuploidies and single gene disorders, also has applications as a biomarker monitoring high-risk pregnancies, such those at risk of pre-eclampsia. On average, the fractional cell-free fetal concentration in is approximately 15%, but vary less than 4% to greater 30%. Although quantification straightforward case male fetus, there no universal marker; female fetus measurement more challenging. We have developed...
Small extracellular vesicles (sEVs) are important mediators of cell-to-cell communication involved in the successful establishment a pregnancy. Human decidual stromal cells play key role regulating trophoblast invasion. Nevertheless, regulatory functions cells-derived sEVs human still unclear. In this study, primary were isolated, and immortalized endometrial cell line (HESCs) decidualized into (HDSCs) using hormonal cocktail containing medroxy progesterone 17-acetate (MPA), estrogen cAMP...
The objective of the study was to detect aneuploidy in single fetal nucleated red blood cells (FNRBCs) from placental villi using whole genome amplification (WGA) and next generation sequencing.Three FNRBCs per sample were manually picked collected ten women undergoing elective first-trimester termination pregnancy, one or two each four aneuploid chorionic villus samples. Following WGA addition adaptor index sequences, samples sequenced on Illumina MiSeq. Leading trailing 15 bases trimmed,...
Abstract Circular dorsal ruffles (CDRs) are rounded membrane induced by growth factors to function as precursors of the large‐scale endocytosis called macropinocytosis. In addition their role in cellular uptake, recent research using cell line systems has shown that CDRs/macropinocytosis regulate canonical AKT–mTORC1 factor signaling pathway. However, CDRs have not been observed tissues, physiological relevance remained unclear. Here, utilizing ultrahigh‐resolution scanning electron...
Various factors have been reported to be associated with early miscarriages, including microbial infection, chemical toxicity, maternal disorders and genetic abnormalities. In the present study, a prospective cohort study was conducted investigate whether urinary concentrations of hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) were pregnancy loss in women undergoing vitro fertilization-embryo transfer (IVF-ET). Risk spontaneous patients exposed PAHs analysed using 40 who had...
Abstract Background Circular dorsal ruffles (CDRs) are rounded membrane induced on the surfaces of cells stimulated by growth factors (GF). They can serve as signal platforms to activate AKT protein kinase. After GF stimulation, phosphatidylinositol 3-kinase (PI3K) generates (3,4,5)-triphosphate (PIP3) in plasma membrane. PIP3 accumulates inside CDRs, recruits into structures, and phosphorylates them (pAKT). Given importance PI3K-AKT pathway signaling, CDRs likely involved cell growth....
Endometriosis (EM) is associated with oxidative stress. Advanced oxidation protein products (AOPPs) are novel markers of stress, which serve an important role as inflammatory mediator in various chronic diseases. In order to examine the AOPPs infertile women EM, present study analyzed levels AOPPs, estradiol (E2) and progesterone (P4) follicular fluid (FF) 89 or without EM undergoing vitro fertilization (IVF). The AOPP concentration FF group was significantly higher when compared that...
Background and Aims: Ravidasvir (RDV) is a new generation pangenotypic hepatitis C virus (HCV) NS5A inhibitor, with high barrier to baseline resistance-associated species. This the first phase 2/3 study conducted in Mainland China confirming efficacy safety of RDV + ritonavir-boosted danoprevir ribavirin for 12 weeks treatment-naïve noncirrhotic patients genotype 1 infection large population. Methods: In this multicenter, randomized, double-blinded, placebo-controlled trial (NCT03362814), we...
To explore whether resveratrol (Res) pretreatment could exert a protective effect on cyclophosphamide (Cy) induced ovarian toxicity in rat model.Twenty-four female 7-week old Sprague-Dawley rats were randomly divided into four groups: Con, administered with vehicle solutions; Cy, treated Cy; Res + Cy combined; Res, Res. After 21 d of treatments, the euthanized and blood samples collected to evaluate levels anti-Müllerian hormone (AMH). The Ovaries processed for immunohistochemical western...