A5100378814- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Radiomics and Machine Learning in Medical Imaging
- MicroRNA in disease regulation
- Lung Cancer Diagnosis and Treatment
- Cardiac Imaging and Diagnostics
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Cardiovascular Disease and Adiposity
- Hepatocellular Carcinoma Treatment and Prognosis
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Gastric Cancer Management and Outcomes
- Organ Transplantation Techniques and Outcomes
- Coronary Interventions and Diagnostics
- Advanced X-ray and CT Imaging
- RNA modifications and cancer
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Cerebrovascular and Carotid Artery Diseases
- MRI in cancer diagnosis
- Intracranial Aneurysms: Treatment and Complications
Fourth Affiliated Hospital of Harbin Medical University
2014-2025
Harbin Medical University
2014-2025
Wenzhou Medical University
2024-2025
Guangdong Ocean University
2025
University of Electronic Science and Technology of China
2025
Quzhou University
2025
Shanghai University of Traditional Chinese Medicine
2012-2024
West China Hospital of Sichuan University
2018-2024
University Health Network
2015-2024
University of Toronto
2005-2024
A clinical trial that compared erlotinib with a placebo for non–small-cell lung cancer demonstrated survival benefit erlotinib. We used tumor-biopsy samples from participants in this to investigate whether responsiveness and its impact on were associated expression by the tumor of epidermal growth factor receptor (EGFR) EGFR gene amplification mutations.
To evaluate the effect of KRAS and epidermal growth factor receptor (EGFR) genotype on response to erlotinib treatment in BR.21, placebo-controlled trial.We analyzed 206 tumors for mutation, 204 EGFR 159 gene copy by fluorescent situ hybridization (FISH). We reanalyzed deletion/mutation using two highly sensitive techniques that detect abnormalities samples with 5% 10% tumor cellularity. mutation was direct sequencing.Thirty patients (15%) had mutations, 34 (17%) exon 19 deletion or 21 L858R...
<h2>Abstract</h2><h3>Purpose</h3> Interpretation systems for clinical laboratory reporting of genetic variants inherited conditions have been widely published. By contrast, there are no existing interpretation and classification somatic found from molecular testing cancer. <h3>Methods</h3> We designed an assessment protocol system identified through next-generation sequencing profiling tumor-derived samples applied these to a pilot dataset by 158 tumor derived advanced cancer patients...
Cathepsin B (CTSB) is a lysosomal protease that overexpressed in the early stage of many cancer types. Precise evaluation CTSB expression vivo may provide promising method for diagnosis cancers. By virtue high-resolution PA imaging modality, "smart" photoacoustic (PA) probe Cypate-CBT, which can self-assemble to cypate-containing nanoparticles response abundant GSH and inside tumor cells, was developed sensitive specific detection activity. Compared with unmodified Cypate, our Cypate-CBT...
The successes of targeted drugs with companion predictive biomarkers and the technological advances in gene sequencing have generated enthusiasm for evaluating personalized cancer medicine strategies using genomic profiling. We assessed feasibility incorporating real-time analysis somatic mutations within exons 19 genes into patient management. Blood, tumor biopsy archived samples were collected from 50 patients recruited four centers. Samples analyzed three technologies: exon Pacific...
Abstract Background We examine the potential prognostic and predictive roles of EGFR variant III mutation, gene copy number (GCN), human papillomavirus (HPV) infection, c-MET p16 INK4A protein expression in recurrent or metastatic squamous cell carcinoma head neck (R/M SCCHN). Methods analyzed archival tumor specimens 53 patients who were treated 4 phase II trials for R/M SCCHN. Two involved inhibitor erlotinib, 2 non-EGFR targeted agents. EGFRvIII mutation was determined by quantitative...
Hepatocellular carcinoma (HCC) is the third leading cause of cancer mortality worldwide. Many microRNAs (miRNAs), small non-coding RNAs, are involved in regulating cell proliferation, metastasis, migration, invasion and apoptosis. We investigated expression miR-135a HCC lines clinical tissues. The effect on migration HepG2 MHCC-97L were examined using wound healing Transwell assay. determined miR-135a, forkhead box O1 (FOXO1), matrix metalloproteinase-2 (MMP-2) Snail real-time PCR western...
Cyanidin-3-O-glucoside (C3G), an active ingredient in anthocyanins, mainly exists dark cereals. C3G was investigated for its effect on human gastric cancer (GC) cells, together with molecular mechanism. The CCK-8 assay results showed that had significant antiproliferative effects GC but it little normal cells. Western blot and flow cytometry regulated the reduction of mitochondrial membrane potential arrested cell cycle G2/M phase through AKT signaling pathway, causing cells to undergo...
Patients with type 2 diabetes mellitus (T2DM) are highly susceptible to cardiovascular disease, and coronary artery disease (CAD) is their leading cause of death. We aimed assess whether computed tomography (CT) based imaging parameters radiomic features pericoronary adipose tissue (PCAT) can improve the diagnostic efficacy patients T2DM have developed CAD.We retrospectively recruited 229 but no CAD history (146 were diagnosed at this visit 83 not). collected clinical information extracted...
The transcription factor E2F-1 drives proliferation and death, but the mechanisms that differentially regulate these divergent actions are poorly understood. hypoxia-inducible death Bnip3 is an target gene integral component of intrinsic mitochondrial pathway. govern activity remain cryptic. Herein we show NF-κB provides a molecular switch determines whether signals or under physiological conditions. We basal nonapoptotic conditions constitutively occupies transcriptionally silences by...
The incidence of acute kidney injury (AKI) is very high, and multiple physiopathological processes are involved, including endoplasmic reticulum stress (ERS). Tauroursodeoxycholic acid (TUDCA) an endogenous bile derivative that has been reported to inhibit ERS. To determine whether TUDCA had a nephroprotective effect on AKI explore the exact mechanism, ischaemia/reperfusion (I/R)-induced mouse model tunicamycin-pre-treated TCMK-1 cell were established. It was found renal tubular necrosis...
Hepatocellular carcinoma (HCC) is one of the most common human malignancies and third leading cause cancer mortality worldwide. The development progression HCC a complicated process, involving deregulation multiple genes that are essential to cell biological processes. Recently, microRNAs (miRNAs) have been suggested be closely associated with tumorigenesis. Our study showed miR-184 upregulated in lines tissues. Overexpression cells increased proliferation, tumorigenicity, cycle progression,...
Background MicroRNAs (miRNAs) are a recently discovered class of posttranscriptional regulators gene expression with critical functions in the angiogenesis and cardiovascular diseases; however, details miRNAs regulating mechanism ischemic cardiac microvascular endothelial cells (CMECs) not yet reported. Methods Results This study analyzes changes dynamic during process CMECs by applying miRNA chip real-time PCR for first time. Compared normal CMECs, have specific profile, which mir-223-3p...
The most common cause of death from prostate cancer (PCa) is metastases. There an increasing body evidence that microRNAs play important role in the development PCa by regulating target genes involved tumor metastasis. Here, we identified expression miR-486-5p was decreased metastatic C4-2 cells compared to non-metastatic LNCaP cells. Further validation clinical samples showed significantly tissues localized tissues. Functional studies demonstrated increased can suppress cell migration and...
Dysfunction of glucose metabolism is associated with the occurrence metabolic syndromes, including type 2 diabetes mellitus (T2DM). In this study, we investigated anti-diabetic effects yam aqueous extract and allantoin in high-fat-diet (HFD) streptozotocin (STZ)-induced diabetic mice mechanism action on dysfunction liver, pancreas, skeletal muscle. Male C57BL/6 were induced into a condition by HFD for 16 weeks single injection STZ (120 mg/kg) then orally administered (500 1000 or (20 50 once...
Wnt/β-catenin signaling pathway plays important roles in human cancer progression. Better understanding the mechanism underlying regulation of might provide novel therapeutic targets for treatment. miR-610 expression levels hepatocellular carcinoma (HCC) cell lines, HCC tissues and 76 archived specimens were determined using real-time PCR. Cell viability was measured by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. The level DNA synthesis BrdU incorporation Flow...