A5075153606- Cancer Immunotherapy and Biomarkers
- Angiogenesis and VEGF in Cancer
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Immune cells in cancer
- COVID-19 Clinical Research Studies
- MicroRNA in disease regulation
- Mechanical Circulatory Support Devices
- Pulmonary Hypertension Research and Treatments
- Nanoparticle-Based Drug Delivery
- Oceanographic and Atmospheric Processes
- Ocean Waves and Remote Sensing
- Diet and metabolism studies
- Cervical Cancer and HPV Research
- Head and Neck Cancer Studies
- Meteorological Phenomena and Simulations
- Respiratory Support and Mechanisms
- Immunotherapy and Immune Responses
- Venous Thromboembolism Diagnosis and Management
- Cell Image Analysis Techniques
- Cardiac Structural Anomalies and Repair
- Nanoplatforms for cancer theranostics
Sorbonne Paris Cité
2011-2024
Université Paris Cité
2014-2024
Inserm
2015-2024
Institut Curie
2007-2024
Université Paris Sciences et Lettres
2019-2024
Centre Hospitalier Universitaire de Grenoble
2024
Délégation Paris 5
2011-2023
Luxembourg Institute of Health
2016-2023
CEA Grenoble
2012-2023
Université Grenoble Alpes
2012-2023
Head and neck cancers positive for human papillomavirus (HPV) have a more favorable clinical outcome than HPV-negative cancers, but it is unknown why this the case. We hypothesized that prognosis was affected by intrinsic features of HPV-infected tumor cells or differences in host immune response. In study, we focused on comparison regulatory Foxp3(+) T programmed death-1 (PD-1)(+) microenvironment tumors were negative HPV, two groups matched various biologic parameters. HPV-positive head...
Rationale for Study: MicroRNAs (miRNAs) are small noncoding RNAs that regulate protein expression at post-transcriptional level. We hypothesized a specific pool of endothelial miRNAs could be selectively regulated by flow conditions and inflammatory signals, as such involved in the development atherosclerosis. Objective: To identify miRNAs, called atheromiRs, which shear stress oxidized low-density lipoproteins (oxLDL), to determine their role atherogenesis. Methods Results: Large-scale...
Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and pathophysiological conditions are increasingly recognised as potential biomarkers, therapeutic agents drug delivery vehicles. Based on their size biogenesis, EVs classified exosomes, microvesicles apoptotic bodies. Due to overlapping ranges the lack of specific markers, these classes cannot yet be distinguished experimentally. Currently, it is a major challenge field define robust sensitive technological...
Coronavirus disease-2019 (COVID-19), a respiratory disease has been associated with ischemic complications, coagulation disorders, and an endotheliitis. To explore endothelial damage activation-related biomarkers in COVID-19 patients criteria of hospitalization for referral to intensive care unit (ICU) and/or worsening. Analysis angiogenic soluble markers plasma from at admission. Study enrolled 40 consecutive admitted emergency department that fulfilled hospitalization. Half them were...
To examine the relation of endothelial microparticles (EMPs) with cardiometabolic risk in community. Circulating EMPs are small membrane vesicles released after cell injury. Endothelial reportedly increased among individuals a high burden cardiovascular factors. However, prior investigations have been limited to small, highly selected samples. We studied 844 without history disease Framingham Offspring cohort (mean age 66 ± 9 years, 57% women). used standardized flow cytometry methods...
A rapid and massive influx of inflammatory cells occurs into ischemic area after myocardial infarction (MI), resulting in local release cytokines growth factors. Yet, the mechanisms regulating their production are not fully explored. The extracellular vesicles (EVs) interstitial space curbs important biological functions, including inflammation, influences development cardiovascular diseases. To date, there is no evidence for situ cardiac EVs MI.The present study tested hypothesis that EV...
Significance The failure in achieving a durable clinical immune response against cancer cells depends on the ability of to establish microenvironment that prevent cytotoxic infiltrate tumors and kill cells. Therefore, key approach successful antitumor is harness strategies allowing reorientation tumor. Herein we reveal inhibiting autophagy induces massive infiltration natural killer into tumor bed, subsequent dramatic decrease volume melanomas. These results highlight role targeting breaking...
Significance Our work uncovers mechanisms by which tumor cells impact tumor-associated macrophages in human triple-negative breast cancer. Via extracellular vesicles (EVs), these tumors promote with proinflammatory features, correlated better clinical outcome. results suggest exploration of EVs as tools, alone, or combination other therapies, to a favorable environment for the generation anti-tumor immune responses.
Abstract Exosomes are secreted vesicles formed in late endocytic compartments. Mature dendritic cells (DCs) secrete exosomes bearing functional MHC-peptide complexes and high levels of ICAM-1. Such can activate Ag-specific naive T but only after recapture by recipient APCs. In this study, we addressed the molecular mechanisms interaction between DCs. We show that be presented mouse DCs without need for internalization processing. interact with through a specific saturable receptor. Although...
<h3>Objective</h3> Previous studies suggested that microRNA-21 may be upregulated in the liver non-alcoholic steatohepatitis (NASH), but its role development of this disease remains unknown. This study aimed to determine NASH. <h3>Design</h3> We inhibited or suppressed different mouse models NASH: (a) low-density lipoprotein receptor-deficient (<i>Ldlr</i><sup>−/−</sup>) mice fed a high-fat diet and treated with antagomir-21 antagomir control; (b) microRNA-21-deficient wild-type...
Elucidation of the underlying molecular mechanisms immune evasion in cancer is critical for development immunotherapies aimed to restore and stimulate effective antitumor immunity. Here, we evaluate role actin cytoskeleton breast cell resistance cytotoxic natural killer (NK) cells. A significant fraction cells responded NK-cell attack via a surprisingly rapid massive accumulation F-actin near immunologic synapse, process termed "actin response." Live-cell imaging provided direct evidence...
Acute myocardial infarction (MI) is a severe ischemic disease responsible for heart failure and sudden death. Inflammatory cells orchestrate postischemic cardiac remodeling after MI. Studies using mice with defective mast/stem cell growth factor receptor c-Kit have suggested key roles mast (MCs) in remodeling. Because mutations affect multiple types of both immune nonimmune origin, we addressed the impact MCs on function MI, c-Kit-independent MC-deficient (Cpa3(Cre/+)) mice. In response to...
Summary Very small embryonic-like stem cells (VSELs) are multipotent localised in adult bone marrow (BM) that may be mobilised into peripheral blood (PB) response to tissue injury. We aimed quantify VSELs BM and PB of patients with critical limb ischaemia (CLI) test their angiogenic potential vitro as well therapeutic capacity mouse model CLI. isolated from CLI studied differentiate vascular lineages. Flow imaging cytometry showed VSEL counts were lower (p< 0.001) higher compared healthy...
Abstract Background Microvascular, arterial and venous thrombotic events have been largely described during severe coronavirus disease 19 (COVID-19). However, mechanisms underlying hemostasis dysregulation remain unclear. Methods We explored two independent cross-sectional cohorts to identify soluble markers gene-expression signatures that discriminated COVID-19 severity outcomes. Results found elevated (s)P-selectin at admission was associated with severity. Elevated sP-selectin predictive...
DNA methylation (DNAme) is a key epigenetic mark that regulates critical biological processes maintaining overall genome stability. Given its pleiotropic function, studies of DNAme dynamics are crucial, but currently available tools to interfere with have limitations and major cytotoxic side effects. Here, we present cell models allow inducible reversible modulation through DNMT1 depletion. By dynamically assessing whole locus-specific effects induced passive demethylation divisions, reveal...
The triggering receptor expressed on myeloid cells 1 (TREM-1) drives inflammatory responses in several cardiovascular diseases but its role abdominal aortic aneurysm (AAA) remains unknown. Our objective was to explore the of TREM-1 a mouse model angiotensin II-induced (AngII-induced) AAA. expression detected and colocalized with macrophages. Trem1 gene deletion (Apoe-/-Trem1-/-), as well pharmacological blockade LR-12 peptide, limited both AAA development severity. attenuated response aorta,...