A5041663727- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Analytical chemistry methods development
- X-ray Diffraction in Crystallography
- Bacteriophages and microbial interactions
- Crystallization and Solubility Studies
- Computational Drug Discovery Methods
- Cancer, Hypoxia, and Metabolism
- Heavy Metals in Plants
- Bacterial Genetics and Biotechnology
- Prostate Cancer Treatment and Research
- Heavy Metal Exposure and Toxicity
- Receptor Mechanisms and Signaling
- Enzyme Structure and Function
- Radioactivity and Radon Measurements
- Heavy metals in environment
- Amino Acid Enzymes and Metabolism
- RNA Interference and Gene Delivery
- Mass Spectrometry Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Mosquito-borne diseases and control
- Analytical Chemistry and Chromatography
- Mercury impact and mitigation studies
The University of Texas Medical Branch at Galveston
2015-2024
Durham VA Health Care System
2022-2023
Durham VA Medical Center
2021
John Sealy Hospital
2008-2019
University of Houston
2013-2018
Health & Safety Laboratory
2008-2017
Buxton Hospital
2017
Derbyshire County Council
2016
Galveston College
2008-2014
The University of Texas MD Anderson Cancer Center
2013
The xenobiotic metabolizing cytochromes P450 (P450s) are among the most versatile biological catalysts known, but knowledge of structural basis for their broad substrate specificity has been limited. 2B4 frequently used as an experimental model biochemical and biophysical studies these membrane proteins. A 1.6-Å crystal structure reveals a large open cleft that extends from protein surface directly to heme iron between α-helical β-sheet domains without perturbing overall fold. This is...
A 1.9-Å molecular structure of the microsomal cytochrome P450 2B4 with specific inhibitor 4-(4-chlorophenyl)imidazole (CPI) in active site was determined by x-ray crystallography. In contrast to previous experimentally structure, this complex adopted a closed conformation similar that observed for mammalian 2C enzymes. The differences between open and structures were primarily limited lid domain helices F through G, B′ C, N terminus helix I, β4 region. These large-scale conformational...
This update is a little shorter than in recent years, reflecting the fewer original papers published. However, trend for large number of review articles has continued and these cover wide range important topics. In 2012 update, noticeable increase work involving very small samples was evident. These observations continue through into current review. particularly seen with sample introduction systems used ICP-MS. A microflow device aerosol generation, based on thermal inkjet technology,...
Abstract Cancer cells display an increased demand for glucose. Therefore, identifying the specific aspects of glucose metabolism that are involved in pathogenesis cancer may uncover novel therapeutic nodes. Recently, there has been a renewed interest role pentose phosphate pathway cancer. This metabolic is advantageous rapidly growing because it provides nucleotide precursors and helps regenerate reducing agent NADPH, which can contribute to reactive oxygen species (ROS) scavenging....
The raging COVID-19 pandemic caused by SARS-CoV-2 has infected tens of millions people and killed several hundred thousand patients worldwide. Currently, there are no effective drugs or vaccines available for treating coronavirus infections. In this study, we have focused on the helicase (Nsp13), which is critical viral replication most conserved nonstructural protein within family. Using homology modeling that couples published electron-density with molecular dynamics (MD)-based structural...
Although checkpoint inhibitors that block CTLA-4 and PD-1 have improved cancer immunotherapies, targeting additional receptors may be required to broaden patient response immunotherapy. PVRIG is a coinhibitory receptor of the DNAM/TIGIT/CD96 nectin family binds PVRL2. We report antagonism TIGIT, but not CD96, increased CD8+ T-cell cytokine production cytotoxic activity. The inhibitory effect PVRL2 was mediated by demonstrating PVRIG-PVRL2 pathway nonredundant signaling node. A combination...
Background: Between August and November 2014, the incidence of Ebola virus disease (EVD) rose dramatically in several districts Sierra Leone.As a result, number cases exceeded capacity holding treatment centres.During December, additional beds were introduced, declined many areas.We aimed to measure patterns transmission different regions, evaluate whether bed is now sufficient meet future demand.Methods: We used mathematical model EVD infection estimate how extent nine worst affected Leone...
Abstract Retromer is a membrane coat complex that recruited to endosomes by the small GTPase Rab7 and sorting nexin 3. The timing of this interaction consequent endosomal dynamics are thought be regulated guanine nucleotide cycle Rab7. Here we demonstrate TBC1d5, GTPase-activating protein (GAP) for Rab7, high-affinity ligand retromer cargo selective VPS26/VPS29/VPS35. crystal structure TBC1d5 GAP domain bound VPS29 complementary biochemical cellular data show loop from binds conserved...
AR (androgen receptor) signaling is crucial for the development and maintenance of prostate as well initiation progression cancer. Despite AR's central role in cancer progression, it still unclear which AR-mediated processes drive disease. Here, we identified 4 core autophagy genes: ATG4B, ATG4D, ULK1, ULK2, addition to transcription factor TFEB, a master regulator lysosomal biogenesis function, transcriptional targets These findings were significant light our recent observation that...
Patterned substitution of d-amino acids into the primary sequences self-assembling peptides influences molecular-level packing and supramolecular morphology. We report that block heterochiral analogs model amphipathic peptide KFE8 (Ac-FKFEFKFE-NH
To better understand ligand-induced structural transitions in cytochrome P450 2B4, protein-ligand interactions were investigated using a bulky inhibitor. Bifonazole, broad spectrum antifungal agent, inhibits monooxygenase activity and induces type II binding 2B4dH(H226Y), modified enzyme previously crystallized the presence of 4-(4-chlorophenyl)imidazole (CPI). Isothermal titration calorimetry tryptophan fluorescence quenching indicate no significant burial protein apolar surface nor altered...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalysis of a metastable electronic excited state sodium nitroprusside by X-ray crystallographyMark R. Pressprich, Mark A. White, Yanina Vekhter, and Philip CoppensCite this: J. Am. Chem. Soc. 1994, 116, 12, 5233–5238Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June 1994https://pubs.acs.org/doi/10.1021/ja00091a030https://doi.org/10.1021/ja00091a030research-articleACS PublicationsRequest...
By converting cholesterol to 24S-hydroxycholesterol, cytochrome P450 46A1 (CYP46A1) initiates the major pathway for removal from brain. Two crystal structures of CYP46A1 were determined. First is 1.9-Å structure complexed with a high-affinity substrate 3-sulfate (CH-3S). The second that substrate-free at 2.4-Å resolution. CH-3S bound in productive orientation and occupies entire length banana-shaped hydrophobic active-site cavity. A unique helix B′–C loop insertion (residues 116–120)...
Despite the known importance of androgen receptor (AR) signaling in prostate cancer, processes downstream AR that drive disease development and progression remain poorly understood. This knowledge gap has thus limited ability to treat cancer. Here, it is demonstrated androgens increase metabolism glutamine cancer cells. was required for maximal cell growth under conditions serum starvation. Mechanistically, promoted by increasing expression transporters SLC1A4 SLC1A5, genes commonly...
Oridonin (1) has attracted considerable attention in recent years because of its unique and safe anticancer pharmacological profile. Nevertheless, it exhibits moderate to poor effects against highly aggressive cancers including triple-negative drug-resistant breast cancer cells. Herein, we report the rational design synthesis novel dienone derivatives with an additional α,β-unsaturated ketone system diversely installed A-ring based on this class natural scaffold that features dense...
This review discusses developments in elemental mass spectrometry, atomic absorption, emission and fluorescence, XRF LIBS, as applied to the analysis of specimens clinical interest, foods beverages. Sample preparation procedures quality assurance are also included.
Despite altered metabolism being an accepted hallmark of cancer, it is still not completely understood which signaling pathways regulate these processes. Given the central role androgen receptor (AR) in prostate we hypothesized that AR could promote cancer cell growth part through increasing glucose uptake via expression distinct transporters. Here, determined directly increased SLC2A12 , gene encodes transporter GLUT12. In support findings, signatures activity correlated with multiple...
A virally encoded ASCE ATPase motor ratchets between helical and planar configurations to translocate dsDNA into viral procapsids.