A5039647837- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Antibiotics Pharmacokinetics and Efficacy
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Animal Virus Infections Studies
- Influenza Virus Research Studies
- Infections and bacterial resistance
- interferon and immune responses
- Blood groups and transfusion
- Biosensors and Analytical Detection
- Aquaculture disease management and microbiota
- Genetic Mapping and Diversity in Plants and Animals
- Immune Response and Inflammation
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
Qingdao Agricultural University
2015-2024
University of Veterinary Medicine
2021
South China Agricultural University
2011-2016
The purpose of this study was to characterize a collection 103 multidrug resistance IncF plasmids recovered from Escherichia coli food producing and companion animals between 2003 2012. A total incF were characterized using an established PCR-based replicon sequence typing (RST) system identify FII, FIA, FIB (FAB) groups. Plasmids also analyzed using-restriction fragment length polymorphism (RFLP). Antibiotic Resistance determinants bla CTX-M , plasmid-mediated quinolone (PMQR) genes rmtB...
BackgroundThe association of PMQR and ESBLs in negative-bacteria isolates has been great concern. The present study was performed to investigate the prevalence co-transferability oqxAB blaCTX-M genes among 696 Escherichia coli (E. coli) from food-producing animals South China, characterize these plasmids. MethodsThe ESBL-encoding (blaCTX-M, blaTEM blaSHV), (qnrA, qnrB, qnrS, qnrC, qnrD, aac(6')-Ib-cr, qepA, oqxAB) were determined by PCR sequenced directionally. Conjugation, S1 nuclease...
To investigate the prevalence and genetic environment of multiresistance gene oqxAB in Salmonella enterica serotype Typhimurium isolated from food-producing animals. In this study, 63 isolates were analysed for presence plasmid-mediated quinolone resistance determinants mutations resistance-determining region by molecular methods (PCR/sequencing). The oqxAB-positive typed pulsed-field gel electrophoresis (PFGE). Plasmids carrying studied conjugation/transformation, replicon typing, Southern...
Vegetables harboring bacteria resistant to antibiotics are a growing food safety issue. However, data concerning carbapenem-resistant Enterobacteriaceae (CRE) in ready-to-eat fresh vegetables is still rare. In this study, 411 vegetable samples from 36 supermarkets or farmer's markets 18 cities China, were analyzed for CRE. Carbapenemase-encoding genes and other resistance among the CRE isolates. Plasmids carrying carbapenemase studied by conjugation, replicon typing, S1-PFGE southern blot,...
Tigecycline serves as one of the antibiotics last resort to treat multidrug-resistant (including carbapenem-resistant) pathogens. However, recently emerged plasmid-mediated tigecycline resistance mechanism, Tet(X), challenges clinical efficacy this class antibiotics. In study, we detected 180
ABSTRACT We report the presence of mcr-1 in Escherichia coli and carbapenem-resistant Cronobacter sakazakii from same diseased chicken. The gene linked with IS Apl1 was located on two different IncI2 plasmids, including one multidrug plasmid E. , whereas fosA3-bla NDM-9 an IncB/O C. . development resistance region mediated by 26 colocation or bla other genes will accelerate dissemination genes.
This study investigated the characteristics of Escherichia coli isolates carrying mcr-1-blaNDM from a chicken farm in China. Of 78 E. isolates, 21 clonally unrelated carried Diverse IncI2 plasmids disseminated mcr-1, while dissemination blaNDM was mediated by diverse IncB/O plasmids. More striking colocalization resistance genes mcr-1 and blaNDM-4 an IncHI2/ST3 plasmid, which might pose great challenge for public health.
In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 Escherichia coli isolates from diseased food-producing animals Guangdong province, China. The resistance-determining regions (QRDRs) gyrA and parC genes were analysed for mutations 55 harbouring only oqxAB all other PMQR genes. Overall, 282 (57.0 %) E. had at least one gene. detected 215 predominated genes, followed by qnrS (63 isolates), aac(6')-Ib-cr (56 qnrB (39 isolates) qepA (18...
Lincomycin is commonly used on swine farms for growth promotion as well disease treatment and control. Consequently, lincomycin may accumulate in the environment adjacent to many ways, thereby influencing antibiotic resistance environment. Levels of lincomycin-resistance genes residues water soil samples collected from multiple sites near wastewater discharge areas were investigated this study. Sixteen 16S rRNA detected using real-time PCR. Three genes, lnu(F), erm(A), erm(B), all except...
Background: The concurrence of mcr and carbapenemase genes among Enterobacteriaceae has been a great clinical concern. In our study, we aimed to investigate the prevalence -positive carbapenem-resistant (CRE) in fresh vegetables shed light on possibility transmission CRE via vegetables. Methods: this 712 vegetable samples from 10 provinces China were collected between May 2017 Dec 2018 screened for genes. Antibiotic susceptibilities isolates co-harboring determined by an agar dilution or...
Chicken products and chickens with colibacillosis are often reported to be a suspected source of extraintestinal pathogenic Escherichia coli (ExPEC) causing several diseases in humans. Such pathogens healthy can also contaminate chicken carcasses at the slaughter then transmitted humans via food supply; however, reports about ExPEC still rare. In this study, we determined prevalence characteristics isolates China. A total 926 E. from seven layer farms (371 isolates), one white-feather...
The global spread of colistin or carbapenem-resistant Enterobacteriaceae (CRE) has been a pressing threat to public health. Members Enterobacteriaceae, especially Proteus mirabilis and Escherichia coli, have prevalent foodborne pathogens such from fresh vegetables triggered illness in China. However, reports about CRE, P. vegetables, are still lacking. In this study, we identified five blaNDM-positive generic E. coli concurrently two markets China, four the also carried mcr-1. 10 isolates...
Lincomycin, chlortetracycline, and amoxicillin are commonly used antimicrobials for growth promotion infectious disease prophylaxis in swine production. In this study, we investigated the shifts resistance development among intestinal microbiota pregnant sows before after lincomycin, treatment by using phylogenetic analysis, bacterial enumeration, PCR. After antimicrobial treatment, microbial community, an increased proportion of resistant bacteria, genes related to as compared day...
The emergence and spread of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) is causing worldwide concern, whereas NDM-producing hvKP still rare. Here we report the complete genome sequence characteristics an NDM-1-producing ST23 type clinical in PR China.Capsular polysaccharide serotyping was performed by PCR. isolate 3214 obtained using both Illumina Hiseq platform Pacbio RS platform. Multilocus identified submitting to mlst 2.0 antimicrobial resistance genes plasmid...
Streptococcus suis isolates from diseased pigs were examined for susceptibility to nine antimicrobials, possession of virulence-associated factors (VFs), and distribution serotypes. The association between antimicrobial resistance (AMR) serotypes as well VFs was subsequently assessed. Among the investigated, serotype 2 (66.04%) mostly prevalent, followed by 1 (23.27%), 9 (1.26%), 7 (0.63%), whereas 14 untypable polymerase chain reaction typing method used. Analysis with pulsed-field gel...
qnrD, unlike other qnr genes, is mainly located on small nonconjugative plasmids. We investigated the presence of qnrD among 1,373 Enterobacteriaceae isolates in China. Twelve qnrD-positive strains were detected, and all nonsusceptible to fluoroquinolones. The complete sequence plasmids showed that determinants two with a respective size ∼4.2 2.7 k-bp. Interestingly, identification this study revealed highest prevalence Proteeae identified.
Plasmid-mediated quinolone-resistance (PMQR) genes were determined by polymerase chain reactions (PCRs) in 250 Escherichia coli isolates from food-producing animals Guangdong, China, 2009-2010. Then, the prevalence of plasmid-mediated β-lactamase and 16S rRNA methylase was PCRs among PMQR-positive isolates. One hundred fifty-seven (62.8%) found to harbor at least one PMQR gene, qnrS (84) oqxAB (97) most two prevalent genes. (ESBL and/or AmpC type) detected 106 157 strains. The bla(TEM-1)...