A5023461978- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Antibiotics Pharmacokinetics and Efficacy
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Bacterial Identification and Susceptibility Testing
- Infections and bacterial resistance
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Identification and Quantification in Food
- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Dermatology and Skin Diseases
- Food composition and properties
- Aquaculture disease management and microbiota
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Enterobacteriaceae and Cronobacter Research
- Nail Diseases and Treatments
- Phytochemicals and Antioxidant Activities
- Peanut Plant Research Studies
- Allergic Rhinitis and Sensitization
- Tea Polyphenols and Effects
Wenzhou Medical University
2024
South China Agricultural University
2019-2023
Henan University of Technology
2022-2023
Key Laboratory of Guangdong Province
2020-2022
Yangzhou University
2021
Abstract Background The recent emergence and dissemination of high-level mobile tigecycline resistance Tet(X) challenge the clinical effectiveness tigecycline, one last-resort therapeutic options for complicated infections caused by multidrug-resistant Gram-negative Gram-positive pathogens. Although tet (X) has been found in various bacterial species, less is known about phylogeographic distribution phenotypic variance different genetic variants. Methods Herein, we conducted a multiregional...
Tigecycline serves as one of the antibiotics last resort to treat multidrug-resistant (including carbapenem-resistant) pathogens. However, recently emerged plasmid-mediated tigecycline resistance mechanism, Tet(X), challenges clinical efficacy this class antibiotics. In study, we detected 180
Plasmid-mediated antimicrobial resistance has emerged as one of the principal global issues, posing significant threats to public health. Herein, we reported a mobile tigecycline mechanism Tet(X4) on both plasmid and chromosome in Escherichia coli strains from migratory birds China. Besides tigecycline, these tet(X4)-positive also exhibited elevated MICs FDA newly approved tetracycline antibiotics, eravacycline (4 µg/ml) omadacycline (8 µg/ml). Worrisomely, tet(X4)-carrying plasmids shared...
The emergence of the plasmid-mediated high-level tigecycline resistance mechanism Tet(X) threatens role as "last-resort" antibiotic in treatment infections caused by carbapenem-resistant Gram-negative bacteria. Compared with that prototypical Tet(X), enzymatic activities Tet(X3) and Tet(X4) were significantly enhanced, correlating resistance, but underlying mechanisms remain unclear. In this study, we probed key amino acid changes leading to enhancement function clarified structural...
Abstract Antimicrobial resistance (AMR) is a major threat to global public health, and antibiotic genes (ARGs) are widely distributed across humans, animals, environment. Farming environments emerging as key research area for ARGs resistant bacteria (ARB). While the skin an important reservoir of ARB, transmission mechanisms between farming human remain unclear. Previous studies confirmed that swine farm environmental exposures alter microbiome, but timeline these changes ill defined. To...
The emergence of the tigecycline resistance gene tet (X) has posed a severe threat to public health. However, reports its origin and distribution in human remain rare.
Abstract Objectives The emergence and spread of plasmid-encoded tet(X3/X4) genes that confer high-level tigecycline eravacycline resistance in Escherichia coli Acinetobacter spp. pose serious threats to human animal health. We developed a rapid robust assay detect Tet(X3/X4) Gram-negative bacteria based on degradation by the presence Tet(X) enzyme test strain. Methods This tetracycline inactivation method (TIM) is Tet(X3/X4)-producing strain, which results reduced activity against an...
Carbapenems, colistin, and tigecycline are critically important antibiotics in clinics. After the global appearance of blaNDM mcr mediating resistance to carbapenems respectively, becomes last-resort drug against severe human infections caused by multidrug-resistant bacteria. Recently, a mobile gene tet(X4) has been identified Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii that causes high other tetracyclines. In this study, prevalence E. coli isolates from duck goose farms...
The emergence and spread of the novel mobile Tet(X) tetracycline destructases confer high-level tigecycline eravacycline resistance in Escherichia coli Acinetobacter spp. pose serious threats to human animal health. Therefore, a rapid robust detection assay was urgently needed monitor dissemination resistance. We developed simple detect producers Gram-negative bacteria based on matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS). This MALDITet(X) test...
We reported the complete nucleotide sequence of a tet (X4)-carrying plasmid, pSTB20-1T, from tigecycline-resistant Escherichia coli isolate in China. Sequence analysis indicated that pSTB20-1T contains hybrid plasmid backbone and (X4)-containing multidrug resistance region, likely originated through recombination multiple plasmids. (X4) was flanked by two IS CR2 , which may be responsible for mobilization.
Abstract Catechin (EC) is a natural plant extract and has great health benefits. EC interacts with the starch isolated from Chinese chestnut to form complexes. The effect of addition on structure retrogradation properties (CS) investigated in this study. Compared CS, when 10%, peak viscosity (PV), trough viscosity(TV), setback (SB) CS decreased by 45.65%, 39.95%, 47.38% (2185.62, 922.35, 445.42 cp), respectively. Thermogravimetric shows that can decrease degradation temperature starch....
The widespread of tetracycline (TC) residues in anthropogenic and natural environments pose an immediate threat to public health. Herein, we established the TET-Yeasate, approach based on whole-cell lysate engineered yeast, mitigate TC contamination environment. TET-Yeasate is defined as biological matrix whole cell from yeast that containing TC-degradative components (Tet(X), NADPH, Mg2+) protective macromolecules. was able efficiently eliminate tap water (98.8%), lake (77.6%), livestock...
The dissemination of strains producing tetracyclines monooxygenase Tet(X) from breeding farms to the natural environment poses a potential threat public health.
Campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. Consumption chicken meat considered main route for human infection with Campylobacter. This study aimed to determine critical factors Campylobacter cross-contamination in Chinese commercial kitchens during handling. Five were visited detect occurrence from 2019 2020. Chicken samples (n = 363) and cotton balls kitchen surfaces 479) collected, total counts spp. detected. Genotypic characterization 57 jejuni...
The rapid dissemination of plasmid-mediated tet (X) genes in Acinetobacter species has compromised the clinical effectiveness tigecycline, one last-resort antibiotics. However, classification strategy and homology group (X)-positive spp. plasmids remain largely unknown. In this study, we classified them by genome-based replicon typing, followed analyses structural characteristics, transferability vivo effect. A total 34 distributed at least nine were collected, including three (X3)-positive...
Abstract The emergence of tet (X) genes has compromised the clinical use last-line antibiotic tigecycline. We identified 322 (1.21%) positive samples from 12,829 human microbiome distributed in four continents (Asia, Europe, North America and South America) using retrospective data worldwide. These were dominated by (X2)-like orthologs but we also 12 carrying novel genes, designed (X15) (X16), that resistant to metagenomic analysis revealed these anaerobes Bacteroidaceae (78.89%) human-gut...