A5043804674- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Cancer-related gene regulation
- Neurotransmitter Receptor Influence on Behavior
- Genomics and Phylogenetic Studies
- Genetics and Neurodevelopmental Disorders
- Genomic variations and chromosomal abnormalities
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Adipose Tissue and Metabolism
- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Alcohol Consumption and Health Effects
- Birth, Development, and Health
- Receptor Mechanisms and Signaling
- Folate and B Vitamins Research
- Trace Elements in Health
- Vitamin C and Antioxidants Research
- Peroxisome Proliferator-Activated Receptors
- Neuroscience and Neuropharmacology Research
- Heavy Metal Exposure and Toxicity
- Bacillus and Francisella bacterial research
Sorbonne Université
2024
Institut Curie
2019-2024
Centre National de la Recherche Scientifique
2019-2024
Université Paris Sciences et Lettres
2019-2024
Dynamique du noyau
2024
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2023
University of Chile
2014-2022
Abstract DNA methylation is an essential epigenetic chromatin modification, and its maintenance in mammals requires the protein UHRF1. It yet unclear if UHRF1 functions solely by stimulating DNMT1, or it has important additional functions. Using degron alleles, we show that depletion causes a much greater loss of than DNMT1 depletion. This not caused passive demethylation as UHRF1-depleted cells proliferate more slowly DNMT1-depleted cells. Instead, bioinformatics, proteomics genetics...
DNA methylation (DNAme) is a key epigenetic mark that regulates critical biological processes maintaining overall genome stability. Given its pleiotropic function, studies of DNAme dynamics are crucial, but currently available tools to interfere with have limitations and major cytotoxic side effects. Here, we present cell models allow inducible reversible modulation through DNMT1 depletion. By dynamically assessing whole locus-specific effects induced passive demethylation divisions, reveal...
Background A number of studies have shown that acetaldehyde synthesized in the brain is necessary to induce ethanol (EtOH) reinforcement naïve animals (acquisition phase). However, after chronic intake achieved (maintenance phase), EtOH becomes independent generation or its levels. Glutamate has been reported be associated with maintenance intake. The levels extracellular glutamate are modulated by 2 glial processes: reabsorption via an Na+-glutamate transporter (GLT1) and a...
We have previously shown that the administration of fenofibrate to high-drinker UChB rats markedly reduces voluntary ethanol intake. Fenofibrate is a PPAR agonist, which induces proliferation peroxisomes in liver, leading increases catalase levels result acetaldehyde accumulation at aversive blood when animals consume ethanol. In these new studies, we aimed investigate if effect on intake produced exclusively liver (increasing and systemic acetaldehyde) or there might be additional effects...
Loxoscelism is the envenomation caused by bite of Loxosceles spp. spiders. It entails severe necrotizing skin lesions, sometimes accompanied systemic reactions and even death. There are no diagnostic means treatment mostly palliative. The main toxin, found in several isoforms venom, sphingomyelinase D (SMD), a phospholipase that has been used to generate antibodies intended for medical applications. Nucleic acid aptamers promising alternative antibodies. Aptamers may be isolated from...
ABSTRACT DNA methylation (DNAme) is a key epigenetic mark that regulates critical biological processes maintaining overall genome stability. Given its pleiotropic function, studies of DNAme dynamics are crucial, but currently available tools to interfere with have limitations and major cytotoxic side effects. Here, we present untransformed cancer cell models allow inducible reversible global modulation through DNMT1 depletion. By dynamically assessing the effects induced passive...
Centromeres are key elements for chromosome segregation. Canonical centromeres built over long-stretches of tandem repetitive arrays. Despite being quite abundant compared to other loci, centromere sequences overall still represent only 2 5% the human genome, therefore studying their genetic and epigenetic features is a major challenge. Furthermore, sequencing centromeric regions requires high coverage fully analyze length sequence variations, this can be extremely costly. To bypass these...
Background: Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) ionic channels are known to play a key role in the control of neuron excitability and have been proposed as molecular target ethanol. Previous studies rats shown that gene-induced overexpression HCN2 channel ventral tegmental area (VTA) increases rewarding effects ethanol its intake by animals.Objective: The aim this work was study VTA gene knockdown voluntary consumption alcohol-preferring UChB rats.Methods: Two...
Abstract DNA methylation is an essential epigenetic chromatin modification, and its maintenance in mammals requires the protein UHRF1. It yet unclear if UHRF1 functions solely by stimulating DNMT1, or it has important additional functions. Using degron alleles, we show that depletion causes a much greater loss of than DNMT1 depletion. This not caused passive demethylation as UHRF1-depleted cells proliferate more slowly DNMT1-depleted cells. Instead, bioinformatics, proteomics genetics...
Abstract DNA methylation is an essential epigenetic chromatin modification, and its maintenance in mammals requires the protein UHRF1. It yet unclear if UHRF1 functions solely by stimulating DNMT1, or it has important additional functions. Using degron alleles, we show that depletion causes a much greater loss of than DNMT1 depletion. This not caused passive demethylation as UHRF1-depleted cells proliferate more slowly DNMT1-depleted cells. Instead, bioinformatics, proteomics genetics...