A5001039525- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Essential Oils and Antimicrobial Activity
- Antibiotic Use and Resistance
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Veterinary medicine and infectious diseases
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- Urinary Tract Infections Management
- Natural product bioactivities and synthesis
- Biosensors and Analytical Detection
- Clostridium difficile and Clostridium perfringens research
- Parasitic Infections and Diagnostics
South China Agricultural University
2013-2023
Yangzhou University
2021-2022
Key Laboratory of Guangdong Province
2020-2021
Anthropogenic environments have been implicated in enrichment and exchange of antibiotic resistance genes bacteria. Here we study the impact confined controlled swine farm on temporal changes gut microbiome resistome veterinary students with occupational exposure for 3 months. By analyzing 16S rRNA whole metagenome shotgun sequencing data tandem culture-based methods, show that shapes students, resulting potentially pathogenic taxa antimicrobial genes. Comparison students' microbiomes...
The purpose of this study was to characterize a collection 103 multidrug resistance IncF plasmids recovered from Escherichia coli food producing and companion animals between 2003 2012. A total incF were characterized using an established PCR-based replicon sequence typing (RST) system identify FII, FIA, FIB (FAB) groups. Plasmids also analyzed using-restriction fragment length polymorphism (RFLP). Antibiotic Resistance determinants bla CTX-M , plasmid-mediated quinolone (PMQR) genes rmtB...
Combating plasmid-mediated carbapenem resistance is essential to control and prevent the dissemination of carbapenem-resistant Enterobacteriaceae (CRE). Here, we conducted a proof-of-concept study demonstrate that CRISPR-Cas9-mediated gene plasmid curing can effectively resensitize CRE carbapenems. A novel plasmid-curing system (pCasCure) was developed electrotransferred into various clinical isolates. The results showed pCasCure cure bla KPC , NDM OXA-48 in species Klebsiella pneumoniae...
Abstract Background The recent emergence and dissemination of high-level mobile tigecycline resistance Tet(X) challenge the clinical effectiveness tigecycline, one last-resort therapeutic options for complicated infections caused by multidrug-resistant Gram-negative Gram-positive pathogens. Although tet (X) has been found in various bacterial species, less is known about phylogeographic distribution phenotypic variance different genetic variants. Methods Herein, we conducted a multiregional...
BackgroundThe association of PMQR and ESBLs in negative-bacteria isolates has been great concern. The present study was performed to investigate the prevalence co-transferability oqxAB blaCTX-M genes among 696 Escherichia coli (E. coli) from food-producing animals South China, characterize these plasmids. MethodsThe ESBL-encoding (blaCTX-M, blaTEM blaSHV), (qnrA, qnrB, qnrS, qnrC, qnrD, aac(6')-Ib-cr, qepA, oqxAB) were determined by PCR sequenced directionally. Conjugation, S1 nuclease...
Antibiotic resistance is a prevalent problem in public health worldwide. In general, the carbapenem β-lactam antibiotics are considered final resort against lethal infections by multidrug-resistant bacteria. Colistin cationic polypeptide antibiotic and acts as last line of defense for treatment carbapenem-resistant Very recently, new plasmid-borne colistin gene, mcr-2, was revealed soon after discovery paradigm gene mcr-1, which has disseminated globally. However, molecular mechanisms MCR-2...
ABSTRACT We report the complete nucleotide sequence of a plasmid, pA31-12, carrying bla CTX-M-55 and mcr-1 from chicken Escherichia coli isolate. pA31-12 has an IncI2 replicon that displays extensive similarity with pHN1122-1-borne pHNSHP45-borne . Insertion sequences IS Ecp1 Apl1 are responsible for mobilization , respectively. The colocalization extended-spectrum β-lactamase gene on conjugative plasmid may accelerate dissemination both genes by coselection.
Plasmid-mediated antimicrobial resistance has emerged as one of the principal global issues, posing significant threats to public health. Herein, we reported a mobile tigecycline mechanism Tet(X4) on both plasmid and chromosome in Escherichia coli strains from migratory birds China. Besides tigecycline, these tet(X4)-positive also exhibited elevated MICs FDA newly approved tetracycline antibiotics, eravacycline (4 µg/ml) omadacycline (8 µg/ml). Worrisomely, tet(X4)-carrying plasmids shared...
To investigate the prevalence and transmission of mcr-3 among Salmonella enterica serotype Typhimurium 1,4,[5],12:i:-.A total 4724 clinical isolates were screened for presence in China during 2014-19. The clonal relationship mcr-3-positive their plasmid contents complete sequence also characterized based on WGS data from Illumina MinION platforms.We identified 10 isolates, all MDR, mostly resistant to colistin, cefotaxime, ciprofloxacin, doxycycline florfenicol. was co-present with...
This study aimed to determine the global distribution and molecular characteristics of carbapenemase-producing Pseudomonas aeruginosa isolates. A total 328 (11.1%, 328/2953) P. isolates from humans were obtained public databases as October 2019. Of which, blaVIM blaIMP genes most prevalent carbapenemases in These possessed 34 distinct sequence types (STs) six predominated: ST357, ST823, ST308, ST233, ST175 ST111. The ST357 ST823 primarily found detected Asia all Europe. ST233 ST111 spread...
We investigated cfr-positive and -negative MRSA strains isolated from animals humans in different geographical areas of China, 2011 to 2016. Twenty (15.6%) were identified 128 including 17 food three humans. The resistance rates prevalence the tested antibiotic genes (ARGs) isolates higher than that cfr-negative isolates. All co-carrying fexA ermC, had significantly optrA incidence rate vs. (P < 0.05). In addition, multilocus sequence typing (MLST) assays showed ST9 spa-type t899 most...
Antimicrobial resistance is recognized as one of the major global health challenges 21st century. Synergistic combinations for antimicrobial therapies can be a good strategy treatment multidrug resistant infections. We examined ability group 29 plant essential oils substances which enhance antibiotic activity. used modified well diffusion method to establish high-throughput screening easy and rapid identification high-level enhancement against bacteria. found that 25 possessed antibacterial...
Abstract Objectives The emergence and spread of plasmid-encoded tet(X3/X4) genes that confer high-level tigecycline eravacycline resistance in Escherichia coli Acinetobacter spp. pose serious threats to human animal health. We developed a rapid robust assay detect Tet(X3/X4) Gram-negative bacteria based on degradation by the presence Tet(X) enzyme test strain. Methods This tetracycline inactivation method (TIM) is Tet(X3/X4)-producing strain, which results reduced activity against an...
Abstract Objectives To reconstruct the genomic epidemiology and evolution of MDR Salmonella Indiana in China. Methods A total 108 strains were collected from humans livestock All isolates subjected to WGS antimicrobial susceptibility testing. Phylogenetic relationships evolutionary analyses conducted using data this study NCBI database. Results Almost all displayed phenotype. Importantly, 84 possessed concurrent resistance ciprofloxacin cefotaxime. analysis revealed that class 1 integrons on...
We screened 30 Klebsiella pneumoniae isolates from dogs and cats at a single animal hospital in Guangdong Province, China. Among them, 12 K. strains possessed high-level resistance to amikacin gentamicin these were for 16S rRNA methyltransferase (16S-RMTase) genes. And then the genes positive detected ESBLs (extended spectrum β-lactamases) analyzed by pulsed-field gel electrophoresis, multilocus sequence typing, PCR-based replicon typing plasmid analysis. The genetic profiles of rmtB also...
Urinary tract infections (UTI) are common that can be mild to life threatening. However, increased bacterial resistance and poor patient compliance rates have limited the effectiveness of conventional antibiotic therapies. Here, we investigated relationship between nitrofurantoin amikacin against 12 clinical MDR uropathogenic Escherichia coli (UPEC) strains both in vitro an experimental Galleria mellonella model. In synergistic effects were observed all test by standard checkerboard...
The extensive use of tetracycline antibiotics has led to the widespread presence tetracycline-resistance genes in Gram-negative bacteria and this poses serious threats human animal health. In our previous study, we reported a method for rapid detection Tet(X)-producers using MALDI-TOF MS. However, there have been multiple machineries involved resistance including efflux pump, ribosomal protection protein. Our demonstrated limitation probing non-Tet(X)-producing tetracycline-resistant...
Biofilm formations play an important role in Staphylococcus aureus pathogenesis and contribute to antibiotic treatment failures biofilm-associated infections. The aim of this study was evaluate the pharmacokinetic/pharmacodynamic (PK/PD) profiles cefquinome against experimental catheter-related biofilm model due S. aureus, including three clinical isolates one non-clinical isolate. minimal inhibitory concentration (MIC), (MBIC), bactericidal (BBC), eradication (MBEC) prevention (BPC) vitro...
This study reported the involvement of a gene cluster from conjugative plasmid in biofilm formation Escherichia coli. We used novel EZ-Tn5 transposon technique to generate library and arbitrarily primed PCR detect insertion sites formation-deficient mutants. To validate function candidate genes, genes were cloned into pBluescript II SK (+) transformed E. coil DH5α. Biofilm production transformants was then assessed by phenotypic using Crystal Violet staining microscopy. A total 3,000 mutants...
Abstract The spread of antibiotic resistance genes (ARGs), especially those on plasmids, poses a major risk to global health. However, the extent and frequency ARG transfer in microbial communities among human, animal, environmental sectors is not well understood due lack effective tracking tools. We have developed new fluorescent tracing tool, CRISPR-AMRtracker, study transfer. It combines CRISPR/Cas9 fluorescence tagging, fluorescence-activated cell sorting, 16S rRNA gene sequencing,...