A5101711065- Advanced Proteomics Techniques and Applications
- Sepsis Diagnosis and Treatment
- Colorectal Cancer Surgical Treatments
- Colorectal Cancer Screening and Detection
- Mass Spectrometry Techniques and Applications
- Cholesterol and Lipid Metabolism
- Hydrogen's biological and therapeutic effects
- Colorectal and Anal Carcinomas
- Computational Drug Discovery Methods
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Acute Ischemic Stroke Management
- Monoclonal and Polyclonal Antibodies Research
- Pesticide Exposure and Toxicity
- Pluripotent Stem Cells Research
- Optical Imaging and Spectroscopy Techniques
- Microfluidic and Bio-sensing Technologies
- Parkinson's Disease Mechanisms and Treatments
- Advanced Nanomaterials in Catalysis
- Phytoestrogen effects and research
- Nuclear Structure and Function
- Cellular Mechanics and Interactions
- Cancer-related gene regulation
- HER2/EGFR in Cancer Research
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
XinHua Hospital
2009-2025
Shanghai Jiao Tong University
2021-2025
State Key Laboratory of Oncogene and Related Genes
2023-2025
BeiGene (China)
2025
Sanya University
2024
Zhejiang University
2021-2024
Shanghai Ninth People's Hospital
2023
Ministry of Agriculture and Rural Affairs
2021
Qiqihar Medical University
2019
Colorectal Surgical Associates
2017
Identifying effective biomarkers has long been a persistent need for early diagnosis and targeted therapy of disease. While mass spectrometry-based label-free proteomics with trace cell demonstrated, deep ultratrace human biofluid remains challenging due to low protein concentration, extremely limited patient sample volume, substantial contact losses during preprocessing. Herein, we proposed validated lanthanide metal–organic framework flowers (MOF-flowers), as materials, trap enrich in...
Mechanism by which α-synuclein affects the progression of Parkinson's disease through Pyrin Domain Containing Protein 3 (NLRP3) was explored. Peripheral blood plasma 40 patients and normal healthy people attending department neurology Third Affiliated Hospital Qiqihar Medical University were collected from March 2018 to January 2019. The expression levels oligomers, phosphorylated α-synuclein, interleukin-1β (IL-1β), interleukin-6 (IL-6) transforming growth factor-α (TGF-α) in detected...
Label-free proteomics is widely used to identify disease mechanism and potential therapeutic targets. However, deep with ultratrace clinical specimen remains a major technical challenge due extensive contact loss during complex sample pretreatment. Here, hybrid of four boronic acid-rich lanthanide metal-organic frameworks (MOFs) high protein affinity introduced capture proteins in samples jointly by nitrogen-boronate complexation, cation-π ionic interactions. A MOFs Aided Sample Preparation...
BACKGROUND Fibrotic change is one of the important reasons for poor prognosis patients with acute respiratory distress syndrome (ARDS). The present study investigated effects hydrogen-rich saline, a selective hydroxyl radical scavenger, on lipopolysaccharide (LPS)-induced pulmonary fibrosis. MATERIAL AND METHODS Male ICR mice were divided randomly into 5 groups: Control, LPS-treated plus vehicle treatment, and saline (2.5, 5, or 10 ml/kg) treatment. Twenty-eight days later, fibrosis was...
Sepsis, a lethal organ dysfunction caused by dysregulated host response to infection, is the leading cause of worldwide in-hospital mortality. However, early diagnostic methods for sepsis are still urgent guiding accurate antibiotic usage and improving survival rate patients. Herein, we constructed PD-L1 antibody affinity microfluidic (PAAM) chip diagnosis severity assessment. The was used capture PD-L1-expressing leukocytes from whole blood samples obtained healthy control (HC) volunteers...
Drug-to-antibody ratio (DAR), free-drug-related impurities (FDRI) content, and purity are critical quality attributes of antibody-drug conjugates (ADCs), which substantially impact product safety efficacy. However, growing efforts in developing ADCs with higher complexity a faster timeline impose great challenge to their analytical support. Herein, for the ADC carries an antibody linker-payload distinct UV/vis absorption maxima, we propose high-throughput multiattribute analysis strategy...
Liquid-liquid phase separations (LLPS) are membraneless organelles driven by biomolecule assembly and implicated in cellular physiological activities. However, spatiotemporal deciphering of the dynamic proteome living cells during LLPS formation remains challenging. Here, we introduce Composition Assembly Proximity labeling-assisted Mass spectrometry (CLAPM). We demonstrate that CLAPM can instantaneously label monitor FUS interactome shifts within intracellular droplets undergoing LLPS....
This study was conducted to evaluate the effects of phytosterols (PS) and phytosterol esters (PSE) on C 57 BL/ 6 mice. Three groups 34 six-week-old mice specific pathogen free (SPF) grade, with an average initial body weight (IBW) 17.7g, were fed for 24 days either natural-ingredient diets without supplements or supplemented 89 mg/kg PS 400 PSE. Growth performance, blood biochemistry, liver colon morphology as well intestinal flora status evaluated. Both PSE exhibited growth promotion feed...
Background Laparoscopic sphincter-preserving low anterior resection for rectal cancer is a surgery demanding great skill. Immense efforts have been devoted to identifying factors that can predict operative difficulty, but the results are inconsistent. Objective Our study was conducted screen patients' build models predicting difficulty using well controlled data. Method We retrospectively reviewed records of 199 consecutive patients who had cancers 5–8 cm from anal verge. All underwent...
Our current study was conducted to identify patients' anatomic, pathologic, and clinical factors predict difficulty of performing laparoscopic abdominoperineal resection for ultra-low rectal cancer.Records 117 consecutive patients with cancer 2 5 cm from the anal verge were retrospectively reviewed. Using univariate multivariate linear or logistic regression models, standardized operative time blood loss, as well postoperative morbidity utilized endpoints screen multiple variables...
Abstract Thrombocytopenia is independently related with increased mortality in severe septic patients. Renin‐angiotensin system (RAS) elevated subjects; accumulating studies show that angiotensin II (Ang II) stimulate the intrinsic apoptosis pathway by promoting reactive oxygen species (ROS) production. However, mechanisms underlying relationship of platelet and RAS sepsis have not been fully elucidated. The present study aimed to elucidate whether was involved pathogenesis sepsis‐associated...
The aim of this study was to evaluate the impacts dietary phytosterol (PS) addition at different levels on serum parameters, nutrient digestibility and digestive enzyme activities white feather broilers. A total 600, 1-day-old male broilers were randomly divided into five groups with six replicates fed a basal diet supplemented 0 (control group), 10, 20, 40, or 80 mg PS/kg for 42 days. results showed that supplementation PS 20 40 mg/kg decreased malondialdehyde accumulation increased...
Photolithography is a widely used technology in microfabrication, where photomasks are mostly indispensable. The fabrication of generally costly, irreversible, and time-consuming, which limits the prompt delivery photolithography especially low-resource settings. Herein, we introduce facile technique for green reversible photomask with opaque droplets, termed as "droplet-based photomask." Paraffin oil, Span 80, water only components needed (total volume ∼1 mL). Specifically, microchip,...
Abstract Proteomic communications in neighboring microenvironments during early organ development is a dynamic process that continuously reshapes human embryonic stem cells (hESCs) developmental fate. Such proteomic alteration the microenvironment consists of both freely secreted proteome and exosome‐encapsulated proteome. Simultaneous monitoring time‐lapse shift proteomes with live organoids remains technically challenging. Here, c ontinuous o rganoid s ecretion/ e ncapsulation p roteome...
Tanshinones are a class of abietane diterpene compounds extracted from Salvia miltiorrhiza, and have been used for medical purposes in traditional Chinese medicinal practices. This herb has the treatment chronic obstructive pulmonary disease (COPD), breast cancer inflammatory disorders. study examined anti‑inflammatory properties tanshinones. In addition, lipid‑soluble which were specific to Tanshinone also highlighted, out two compounds, dihydrotanshinone I cryptotanshinone selected with...
<title>Abstract</title> The early-life organ development and maturation process shapes the fundamental blueprint for later-life phenotype. However, proteome atlas of self-multi-organs from infancy to adulthood is currently not available. Herein, we present a comprehensive proteomic analysis ten mice organs (brain, heart, lung, liver, kidney, spleen, stomach, intestine, muscle skin) acquired same individuals at three essential developmental stages (1-week, 4-week 8-week after birth) by...